Neutral trehalase 1 (Nth1) from Saccharomyces cerevisiae
catalyzes disaccharide trehalose hydrolysis and helps yeast to
survive adverse conditions, such as heat shock, starvation or
oxidative stress. 14-3-3 proteins, master regulators of hundreds
of partner proteins, participate in many key cellular processes.
Nth1 is activated by phosphorylation followed by 14-3-3 protein
(Bmh) binding. The activation mechanism is also potentiated by
Ca2+ binding within the EF-hand-like motif. This review
summarizes the current knowledge about trehalases and the
molecular and structural basis of Nth1 activation. The crystal
structure of fully active Nth1 bound to 14-3-3 protein provided
the first high-resolution view of a trehalase from a eukaryotic
organism and showed 14-3-3 proteins as structural modulators
and allosteric effectors of multi-domain binding partners.
The paper deals with mathematical modelling and computer simulation of the seismic response of chosen components of hte nuclear WWER 1999 reactor. The seismic response is investigated by numerical integration method in time domain. The seismic excitation is given by two horizontal and one vertical synthetic accelerograms at the level of the pressure vessel seating adjusted for a certain damping vallue 5 %. The main aim of the paper is seismic response assessment for two variants of nuclear fuel assemblies - VVANTAGE 6 and TVSA-T. The maximal values of displacements and linkages or component deformations are compared for both fuel assembly variants. and Obsahuje seznam literatury