Differences in cold inactivation of phosphoenolpyruvate carboxylase among C4 species: The effect of pH and of enzyme concentration

Title:

Differences in cold inactivation of phosphoenolpyruvate carboxylase among C4 species: The effect of pH and of enzyme concentration

Creator:

Zervoudakis, G., Angelopoulos, K., Salahas, G., and Georgiou, C. D.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:be22b52b-dd0d-44ba-9e86-ba4d4dd99b0c
uuid:be22b52b-dd0d-44ba-9e86-ba4d4dd99b0c
issn:0300-3604
doi:10.1023/A:1006998420652

Subject:

Amaranthus sp., Atriplex halimus, Cynodon dactylon, Digitaria sanguinalis, Portulaca oleracea, Saccharum officinarum, Salsola kali, Setaria verticillata, and Zea mays

Type:

model:article and TEXT

Format:

bez média and svazek

Description:

Among various C4 plants we found a wide range in the level of inactivation of phosphoenolpyruvate carboxylase (PEPC) at low temperature (0 °C). The activity of the 2-fold diluted enzyme in crude leaf extracts after 60 min incubation (compared to zero time incubation) at pH 7.5, remained above 87 % at low temperatures for the species Setaria verticillata, Portulaca oleracea, and Saccharum officinarum, and between 11 and 17 % in the species Cynodon dactylon and Atriplex halimus. The enzyme exhibited intermediate levels of inactivation (42 to 58 %) for the species Amaranthus sp., Zea mays, Salsola kali, and Digitaria sanguinalis. The enzyme activity for S. verticillata was unaffected between pH 5.7 and 8.4 during incubation at room and low temperatures. Under similar conditions, the activity of the enzyme from C. dactylon was stable between pH 5.7 and 7.0 and decreased at pH above 7.0, but for Z. mays it was enhanced between pH 5.7 and 6.8 and decreased at pH above 7.0. and G. Zervoudakis ... [et al.].

Language:

Multiple languages

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Coverage:

169-175

Source:

Photosynthetica | 1998 Volume:35 | Number:2

Harvested from:

CDK

Metadata only:

false