This paper presents an object-oriented two-dimensional (2-D) overland flow model and its application in simulating flood flows over Ulus basin, located in the north of Turkey adjacent to the Black Sea. A new coding implementation according to the class environment created in object oriented C++ programming language is carried out in structuring and building the solver. The model is based on the Godunov type finite volume scheme on unstructured triangular meshes. A mass balance preserving wet/dry boundary solution algorithm is integrated in the numerical scheme to satisfy the positive-depth condition and minimize the numerical instability when treating the propagation of wave front in regions of dry bed. The balance between bed slope and flux terms is also preserved for still water conditions on irregular topography. The 2-D solver is verified by simulating selected dam break cases, where good agreement with measured data is achieved. For the simulation of flood flows in the Ulus basin, in general, the simulated outflow hydrograph is found to compare well with the recorded data. A selected inundation map that is extracted from the model results is also presented to show the water surface level in the Floodplain.
The objective of the paper is to determine the influence of IGF-1
deletion on renal sympathetic nerve activity (RSNA), left
ventricular dysfunction, and renal function in deoxycorticosterone
acetate (DOCA)-salt hypertensive mice. The DOCA-salt
hypertensive mice models were constructed and the experiment
was classified into WT (Wild-type mice) +sham, LID (Liverspecific IGF-1 deficient mice) + sham, WT + DOCA, and LID +
DOCA groups. Enzyme-linked immunosorbent assay (ELISA) was
used to detect the serum IGF-1 levels in mice. The plasma
norepinephrine (NE), urine protein, urea nitrogen and creatinine,
as well as RSNA were measured. Echocardiography was
performed to assess left ventricular dysfunction, and HE staining
to observe the pathological changes in renal tissue of mice.
DOCA-salt induction time-dependently increased the systolic
blood pressure (SBP) of mice, especially in DOCA-salt LID mice.
Besides, the serum IGF-1 levels in WT mice were decreased after
DOCA-salt induction. In addition, the plasma NE concentration
and NE spillover, urinary protein, urea nitrogen, creatinine and
RSNA were remarkably elevated with severe left ventricular
dysfunction, but the creatinine clearance was reduced
in DOCA-salt mice, and these similar changes were obvious in
DOCA-salt mice with IGF-1 deletion. Moreover, the DOCA-salt
mice had tubular ectasia, glomerular fibrosis, interstitial cell
infiltration, and increased arterial wall thickness, and the
DOCA-salt LID mice were more serious in those aspects.
Deletion of IGF-1 may lead to enhanced RSNA in DOCA-salt
hypertensive mice, thereby further aggravating left ventricular
dysfunction and renal damage.
Diabetic nephropathy, included in diabetic kidney disease (DKD), is the primary disease leading to end-stage renal disease (ESRD) or dialysis treatment, accounting for more than 40 % of all patients with ESRD or receiving dialysis. Developing new therapeutics to prevent the transition to ESRD or dialysis treatment requires an understanding of the pathophysiology of DKD and an appropriate animal model for drug efficacy studies. In this study, we investigated the pathophysiology of diabetic kidney disease with type 2 diabetes in uninephrectomized db/db mice. In addition, the nephrectomized db/db mice from 10 weeks to 42 weeks were used to assess the efficacy of longterm administration of the angiotensin-II–receptor antagonist losartan. The blood and urinary biochemical parameters and the blood pressure which is a main pharmacological endpoint of the losartan therapy, were periodically measured. And at the end, histopathological analysis was performed. Uninephrectomized db/db mice clearly developed obesity and hyperglycemia from young age. Furthermore, they showed renal pathophysiological changes, such as increased urinary albumin-creatinine ratio (UACR) (the peak value 3104±986 in 40-week-old mice), glomerular hypertrophy and increased fibrotic areas in the tubulointerstitial tubules. The blood pressure in the losartan group was significantly low compared to the normotensive Vehicle group. However, as expected, Losartan suppressed the increase in UACR (829±500) indicating the medication was sufficient, but the histopathological abnormalities including tubular interstitial fibrosis did not improve. These results suggest that the uninephrectomized db/db mice are useful as an animal model of the severe DKD indicated by the comparison of the efficacy of losartan in this model with the efficacy of losartan in clinical practice.
The need for a better understanding of factors controlling the variability of soil water content (θ) in space and time to adequately predict the movement of water in the soil and in the interphase soil-atmosphere is widely recognised. In this paper, we analyse how soil properties, surface cover and topography influence soil moisture (θ) over karstic lithology in a sub-humid Mediterranean mountain environment. For this analysis we have used 17 months of θ measurements with a high temporal resolution from different positions on a hillslope at the main recharge area of the Campo de Dalías aquifer, in Sierra de Gádor (Almería, SE Spain). Soil properties and surface cover vary depending on the position at the hillslope, and this variability has an important effect on θ. The higher clay content towards the lower position of the hillslope explains the increase of θ downslope at the subsurface horizon throughout the entire period studied. In the surface horizon (0-0.1 m), θ patterns coincide with those found at the subsurface horizon (0.1-0.35 m) during dry periods when the main control is also exerted by the higher percentage of clay that increases downslope and limits water depletion through evaporation. However, in wet periods, the wettest regime is found in the surface horizon at the upper position of the hillslope where plant cover, soil organic matter content, available water, unsaturated hydraulic conductivity (Kunsat) and infiltration rates are higher than in the lower positions. The presence of rock outcrops upslope the θ sampling area, acts as runoff sources, and subsurface flow generation between surface and subsurface horizons also may increase the differences between the upper and the lower positions of the hillslope during wet periods. Both rock and soil cracks and fissures act disconnecting surface water fluxes and reducing run-on to the lower position of the hillslope and thus they affect θ pattern as well as groundwater recharge. Understanding how terrain attributes, ground cover and soil factors interact for controlling θ pattern on karst hillslope is crucial to understand water fluxes in the vadose zone and dominant percolation mechanisms which also contribute to estimate groundwater recharge rates. Therefore, understanding of soil moisture dynamics provides very valuable information for designing rational strategies for the use and management of water resources, which is especially urgent in regions where groundwater supports human consume or key economic activities.