Search

Start Over Creator Ditrich, Oleg Language English Type model:internalpart Original context has metadata only false

2. Cytokine response to infection with the microsporidian, Encephalitozoon cuniculi

Creator:
Braunfuchsová, Pavlína, Kopecký, Jan, Ditrich, Oleg, and Koudela, Břetislav
Format:
print
Type:
model:internalpart and TEXT
Subject:
microsporidia, Encephalitozoon, cytokines, and SCID mice
Language:
English
Description:
The production of three cytokines, interferon gamma (IFN-y), interleukin 10 (1L-10) and interleukin 12 (IL-12), was measured after intraperitoneal infection of immunocompetent Balb/c mice and immunodeficient SCID mice with the microsporidian, Encephalitozoon cuniculi Levaditi, Nicolau ct Schoen, 1923. High levels of IFN-y were detected in ex vivo cultures of peritoneal exudate cells (PEC) of Balb/c mice, a lower, but earlier IFN-y response was observed in PEC from SCID mice. The early 1L-10 response was detected in ex vivo cultures of splenocytes from Balb/c but not from SCID mice, explaining a delay in the IFN-y response in Balb/c mice. IL-12 was detected in PEC cultures from SCID mice, indicating an alternative pathway of IFN-y production by NK. cells stimulated by IL-12 derived from macrophages.
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public

4. Larval stages of trematodes from freshwater molluscs of the Yucatan Peninsula, Mexico

Creator:
Ditrich, Oleg, Scholz, Tomáš, Aguirre-Macedo, Leopoldina, and Vargas-Vázquez, Joaquin
Format:
print
Type:
model:internalpart and TEXT
Subject:
freshwater molluscs, Pyrgophorus coronatus, Trematoda, cercariae, Genarchella astyanactis, life-cycles, Yucatan, and Mexico
Language:
English
Description:
Examination of 4055 molluscs of 10 species from cenotes (= sinkholes) and other freshwater bodies in the Yucatan Peninsula, Mexico revealed the presence of-larval stages of 13 trematodes. The following species were found: Echinochasmus leopoldinae Scholz, Ditrich et Vargas-Vázquez, 1996, E. macrocaudatus Ditrich, Scholz et Vargas-Vázquez, 1996 (Echi-nostomatidae), Saccocoelioides sp. (? sogandaresi Lumsden, 1963) (Haploporidae), Crassicutis cichlasomae Manter, 1936, pleurolophocercous ophthalmocercaria sp. (Homalometridae), Ascocotyle (Ascocotyle) sp., Ascocotyle (Phagicola) nana Ransom, 1920 (Heterophyidae), Oligogonotylus manieri Watson, 1976 (Cryptogonimidae), Genarchella astyanactis (Watson, 1976) (Derogenidae), xiphidiocercariae sp. 1, 2 and 3 (Lecithodendriidae?), and furcocercaria gen. sp. (Fellodistomidae). The life-cycle of the derogenid Genarchella astyanactis was studied for the first time. It was found that it differs from that of G. genarchella: the first intermediate host, Pyrgophorus coronatus (Pfeiffer, 1839), released cystophorous furcocercariae of G. astyanactis that developed, after ingestion by the second intermediate host, copepods (experimentally Mesocyclops chaci Fiers, Reid, Ilife et Suárez-Morales, 1996), into metacercariae resembling by their morphology juvenile trematodes found in the stomach of Aslya-nax fasciatus. No progenetic cercariae (metacercariae) found in G. genarchella were observed in the life-cycle of G. astyanactis. Rediae and cystophorous furcocercariae were recovered from naturally infected snails and snails experimentally kept in contact with eggs from the uterus of G. astyanactis adults.
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public

5. The serological surveillance of several groups of patients using antigens of Encephalitozoon hellem and E. cuniculi antibodies to microsporidia in patients

Creator:
Kučerová-Pospíšilová, Zuzana and Ditrich, Oleg
Format:
print
Type:
model:internalpart and TEXT
Subject:
microsporidia, Encephalitozoon cuniculi, Encephalitozoon hellem, and antimicrosporidial IgG antibodies
Language:
English
Description:
This study was undertaken to attempt to identify correlations between microsporidial seroprevalence data in man, clinical diseases and groups of people at the risk of HIV/AIDS infection. Groups of patients were selected according to the predilection of members of the genus Encephalitozoon for nervous and kidney tissue. Female prostitutes and alcohol and intravenous drug abusers were selected as groups at risk of HIV/AIDS infections. A total of 401 samples of human sera were examined for the presence of antimicrosporidial IgG antibodies by ELISA test with a titre of 600 considered borderline positivity. The highest occurrence of antimicrosporidial antibodies was found in the groups of alcohol abusers (16 % from 43 patients), intravenous drug abusers (11 % from 9 patients) and prostitutes (10 % from 80 women) for E. cuniculi antigen and in the groups of psychiatric patients (14 % from 44 patients), malaria patients (11 % from 38 patients) and alcohol abusers (7 % from 43 patients) for E. hellem antigen. The occurrence of specific antibodies of the six examined diagnostic units (glomerulonephritis chronica, pyelonephritis chronica, schizophrenia, dementia, multiple sclerosis and cerebral stroke) was statistically significant only in patients with pyelonephritis chronica and dementia (p < 0.05), No cases of microsporidial infection were found among the female prostitutes by parasitological examination, although one case of giardiasis was identified. Sera of patients with high anti-/:, cuniculi and anti-fc'. hellem antibodies (titres in ELISA of 600 and above) were confirmed by Western blot using E. cuniculi and E. hellem polypeptides, respectively. These results suggest that the examined patients could show residual antibodies from past or latent infections.
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public