Influence of exogenously applied 6-benzylaininopunne on the structure of chloroplasts and arrangement of their membranes

Title:

Influence of exogenously applied 6-benzylaininopunne on the structure of chloroplasts and arrangement of their membranes

Creator:

Wilhelmová, N. and Kutík, J.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:a80c27b9-4b65-4669-9ffc-7fd214e62151
uuid:a80c27b9-4b65-4669-9ffc-7fd214e62151
urnnbn:urn:nbn:cz:aba007-000fpe

Subject:

carotenoids, thylakoid stacking, starch inclusions, proteins, Nicotiana tabacum\ plastoglobuli, electron microscopy, digitonin fractionation, cytokinin, and chlorophyll

Type:

model:internalpart and TEXT

Format:

print

Description:

The influence of a cytokinin, 6-benzylaminopurine (BAP), on chloroplast structure was studied using biochemical methods and electron microscopy. The average degree of thylakoid stacking was determined by digitonin fractionation and differential centrifugation of chloroplasts from tobacco plantlets after treatment with different concentrations of BAP in agar medium during cultivation in viíro. An elevated concentration of BAP in the medium induced an increase in grana stacking. This was in accordance with the lowering of the chlorophyll a/b ratio in these chloroplasts. The relative amoímt of proteins and carotenoids increased in both stromal and (to a lesser extent) granal chloroplast thylakoid fractions with the BAP concentration. The electron microscopic studies revealed nearly the same volume density of thylakoid membranes within chloroplasts of BAP treated plantlets and control ones. In the BAP treated plantlets the chloroplasts were smaller and had a profound accumulation of starch inclusions and a more flattened shape than the chloroplasts of control plantlets. The volume density of plastoglobuli in chloroplasts did not decrease under the influence of BAP,

Language:

Multiple languages

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Coverage:

559-570 and 81-92

Source:

Photosynthetica | 1995 Volume:31 | Number:4

Harvested from:

CDK

Metadata only:

false