Changes in chloroplast ultrastructure in Pssu-ipt tobacco during plant ontogeny

Title:

Changes in chloroplast ultrastructure in Pssu-ipt tobacco during plant ontogeny

Creator:

Synková, H., Pechová, R., and Valcke, R.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:c748f4a7-f86a-4839-ad57-d8fe704368a8
uuid:c748f4a7-f86a-4839-ad57-d8fe704368a8
issn:0300-3604
doi:10.1023/A:1025829017298

Subject:

chloroplast ultrastructure, cytokinins, grafting, granum, leaf age, Nicotiana, peroxisome, phytohormones, thylakoid, and transgenic plants

Type:

model:article and TEXT

Format:

bez média and svazek

Description:

Changes in chloroplast ultrastructure and total content of endogenous cytokinins (CK) were studied during different phases of plant development in transgenic Pssu-ipt tobacco (Nicotiana tabacum L. cv. Petit Havana SR1). Permanent overproduction of CK was found in both rooted (SE) and grafted (G) Pssu-ipt plants in all phases of plant development with the peak in vegetative and flowering phase in the latter ones. No such a correlation was observed in SE on the contrary to control non-transgenic plants (SR1) and grafts (SRG), which showed also CK increase at juvenile and flowering phases. No significant differences in parameters of chloroplast ultrastructure, such as length of chloroplast, starch content, granum width, and number of thylakoids per granum, were proved between chloroplasts from young mature leaves of control and transgenic tobacco during plant ontogeny. Nevertheless, several anomalies in the ultrastructure of cell organelles were found in Pssu-ipt tobacco. Amoeboid shape of chloroplasts was often observed in connection with "tubular clusters" resembling peripheral reticulum. The distinct crystalline structures located in chloroplasts might be formed by LHC protein aggregates. Smaller crystals of unknown composition were found also in mitochondria. Numerous crystalline cores were present in peroxisomes. The alterations might be the result of imbalance of phytohormone content, degradation effect of CK overproduction, or the example of acclimation to permanent stress. and H. Synková, R. Pechová, R. Valcke.

Language:

Multiple languages

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Coverage:

117-126

Source:

Photosynthetica | 2003 Volume:41 | Number:1

Harvested from:

CDK

Metadata only:

false