Inner structure of intact chloroplasts observed by a low temperature laser scanning microscope

Title:

Inner structure of intact chloroplasts observed by a low temperature laser scanning microscope

Creator:

Vácha, F., Vácha, M., Bumba, L., Hashizume, K., and Tani, T.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:a594b89e-254b-4aa9-9ce1-9b0d3d0a7432
uuid:a594b89e-254b-4aa9-9ce1-9b0d3d0a7432
issn:0300-3604
doi:10.1023/A:1012492919852

Subject:

chlorophyll fluorescence, grana, photosystems 1 and 2, Spinacia, and thylakoid

Type:

model:article and TEXT

Format:

bez média and svazek

Description:

Inner structure of isolated intact chloroplasts was observed for the first time by a method of laser scanning microscopy at the temperature of liquid nitrogen at 77 K. The microscope, based on gradient index optics, has a maximum resolution of 440 nm at the wavelength of 650 nm. Chloroplasts were excited into the Q-band of chlorophyll b by a krypton laser line at 647.6 nm and fluorescence was detected using two different interference filters. The 680 nm interference filter detects the regions where photosystem (PS) 2 mainly occurs, the 730 nm interference filter detects domains with predominant location of PS1. Since PS1 occurs mainly in stroma lamellae, whereas PS2 occurs mainly in grana regions we were able to view the structure of thylakoid membrane in isolated intact chloroplast that is the closest to in vivo state. and F. Vácha ... [et al.].

Language:

Multiple languages

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Coverage:

493-496

Source:

Photosynthetica | 2000 Volume:38 | Number:4

Harvested from:

CDK

Metadata only:

false