Two species of the North American genus Oenothera are reported as aliens in the Czech Republic. A population of O. coronifera consisting of ca. 30 plants at various phenological stages, from rosettes to flowering plants, was found in 2001 at the railway station in the town of Zliv, district of České Budějovice, S Bohemia. The species was probably introduced via the railway and is the first record of this species for the Czech Republic. A single plant of O. stricta, previously reported from the bank of the Vltava river in Prague, in 1825, was found as a weed in a private garden in the village of Vroutek, district of Louny, N Bohemia, in 2000. This is the second record of this species from the Czech Republic in 175 years. The seed of O. stricta was probably introduced to the site from abroad and the record suggests that the occurrence of casual alien plants is highly unpredictable. It is argued that botanists studying alien plants, given their special interest in sites where these plants occur, may directly contribute to the enrichment of checklists of national alien floras.