The most recent genome-editing system called CRISPR-Cas9 (clustered regularly interspaced short palindromic repeat system with associated protein 9-nuclease) was employed to delete four non-essential genes (i.e., Caeco1, Caidh1, Carom2, and Cataf10) individually to establish their gene functionality annotations in pathogen Candida albicans. The biological roles of these genes were investigated with respect to the cell wall integrity and biogenesis, calcium/calcineurin pathways, susceptibility of mutants towards temperature, drugs and salts. All the mutants showed increased vulnerability compared to the wild-type background strain towards the cell wall-perturbing agents, (antifungal) drugs and salts. All the mutants also exhibited repressed and defective hyphal growth and smaller colony size than control CA14. The cell cycle of all the mutants decreased enormously except for those with Carom2 deletion. The budding index and budding size also increased for all mutants with altered bud shape. The disposition of the mutants towards cell wall-perturbing enzymes disclosed lower survival and more rapid cell wall lysis events than in wild types. The pathogenicity and virulence of the mutants was checked by adhesion assay, and strains lacking rom2 and eco1 were found to possess the least adhesion capacity, which is synonymous to their decreased pathogenicity and virulence.
In this paper we study $J$-EP matrices, as a generalization of EP-matrices in indefinite inner product spaces, with respect to indefinite matrix product. We give some properties concerning EP and $J$-EP matrices and find connection between them. Also, we present some results for reverse order law for Moore-Penrose inverse in indefinite setting. Finally, we deal with the star partial ordering and improve some results given in the “EP matrices in indefinite inner product spaces” (2012), by relaxing some conditions.
The main result of this paper is the introduction of a notion of a generalized RLatin square, which includes as a special case the standard Latin square, as well as the magic square, and also the double stochastic matrix. Further, the algebra of all generalized Latin squares over a commutative ring with identity is investigated. Moreover, some remarkable examples are added.