Quercetin, rutin and phioridzin inhibited the activity of glyceraldehyde-3-phosphate dehydrogenase (GPD) complex from spinách leaves. At a concentiation of 5 X 10'5 M they decreased the rate of 3-phosphoglycerate (PGA) leduction by 90, 50- 80 and 30-40 % , respectively. The activity of the effectors depended on the tiine of isolation of enzymes and the corresponding level of endogenic phenolic compounds. To estimate the effect of the last factor, the enzj^e preparation was obtained in the presence and in the absence of insoluble polyvinylpyrrolidone (Polyciar AT). Using rutin it was shown that in the absence of endogenic phenols the inhibition of PGA reduction was decreased as compared with the variant without Polyciar AT. The effect of rutin was constant during the day. On the basis of data obtained the flavonoids are supposed to inhibit the Benson-Calvin cycie at its reducing stage; this effect may be cooperative and depend on quantitative and qualitative content of endogenic phenolic compounds.