Isolation and characterization of an oxygen evolving photosystem 2 core complex from the thermophilic cyanobacterium Mastigocladus laminosus

Title:

Isolation and characterization of an oxygen evolving photosystem 2 core complex from the thermophilic cyanobacterium Mastigocladus laminosus

Creator:

Ramesh, V. M., Fish, A., Michaeli, D., Keren, N., Ohad, I., Vorchovsky, L., and Nechushtai, R.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:b0d36e01-3fb6-4613-afec-f0487b05b194
uuid:b0d36e01-3fb6-4613-afec-f0487b05b194
issn:0300-3604
doi:10.1023/A:1022666706700

Subject:

2,6-dimethylbenzoquinone, fluorescence, membrane proteins, oxygen evolution rate, reaction center, and thermoluminescence

Type:

model:article and TEXT

Format:

bez média and svazek

Description:

A novel purification procedure was developed for the isolation of oxygen evolving photosystem 2 (PS2) from Mastigocladus laminosus. The isolation procedure involves dodecyl maltoside extraction followed by column chromatography using anion exchange resins. The isolated PS2 reaction center (RC) was analyzed for its biochemical and biophysical characteristics. Analysis by SDS polyacrylamide gel electrophoresis revealed that the complex contained five intrinsic membrane proteins (CP 47, CP 43, D1, D2, and cyt b559) and at least three low molecular mass proteins. The complex exhibited high rates of oxygen evolution [333 mmol(O2) kg-1(Chl) s-1] in the presence of 2.5 mM 2,6-dimethylbenzoquinone (DMBQ) as an artificial electron acceptor. The red chlorophyll a absorption peak of this complex was observed at 673.5±0.2 nm. The isolated PS2 core complex was free of photosystem 1 as inferred from its SDS-PAGE and fluorescence spectrum. The electron transfer properties of the Mastigocladus cells and the purified PS2 core complex were further probed by measuring thermoluminescence signals, which indicated the presence of a primary quinone electron acceptor (QA) in the purified PS2 core complex. and V. M. Ramesh ... [et al.].

Language:

Multiple languages

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Coverage:

355-361

Source:

Photosynthetica | 2002 Volume:40 | Number:3

Harvested from:

CDK

Metadata only:

false