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Start Over Creator Blanka Železná Creator Jaroslav Kuneš Subject 612 Type model:article

1. Influence of active immunization against angiotensin AT1 or AT2 receptor on hypertension development in young and adult SHR

Creator:
Blanka Železná, Leopold Veselský, Jiří Velek, Zdena Dobešová, Josef Zicha, and Jaroslav Kuneš
Format:
print, bez média, and svazek
Type:
article, články, model:article, and TEXT
Subject:
Fyziologie člověka a srovnávací fyziologie, krevní tlak, hypertrofie srdce, blood pressure, heart hypertrophy, spontaneously hypertensive rats, active immunization, angiotensin AT1 receptor, angiotensin AT2 receptor, kidney weight, 14, and 612
Language:
English
Description:
B. Železná, L. Veselský, J. Velek, Z. Dobešová, J. Zicha, J. Kuneš. and Obsahuje bibliografii
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public

2. Potential neuroprotective and anti-apoptotic properties of a long-lasting stable analog of ghrelin: an in vitro study using SH-SY5Y cells

Creator:
Alena Popelová, Kákonová, A, Blanka Železná, Lenka Maletínská, Jaroslav Kuneš, and Hrubá, L
Format:
print, bez média, and svazek
Type:
model:article and TEXT
Subject:
fyziologie člověka, human physiology, Ghrelin, SH-SY5Y cells, Methylglyoxal cytotoxicity, Neuroprotection, Apoptosis, 14, and 612
Language:
English
Description:
Neurodegenerative disorders, such as Alzheimer’s disease (AD) and Parkinson’s disease (PD), are increasing in prevalence. Currently, there are no effective and specific treatments for these disorders. Recently, positive effects of the orexigenic hormone ghrelin on memory and learning were demonstrated in mouse models of AD and PD. In this study, we tested the potential neuroprotective properties of a stable and long-lasting ghrelin analog, Dpr3ghrelin (Dpr3ghr), in SH-SY5Y neuroblastoma cells stressed with 1.2 mM methylglyoxal (MG), a toxic endogenous by-product of glycolysis, and we examined the impact of Dpr3ghr on apoptosis. Pre-treatment with both 10-5 and 10-7 M Dpr3ghr resulted in increased viability in SH-SY5Y cells (determined by MTT staining), as well as reduced cytotoxicity of MG in these cells (determined by LDH assay). Dpr3ghr increased viability by altering pro-apoptotic and viability markers: Bax was decreased, Bcl-2 was increased, and the Bax/Bcl-2 ratio was attenuated. The ghrelin receptor GHS-R1 and Dpr3ghr-induced activation of PBK/Akt were immuno-detected in SH-SY5Y cells to demonstrate the presence of GHS-R1 and GHS-R1 activation, respectively. We demonstrated that Dpr3ghr protected SH-SY5Y cells against MG-induced neurotoxicity and apoptosis. Our data suggest that stable ghrelin analogs may be candidates for the effective treatment of neurodegenerative disorders., A. Popelová, A. Kákonová, L. Hrubá, J. Kuneš, L. Maletínská, B. Železná., and Seznam literatury
Rights:
http://creativecommons.org/publicdomain/mark/1.0/ and policy:public