Comparison of the dynamics and antigen recognition profiles of IgG3 following immunisation with larval crude extracts (LCE) and excretory-secretory (ES) products from muscle larvae of different species of Trichinella (T. spiralis, T. nativa, T. britovi, T. nelsoni and genotype T6) was made in BALB/c mice. High levels of IgG3 response were obtained in ELISA following immunisation with LCE from all species with maximum levels achieved between days 59 and 64 post-immunisation (p.i.) and maintained up to the limit of the observation (day 164). Antigen recognition profiles as measured by western-blot showed dense and numerous bands in the range 45-64 kDa that were consistent from week 5lh with variation in epitope recognition among the different species. Following immunisation with ES antigens a significant decrease in IgG3 response was observed for all species especially for T. nativa in comparison to LCE. Antigen recognition on ĽS antigens showed three main bands in the range of 45-60 kDa for all species excepting T. nelsoni and T. britovi where an additional band was also present. These results clearly show that IgG3 epitopes are more abundanl in somatic (LCE) than in ES products of Trichinella muscle larvae and that quantitative as well as qualitative variations exist among different species.
A graphical method of estimating bed shear from measured velocity profiles is presented as an alternative to logarithmic law approach. In the present approach the entire velocity profile is considered as per binary law of velocity distribution i.e., logarithmic law in the wall region and parabolic law in the outer region. The validity of this method has been demonstrated for a typical velocity profile. An analysis has been also made in case of an erroneous measurement of bed level. and Práca obsahuje grafickú metódu určenia charakteristík trenia na dne koryta z meraných profilov rýchlosti ako alternatívu k používanému prístupu vychádzajúcemu z logaritmického zákona rozdelenia rýchlostí prúdenia. Predložený prístup charakterizuje profil rýchlosti ako binárny, t.j. zložený z logaritmického rozdelenia v oblasti steny a z parabolického rozdelenia mimo túto oblasť. Vhodnosť tohto prístupu bola demonštrovaná pre typický rýchlostný profil. Bola vykonaná analýza aj pre nepresné meranie úrovne dna koryta.