Ca2+ is an important factor mediating many biotic and abiotic stress responses in plants. In this study, we measured the chlorophyll (Chl) fluorescence of transgenic rice with increased or decreased expression of a calcium-sensing receptor (OsCaS) gene during water deficit caused by polyethylene glycol to prove our hypothesis that increased Ca2+ in combination with increased OsCaS could enhance the drought resistance of transgenic rice. Transcript abundance (evaluated by RT-PCR) was significantly lower in OsCaS antisense line 766 (AS766) than that in the wild type, while the overexpression line 777 (O777) showed four times higher amount than that in the wild type. Chl fluorescence showed that the photochemical quantum yield of PSII in the light increased due to addition of Ca2+ in the O777, but dropped in the AS766. Nonphotochemical quenching increased under stress in both transgenic lines and in the wild type, but less in the O777. Nonregulatory quantum yield of energy dissipation showed no significant change under drought stress. Photochemical quenching was significantly higher in the O777 than those in the AS766 and in the wild type after the Ca2+ treatment. In the absence of stress, the electron transport rate (ETR) was significantly higher in the O777 than in both the AS766 and the wild type. In contrast, the ETR of the wild type and both transgenic lines decreased under drought stress, while the effect of polyethylene glycol was partially alleviated by Ca2+ addition in the O777. In summary, excitation energy conversion and dissipation by PSII were regulated by Ca2+ in the O777. It might partially alleviate the effect of drought stress, whereas addition of Ca2+ had no effect in the wild type and the AS766., R. Wei, Y. Liu, Y. Sui, M. Xu, S. Liu, X. Zhao., and Obsahuje seznam literatury
Trh s učebnými pomôckami poskytuje množstvo rôznych pomôcok, experimentálnych zostáv, ktoré je možné na vyučovaní bez problémov využiť. V mnohých prípadoch si však ich zakúpenie vyžaduje nemalé finančné prostriedky. V súčasnosti existuje veľa možností na vytvorenie prototypu experimentálnej súpravy, ktorá by mala potenciál stať sa užitočnou a najmä lacnou pomôckou vo vzdelávaní mladých ľudí, nie je to však triviálna úloha. Cieľom predkladaného príspevku je ukázať, aké problémy sme objavili (a museli vyriešiť) pri konštrukcii základného prvku nami navrhovanej experimentálnej zostavy - zdroja jednosmerného napätia pre viaceré moduly v experimentálnej súprave., The market for teaching aids provides many different tools and experimental sets suitable for seamless teaching. However, in many cases, their purchase requires considerable funds. At present, there are any ways to create a prototype of an experimental set, which has the potential to become a useful and cheap tool in the education of young people, but this is not a trivial task. In this article, we introduce several problems which were discovered and solved during the construction of a basic part of a DC power supply suitable for individual experimental modules., Martin Hruška., and Obsahuje bibliografické odkazy