The relationships of 14 Central European species of the genus Micrasterias were analysed using landmark-based geometric morphometrics. The analysis of relative warps was used to depict the principal components of the variation in shape and cluster analysis to reveal the groupings of individual species within the genus. All the analysed cells were correctly placed in their appropriate species clusters on the basis of geometric morphometric data. The width of the polar lobe associated with depth of the incisions between lateral lobules is the dominant morphological trend in the data investigated.