High-light-induced superoxide anion radical formation in cytochrome b6f complex from spinach as detected by EPR spectroscopy

Title:

High-light-induced superoxide anion radical formation in cytochrome b6f complex from spinach as detected by EPR spectroscopy

Creator:

Sang, M., Qin, X.-C., Wang, W.-D., Xie, J., Chen, X.-B., Wang, K. B., Zhang, J.-P., Li, L.-B., and Kuang, T. Y.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:b3eb8010-18b8-4118-bd74-f77ba261a7a5
uuid:b3eb8010-18b8-4118-bd74-f77ba261a7a5
issn:0300-3604
doi:10.1007/s11099-011-0008-0

Subject:

botanika, botany, Cyt b6f, EPR, photoprotection, and superoxide anion radical

Type:

model:article and TEXT

Format:

bez média and svazek

Description:

The generation of superoxide anion radical (O2.-) in the cytochrome b6f complex (Cyt b6f) of spinach under high-light illumination was studied using electron paramagnetic resonance spectroscopy. The generation of O2.- was lost in the absence of molecular oxygen. It was also suppressed in the presence of NaN3 and could be scavenged by extraneous antioxidants such as ascorbate, β-carotene, and glutathione. The results also indicate that O2.-, which is produced under high-light illumination of the Cyt b6f from spinach, might be generated from a reaction involing 1O2, and the Rieske Fe-S protein could serve as the electron donor in the O2.- production. The mechanism of photoprotection of the Cyt b6f complex by antioxidants is discussed. and M. Sang ... [et al.].

Language:

Multiple languages

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Coverage:

48-54

Source:

Photosynthetica | 2011 Volume:49 | Number:1

Harvested from:

CDK

Metadata only:

false