Application of spectrally resolved fluorescence induction to study light-induced nonphotochemical quenching in algae

Title:

Application of spectrally resolved fluorescence induction to study light-induced nonphotochemical quenching in algae

Creator:

Kaňa, Radek

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:bb188f85-6b20-40bd-b7d6-1a2e5efc1475
uuid:bb188f85-6b20-40bd-b7d6-1a2e5efc1475
issn:0300-3604
doi:10.1007/s11099-018-0780-1

Subject:

ochrana před ultrafialovým zářením, fotosyntéza, photoprotection, photosynthesis, fluorescence parameters, light-harvesting complex, photosystem II, 2, and 581

Type:

model:article and TEXT

Format:

print, bez média, and svazek

Description:

The light-induced nonphotochemical quenching (NPQ) can safely dissipate excess of absorbed light to heat. Here we describe an application of spectrally resolved fluorescence induction (SRFI) method for studying spectral variability of NPQ. The approach allows detection of spectrally-resolved nonphotochemical quenching (NPQλ) representing NPQ dependency on fluorescence emission wavelength in the whole spectral range of fluorescence emission. The experimental approach is briefly described and NPQλ is studied for the cryptophyte alga Rhodomonas salina and for green alga Chlorella sp. We confirm presence of NPQλ only in membrane-bound antennae (chlorophyll a/c antennae) and not in phycobiliproteins in lumen in cryptophyte and show that NPQλ is inhibited in the whole spectral range by NPQ inhibitors in Chlorella sp. We discuss variability in the quenching in the particular spectral ranges and applicability of the NPQλ parameter to study quenching locus in vivo., R. Kaňa., and Obsahuje bibliografické odkazy

Language:

Multiple languages

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Coverage:

132-138

Source:

Photosynthetica | 2018 Volume:56 | Number:1

Harvested from:

CDK

Metadata only:

false