Changes in electron transport, superoxide dismutase and ascorbate peroxidase isoenzymes in chloroplasts and mitochondria of cucumber leaves as influenced by chilling

Title:

Changes in electron transport, superoxide dismutase and ascorbate peroxidase isoenzymes in chloroplasts and mitochondria of cucumber leaves as influenced by chilling

Creator:

Hu, W. H., Song, X. S., Shi, K., Xia, X. J., Zhou, Y. H., and Yu, J. Q.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:c8148d67-ce34-4bd5-b309-7b9868d51ff0
uuid:c8148d67-ce34-4bd5-b309-7b9868d51ff0
issn:0300-3604
doi:10.1007/s11099-008-0098-5

Subject:

ascorbate peroxidase, Cucumis sativus, net photosynthetic rate, photosynthetic electron transport, quantum efficiency, reactive oxidative metabolism, respiratory electron transport, respiration pathways, and superoxide dismutase

Type:

model:article and TEXT

Format:

bez média and svazek

Description:

In order to clarify the relationship between chill-induced disturbance in photosynthetic, respiratory electron transport and the metabolism of reactive oxygen species (ROS), leaf gas exchange, chlorophyll fluorescence quenching, respiration, and activities of superoxide dismutase (SOD) and ascorbate peroxidase (APX) were investigated in chloroplasts and mitochondria of cucumber (Cucumis sativus) leaves subjected to a chill (8 °C) for 4 d. Chilling decreased net photosynthetic rate (PN) and quantum efficiency of photosystem 2 (ΦPS2), but increased the ratio of ΦPS2 to the quantum efficiency of CO2 fixation (ΦCO2) and non-photochemical quenching (NPQ) in cucumber leaves. While chilling inhibited the activity of cytochrome respiration pathway, it induced an increase of alternative respiration pathway activity and the reduction level of Q-pool. Chilling also significantly increased O2* production rate, H2O2 content, and SOD and APX activities in chloroplasts and mitochondria. There was a more significant increase in SOD and APX activities in chloroplasts than in mitochondria with the increase of membrane-bound Fe-SOD and tAPX in chloroplasts being more significant than other isoenzymes. Taken together, chilling inhibited PN and cytochrome respiratory pathway but enhanced the photosynthetic electron flux to O2 and over-reduction of respiratory electron transport chain, resulting in ROS accumulation in cucumber leaves. Meanwhile, chilling resulted in an enhancement of the protective mechanisms such as thermal dissipation, alternative respiratory pathway, and ROS-scavenging mechanisms (SODs and APXs) in chloroplasts and mitochondria. and W. H. Hu ... [et al.].

Language:

Multiple languages

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Coverage:

581-588

Source:

Photosynthetica | 2008 Volume:46 | Number:4

Harvested from:

CDK

Metadata only:

false