Secondary structure estimation of recombinant psbH, encoding a photosynthetic membrane protein of cyanobacterium Synechocystis sp. PCC 6803

Title:

Secondary structure estimation of recombinant psbH, encoding a photosynthetic membrane protein of cyanobacterium Synechocystis sp. PCC 6803

Creator:

Štys, D., Schoefberger, W., Halbhuber, Z., Ristvejova, J., Müller, N., and Ettrich, R.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:c7b6e73c-d80f-4461-8fa5-81cc0d676f10
uuid:c7b6e73c-d80f-4461-8fa5-81cc0d676f10
issn:0300-3604
doi:10.1007/s11099-005-0067-1

Subject:

CD spectroscopy, molecular dynamics calculations, NMR spectroscopy, photosystem 2, and protein folding

Type:

model:article and TEXT

Format:

bez média and svazek

Description:

The PsbH protein of cyanobacterium Synechocystis sp. PCC 6803 was expressed as a fusion protein with glutathione-S transferase (GST) in E. coli grown on a mineral medium enriched in 15N isotope. After enzymatic cleavage of the fusion protein, the 1H-15N-HSQC spectrum of PsbH protein in presence of the detergent β-D-octyl-glucopyranoside (OG) was recorded on a Bruker DRX 500 MHz NMR spectrometer equipped with a 5 mm TXI cryoprobe to enhance the sensitivity and resolution. Non-labelled protein was used for secondary structure estimation by deconvolution from circular dichroism (CD) spectra. Experimental results were compared with our results from a structural model of PsbH using a restraint-based comparative modelling approach combined with molecular dynamics and energetic modelling. We found that PsbH shows 34-38% α-helical structure (Thr36-Ser60), a maximum of around 15% of β-sheet, and 12-19% of β-turn. and D. Štys ... [et al.].

Language:

Multiple languages

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Coverage:

421-424

Source:

Photosynthetica | 2005 Volume:43 | Number:3

Harvested from:

CDK

Metadata only:

false