Temperature dependent UV-Vis spectral changes in hydrogen-and deuterium-bonded photosynthetic reaction centers of Rhodobacter sphaeroides

Title:

Temperature dependent UV-Vis spectral changes in hydrogen-and deuterium-bonded photosynthetic reaction centers of Rhodobacter sphaeroides

Creator:

Ostafin, A. E., Popova, J. A., Payne, C. K., Mizukami, H., and Norris, J. R.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:f0c73838-3887-4f70-b789-b8c6b0ddc579
uuid:f0c73838-3887-4f70-b789-b8c6b0ddc579
issn:0300-3604
doi:10.1007/s11099-006-0047-0

Subject:

enthalpy, entropy, ferricyanide, 1,3,5-hexanetriol, and photosynthetic bacteria

Type:

model:article and TEXT

Format:

bez média and svazek

Description:

The UV-Vis absorption spectra of detergent-isolated hydrogen-and deuterium-bonded reaction centers (RCs) from Rhodobacter sphaeroides PUC 705Ba were examined as a function of temperature between 20 and 55 °C. The enthalpy and entropy of denaturation for the specimens was determined, revealing that their process of thermal denaturation is significantly different. Deuterium-bonded RCs are most stable at 37 °C, rather than at room temperature, and undergo a "cold denaturation" as the temperature is lowered to room temperature. At room temperature the addition of 1,3,5-heptanetriol brought the deuterium-bonded RC back to its more stable configuration. Hence the hydrogen bonding interactions in the RC do influence its conformation and this is reflected in the microenvironment of its associated pigments. and A. E. Ostafin ... [et al.].

Language:

Multiple languages

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Coverage:

433-438

Source:

Photosynthetica | 2006 Volume:44 | Number:3

Harvested from:

CDK

Metadata only:

false