Chromosome numbers for 239 plants from 84 localities in the Czech Republic, Slovakia, Hungary, Germany and Poland are given. Most of the populations were pentaploid (2n = 45), while hexaploid (2n = 54) and tetraploid (2n = 36) populations were rarer. A long marker chromosome was observed in plants from 8 pentaploid populations. Tetraploid plants occurred mainly in Slovakia and Hungary. In the Czech Republic and Germany, most populations were pentaploid. Hexaploid populations (2n = 54) were rare but scattered over the entire study area. The co-occurrence of two different cytotypes was documented at 7 sites. Most tetraploids were fully sexual and only a few tetraploid plants from Poland were apomictic; pentaploid and hexaploid plants were apomictic. Two morphotypes of H. bauhini were distinguished: tetraploid and hexaploid plants from Slovakia and Hungary, and some hexaploid plants from the Czech Republic were assigned to the H. magyaricum group, while tetraploids and hexaploids from the Czech Republic and Poland plus all pentaploids belong to the H. bauhini group.