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Start Over Creator Vácha, Martin

2. Světélkující molekuly a mikroskopie za hranicí svých možností: Nobelova cena za chemii 2014

Creator:
Vácha, Martin
Format:
print, bez média, and svazek
Type:
model:article and TEXT
Subject:
chemie, chemistry, 6, and 53
Language:
Czech
Description:
The Nobel Prize for Chemistry in 2014 was awarded for the development of super resolved fluorescence microscopic methods. This contribution introduces the two approaches, which overcome the diffraction limit of optical resolution, namely single molecule localization microscopy and spatially selective inhibition of fluorescence. The development of single molecule localization is followed from a historical perspective from its origins as low temperature single molecule spectroscopy to room temperature spectroscopy and imaging, which eventually led to methods of photoactivated fluorescence microscopy (PALM), stochastic reconstruction microscopy (STORM) and other high resolution techniques. The approach of spatial inhibition of fluorescence is demonstrated mainly by stimulated emission depletion (STED) techniques., Martin Vácha., and Obsahuje seznam literatury
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public