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1. Nitric oxide in the periendothelial area of femoral vein of the dog assessed in vivo by a porphyrinic sensor

Creator:
Gerová, M., Mesároš, Š., Kittová, M., Hatrik, Š., Kristek, F., and Malinski, T.
Type:
article, model:article, and TEXT
Subject:
nitric oxide, venous endothelium, and electrochemical biosensor
Language:
English
Description:
Nitric oxide concentration in the periendothelial area of the femoral vein in anaesthetized dogs was measured directly with a catheter- protected porphyrinic sensor. A 2- to 4-fold increase occurred in the basal NO concentration of 90±12 nM after acetylcholine injection (1-1.5 ,wg/kg). A linear correlation was found between femoral artery blood flow and NO concentration in the periendothelial area of the femoral vein. Noradrenaline decreased NO levels below the detection limit of the porphyrinic sensor (10 nM).
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public

2. NO concentration in the periendothelial area of the femoral artery of the dog measured in vivo

Creator:
Gerová, M., Mesaroš, Š., Kristek, F., Kittová, M., and Malinski, T.
Type:
article, model:article, and TEXT
Subject:
arterial • NO concentration, A-V difference in • NO, and • NO synthase inhibition
Language:
English
Description:
• NO concentration was measured in the periendothelial area of the femoral artery by Malinski’s porphyrinic • NO sensor in seven anaesthetized dogs. The basal concentration was 154.2 ±5.6 nM and two-minute intraarterial infusions of acetylcholine (3-4 /tg/ml/min) or bradykinin (30 - 40 ng/ml/min) increased this value significantly to 204.3±16.4 and 266.5±16.4 nM (P<0.01), respectively. Inhibition of »NO synthase by L-NAME (50 mg/kg) declined the basal • NO concentration only to 137.2±3.3 nM (PcO.Ol). Subsequent administration of acetylcholine and bradykinin attenuated significantly the increase in • NO concentration. Surprisingly, both agonists still induced a significant increase of *NO concentration by 125.3±8.3 and 156.6±26.9 nM, respectively (PcO.Ol). One of the possible explanations may be that besides arginine-citrulline plus the • NO pathway other sources of • NO could be involved in the high level of • NO after • NO synthase blockade by L-NAME.
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public