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Start Over Coverage 525-530

2. GFP transgenic animals in biomedical research: a review of potential disadvantages

Creator:
Lipták, N. , Bősze, Z., and Hiripi, L.
Format:
bez média and svazek
Type:
model:article and TEXT
Subject:
GFP, transgenic animals, glomerulosclerosis, and dilated cardiomyopathy
Language:
English
Description:
Green Fluorescent protein (GFP) transgenic animals are accepted tools for studying various physiological processes, including organ development and cell migration. However, several in vivo studies claimed that GFP may impair transgenic animals’ health. Glomerulosclerosis was observed in transgenic mice and rabbits with ubiquitous reporter protein expression. Heart-specific GFP expression evoked dilated cardiomyopathy and altered cardiac function in transgenic mouse and zebrafish lines, respectively. Moreover, growth retardation and increased axon swelling were observed in GFP and yellow fluorescent protein (YFP) transgenic mice, respectively. This review will focus on the potential drawbacks of the applications of GFP transgenic animals in biomedical research.
Rights:
http://creativecommons.org/publicdomain/mark/1.0/ and policy:public

3. Isolation and characterization of photosystem 2 PsbR gene and its promoter from drought-tolerant plant Prosopis juliflora

Creator:
Suja, G. and Parida, A.
Format:
bez média and svazek
Type:
model:article and TEXT
Subject:
H2O2, Northern analysis, photosystem 2, polyethylene glycol, proteins, transcript level, and water deficit
Language:
Multiple languages
Description:
Prosopis juliflora is a hardy plant tolerant to drought, salinity, extremes of soil pH, and heavy metal stress. We isolated and characterized a photosystem 2 (PS2) gene PsbR (Pj PsbR) and its promoter. Northern analysis for Pj PsbR in P. juliflora leaves under 25 % polyethylene glycol stress showed steady decrease in transcript level at 12, 24, and 48 h after stress application. Under 90 mM H2O2 stress, transcript level dropped drastically at 12 h, but increased again compared to the control at 24 h. A 1.7 kb fragment upstream the 5' UTR of this gene (putative promoter) was isolated and analyzed in silico. Several putative cis-acting DNA elements were identified in this sequence. and G. Suja, A. Parida.
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public