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2. A novel stage-specific glycosomal nucleoside diphosphate kinase from Trypanosoma cruzi
- Creator:
- Cámara, María de los Milagros, Bouvier, León, Reigada, Chantal, Digirolamo, Fabio A, Sayé, Melisa, and Pereira, Claudio A
- Format:
- print, počítač, and online zdroj
- Type:
- model:article and TEXT
- Subject:
- energetický metabolismus, energy metabolism, trypanosomatids, Chagas disease, glycosomes, trypomastigotes, 2, and 59
- Language:
- English
- Description:
- Nucleoside diphosphate kinases (NDPK) are key enzymes involved in the intracellular nucleotide maintenance in all living organisms, especially in trypanosomatids which are unable to synthesise purines de novo. Four putative NDPK isoforms were identified in the Trypanosoma cruzi Chagas, 1909 genome but only two of them were characterised so far. In this work, we studied a novel isoform from T. cruzi called TcNDPK3. This enzyme presents an atypical N-terminal extension similar to the DM10 domains. In T. cruzi, DM10 sequences targeted other NDPK isoform (TcNDPK2) to the cytoskeleton, but TcNDPK3 was localised in glycosomes despite lacking a typical peroxisomal targeting signal. In addition, TcNDPK3 was found only in the bloodstream trypomastigotes where glycolytic enzymes are very abundant. However, TcNDPK3 mRNA was also detected at lower levels in amastigotes suggesting regulation at protein and mRNA level. Finally, 33 TcNDPK3 gene orthologs were identified in the available kinetoplastid genomes. The characterisation of new glycosomal enzymes provides novel targets for drug development to use in therapies of trypanosomatid associated diseases., María de los Milagros Cámara, León Bouvier, Chantal Reigada, Fabio A. Digirolamo, Melisa Sayé, Claudio A. Pereira., and Obsahuje bibliografii
- Rights:
- http://creativecommons.org/publicdomain/mark/1.0/ and policy:public
3. An eight-year survey of the intestinal parasites of carnivores, hoofed mammals, primates, ratites and reptiles in the Ljubljana zoo in Slovenia
- Creator:
- Kvapil, Pavel, Kastelic, Marjan, Dovč, Alenka, Eva Bártová, Petr Čížek, Lima, Natacha, and Štrus, Špela
- Format:
- print, počítač, and online zdroj
- Type:
- model:article and TEXT
- Subject:
- zvířata chovaná v ZOO, zoo animals, helminths, protists, long-term monitoring, coprology, 2, and 59
- Language:
- English
- Description:
- Problems with parasitic infections and their interspecies transmissions are common in zoological gardens and could pose serious health damage to captive animals. This study presents results of eight-year monitoring of intestinal parasites in animals from Zoo Ljubljana, Slovenia. A total of 741 faecal samples from 40 animal species were collected two to four times per year and examined microscopically. Intestinal parasites were detected in 45% of samples, with detection of helminths (Cestoda, Nematoda - Ascaridida, Enoplida, Strongylida, Oxyurida, Rhabditida and Trichurida) and protists (Apicomplexa and Ciliophora) in 25% and 13% of samples, respectively; mixed infection was found in 7% of samples. The mostly infected were ungulates (61%), followed by reptiles (44%), ratites (29%), primates (22%) and carnivores (7%). During the observation period, the number of infected animal species increased from 8 to 25. This is the first long-term monitoring study of intestinal parasites in zoo animals from Slovenia. Routine monitoring of parasitic infection and regular deworming and hygienic measures are necessary to prevent gastrointestinal infections in captive animals., Pavel Kvapil, Marjan Kastelic, Alenka Dovč, Eva Bártová, Petr Čížek, Natacha Lima, Špela Štrus., and Obsahuje bibliografii
- Rights:
- http://creativecommons.org/publicdomain/mark/1.0/ and policy:public
4. Application of compound mixture of caprylic acid, iron and mannan oligosaccharide against Sparicotyle chrysophrii (Monogenea: Polyopisthocotylea) in gilthead sea bream, Sparus aurata
- Creator:
- Rigos, George, Mladineo, Ivona, Nikoloudaki, Chrysa, Vrbatovic, Anamarija, and Kogiannou, Dimitra
- Format:
- print, počítač, and online zdroj
- Type:
- model:article and TEXT
- Subject:
- parazitologie, parasitology, monogeneans, treatment, Sparidae, MOS, 2, and 59
- Language:
- English
- Description:
- We have evaluated the therapeutic effect of a compound mixture of caprylic acid (200 mg/kg fish), organic iron (0.2% of diet) and mannan oligosaccharide (0.4% of diet) in gilthead sea bream, Sparus aurata Linnaeus, infected with Sparicotyle chrysophrii Beneden et Hesse, 1863 in controlled conditions. One hundred and ten reared and S. chrysophrii-free fish (197 g) located in a cement tank were infected by the parasite two weeks following the addition of 150 S. chrysophrii-infected fish (70 g). Growth parameters and gill parasitic load were measured in treated against control fish after a ten-week-period. Differences in final weight, feed conversion ratio, specific growth rate and feed efficiency were not statistically significant between the experimental groups, suggesting no evident effect with respect to fish growth during the study period. Although the prevalence of S. chrysophrii was not affected by the mixture at the end of the experiment, the number of adults and larvae was significantly lower. The mean intensity encompassing the number of adults and larvae was 8.1 in treated vs 17.7 in control fish. Individual comparisons of gill arches showed that the preferred parasitism site for S. chrysophrii it the outermost or fourth gill arch, consistently apparent in fish fed the modified diet and in control fish. In conclusion, the combined application of caprylic acid, organic iron and mannan oligosaccharide can significantly affect the evolution of infection with S. chrysophrii in gilthead sea bream, being capable of reducing adult and larval stages of the monogenean. However, no difference in growth improvement was observed after the trial period, potentially leaving space for further optimisation of the added dietary compounds., George Rigos, Ivona Mladineo, Chrysa Nikoloudaki, Anamarija Vrbatovic, Dimitra Kogiannou., and Obsahuje bibliografii
- Rights:
- http://creativecommons.org/publicdomain/mark/1.0/ and policy:public
5. Canine thelaziosis in the Czech Republic: the northernmost autochthonous occurrence of the eye nematode Thelazia callipaeda Railliet et Henry, 1910 in Europe
- Creator:
- Milan Jirků, Roman Kuchta, Gricaj, Elena, David Modrý, and Jirků Pomajbíková, Kateřina
- Format:
- print, počítač, and online zdroj
- Type:
- model:article and TEXT
- Subject:
- šelmy, psi, carnivora, dogs, Česko, Europe, Czechia, vector-borne disease, 2, and 59
- Language:
- English
- Description:
- The eye nematode Thelazia callipaeda Railliet et Henry, 1910 (Spirurida: Thelaziidae) is a vector-borne zoonotic nematode infecting a range of wild and domestic carnivores as well as humans. It is considered to be a causative agent of emerging and neglected disease and currently invades central part of Europe. Nematodes were collected from the eye of a dog living in Prague, which never travelled outside the Czech Republic. The nematodes were identified based on their morphology and partial sequence of the cox1 gene as T. callipaeda haplotype 1. This finding represents the northernmost record of autochthonous canine thelaziosis in Europe. The insufficient control of imported animals as well as free movement of dogs and wild carnivores within Europe probably facilitates spreading of T. callipaeda throughout the continent. To better understand the spreading of T. callipaeda and to prevent its zoonotic transmissions, information about the risk of this infection in newly invaded countries should be disseminated not only among veterinarians and physicians, but also within the community of pet owners and hunters., Milan Jirků, Roman Kuchta, Elena Gricaj, David Modrý and Kateřina Jirků Pomajbíková., and Obsahuje bibliografii
- Rights:
- http://creativecommons.org/publicdomain/mark/1.0/ and policy:public
6. Castosyringophilus meropis sp. n. (Acariformes: Syringophilidae): a new quill mite species parasitising the world population of Merops apiaster Linnaeus (Coraciiformes: Meropidae)
- Creator:
- Skoracki, Maciej, Martin Hromada, and Sikora, Bozena
- Format:
- print, počítač, and online zdroj
- Type:
- model:article and TEXT
- Subject:
- ptáci, parazitologie, birds, parasitology, acari, ectoparasites, faunistics, systematics, 2, and 59
- Language:
- English
- Description:
- A new species, Castosyringophilus meropis sp. n., found in quills of feathers of the European bee-eater Merops apiaster Linnaeus (Coraciiformes: Meropidae) is described. This new species is close to C. claravis Skoracki et Glowska, 2008 and differs, in females, by the presence apunctate coxal fields (vs sparsely punctate in C. claravis) and by the lengths of setae d1 145-180 µm, f2 170-185 µm and ag3 190-215 µm (vs d1 200-220 µm, f2 230-250 µm and ag3 150-170 µm). We present a vast mite material collected from bee-eaters originated from different localities in Europe, Asia and Africa, both breeding and wintering grounds of this bird. It indicates that the whole world population of the European bee-eater is parasitised by this quill mite species., Maciej Skoracki, Martin Hromada, Bozena Sikora., and Obsahuje bibliografii
- Rights:
- http://creativecommons.org/publicdomain/mark/1.0/ and policy:public
7. Ceratomyxa bohari sp. n. (Myxozoa: Ceratomyxidae) from the gall bladder of Lutjanus bohar Forsskål from the Red Sea coast off Saudi Arabia: morphology, seasonality and SSU rDNA sequence
- Creator:
- Mansour, Lamjed, Abdel-Baki, Abdel-Azeem S, Tamihi, Ahmad F, and Al-Quraishy, Saleh
- Format:
- print, počítač, and online zdroj
- Type:
- model:article and TEXT
- Subject:
- parazitické organismy, spóry, fylogeneze, parasitic organisms, spores, phylogeny, Myxosporea, fish parasites, Bivalvulida, coelozoic infection, Lutjanidae, 2, and 59
- Language:
- English
- Description:
- A new myxozoan, Ceratomyxa bohari sp. n., infecting the gall bladder of two-spot red snapper, Lutjanus bohar Forsskål, in the Red Sea off Saudi Arabia, is described using light microscopy and characterised genetically. The infection was recorded as mature spores floating free in the bile. The overall prevalence of infection of the type host was 19% (67 fish infected of 360 examined), with the highest prevalence in autumn (31%; 28/90) and the lowest in winter at 12% (11/90). Mature spores are slender and slightly crescent-shaped in the frontal view, with anterior and posterior margins tapered gradually to rounded valvular tips. Spore valves are unequal with a prominent sutural line. The spore dimensions are 3-4 μm (mean 3.5 μm) in length and 16-19 μm (mean 17 μm) in thickness. Two polar capsules are spherical, equal in size, 1.5 μm in diameter. Coils of the polar filament are indiscernible. The sporoplasm is binucleated and fills nearly one third of the extracapsular space restricted to the area below the capsules. The molecular analysis based on the small subunit rDNA (SSU rDNA) sequence revealed a close relationship with majority of species of Ceratomyxa Thélohan, 1892 and phylogenetic clustering with species from different geographical location. Thus, the shorter spore of the present Ceratomyxa species and the divergence of the SSU rDNA sequences were the distinctive features that separate it from all previously described species and identified this parasite as a new species of Ceratomyxa., Lamjed Mansour, Abdel-Azeem S. Abdel-Baki, Ahmad F. Tamihi, Saleh Al-Quraishy., and Obsahuje bibliografii
- Rights:
- http://creativecommons.org/publicdomain/mark/1.0/ and policy:public
8. Český lid 89/2002
- Format:
- Type:
- model:internalpart and TEXT
- Language:
- Czech
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public
9. Contents
- Format:
- Type:
- model:internalpart and TEXT
- Language:
- Multiple languages
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public
10. Contents
- Format:
- Type:
- model:internalpart and TEXT
- Language:
- Multiple languages
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public
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