Prolonged agonist stimulation results in specific transfer of activated Gα subunits of Gqα/G11α family from particulate membrane fraction to soluble (cytosol) cell fraction isolated as 250 000 x g supernatant. In this study, we have used 2D electrophoresis for more defined resolution of Gα subunits of Gqα/G11α family and followed the time course of solubilization effect. The small signal of soluble G proteins was already detected in control, hormone-unexposed cells. Hormone stimulation resulted in a slow but continuous increase of both intensity and number of immunoreactive signals/spots of these G proteins (10, 30, 60, 120 and 240 min). At longer times of agonist exposure (>2 hours), a marked increase of Gqα/G11α proteins was detected. The maximal level of soluble Gqα/G11α proteins was reached after 16 hours of continuous agonist exposure. At this time interval, eight individual immunoreactive signals of Gqα/G1 α proteins could be resolved. The relative proportion among these spots was 15:42:10:11:7:7:2:5. Solubilization of this class of Gα proteins was thus observed after prolonged agonist stimulation only, induced by ultra high concentration of hormone and in cells expressing a large number of GPCRs. Our data therefore rather indicate tight/persisting binding of Gqα/G11α proteins to the membrane., D. Durchánková, J. Novotný, P. Svoboda., and Obsahuje bibliografii a bibliografické odkazy
Chemické vizualizace a modely jsou zvláštními druhy médií myšlení. Tato studie zkoumá několik historických případových studií – archív obrazů z muzeí, specializovaných sbírek a populárně-vědeckých časopisů – emergentních praktik materiálního modelování coby teoretické hry, jež se staly základem molekulární biologie a strukturní chemie. Sleduji dědictví nástrojů vizualizace počínaje Archibaldem Scottem Cooperem a Friedrichem Kekulé na konci 19. století, jejich vyústění do materiálních manipulativů Van’t Hoffa a užití jeho skládaných papírových "hraček“ Linusem Paulingem i jejich následné pronikání do populární obraznosti díky modelu DNA Jamese Watsona a Francise Cricka. Sleduji dále jejich vliv na současné praktiky modelování a zdůrazňuji, že materiální modely, jež tradičně stály za hranicemi deduktivní, pozitivistické vědy, jsou nyní v těchto oblastech chemie akceptovány jako způsob vědeckého uvažování., Chemical visualizations and models are special kinds of media for thinking. In this paper, I examine several historical case studies-an archive of images from museums, special collections, and popular magazines- as examples of emergent practices of physical modeling as theoretical play which became the basis for molecular biology and structural chemistry. I trace a legacy of visualization tools that starts with Archibald Scott Cooper and Friedrich Kekulé in the late 1800s, crystallizes as material manipulatives in Van’t Hoff and his folded paper “toys”, is legitimized in the California lab of Linus Pauling, and is glorified in the popular imaginary with James Watson and Francis Crick’s model of DNA. My tracing then follows several threads into contemporary modeling practices. I ultimately argue that modeling play, originally outside of the boundary of deductive, positivist science, is now an accepted mode of reasoning in these related chemical fields., and Kate McKinney Maddalena.
Studie Veroniky M. Mráčkové se zabývá problematikou přejímání středověkých hymnických melodií, ilustrovanou na konkrétním zvoleném příkladu., The hymn, one of the most frequently encountered songs in Western European sources, poses many questions with regard to the conflict between common and local traits. The Office hymns on which I focus were transmitted mainly in fourteenth- and fifteenth-century sources, which contain huge collections of hymns for different liturgical occasions, including those in honour of local saints. We still do not have a reasonable explanation of how or why the same tunes were adapted for different texts (or vice versa). The ‘Franciscan’ hymn melody Stäblein 752 (originally devoted to St Francis), for example, which occurs with different hymn texts in Austria, Germany, Bohemia, Poland and Spain, has many melodic variants reflecting regional characteristics. Why was this particular melody transferred to and adopted in other parts of Europe, where there was certainly no shortage of alternative hymn melodies? Is it a question of the adoption of favourite melodies in the Middle Ages? Were the same tunes used for hymns in honour of both male and female saints? What are the implications for us when the same ‘local’ tune can be identified in polyphonic hymn settings?, Veronika M. Mráčková., Rubrika: Studie, and České resumé na s. 32, anglický abstrakt na s. 19.
A strongly recommended conclusion in sociology about trends in class inequality has been summarised by Goldthorpe as a high degree of 'temporal constancy and cross-national communality'. This conclusion, here called 'the stability thesis', was first challenged by Ringen in 1987 and again, on more methodological grounds, by Ringen and Hellevik in two papers published in 1997. These challenges resulted in a process of debate and reassessment. It is now possible to sum up and conclude. The stability thesis rests on empirical results from odds-ratio readings of mobility table data. The authority of this methodology is re-examined in terms of normative significance and statistical validity. Mobility table data which have generated stability thesis findings are reanalysed with the standard gini-index methodology in the study of inequality, then yielding different findings which contradict the stability thesis. The main conclusion is that the stability thesis can now be considered overturned. Keywords: social inequality, social justice, social reform, class analysis, social stratification.
Od začátku 21. století roste vědomí, že software je slepou skvrnou teorie nových médií. Vzrůstající zájem o software ovlivnil také tezi předkládaného příspěvku. Vychází z předpokladu, že koncept univerzálního stroje Alana M. Turinga je jedním z mládeneckých strojů (Carrouges). Předchozí texty založené na podobné hypotéze (Daniels, Baudrillard, Turkle, Ascott) se zaměřily buď na srovnání univerzálního stroje a mládeneckého stroje na základějejich strukturálních podobností, nebo užívaly mládenecký stroj jako metaforu člověka nebo počítače (umělé inteligence). Na rozdíl od nich, tento příspěvek zdůrazňuje význam kontextu (imitační hry Turingova testu), který je klíčem k interpretaci univerzálního Turingova stroje jako mládeneckého stroje a potenciálně jako autoportrétu., Since the beginning of the twenty-first century there has been an increasing awareness that software represents a blind spot in new media theory. the growing interest in software also influences the argument in this paper, which sets out from the assumption that Alan M. Turing’s concept of the universal machine, the first theoretical description of a computer program (software), is a kind of bachelor machine (Carrouges). Previous writings based on a similar hypothesis (Daniels, Baudrillard, Turkle, Ascott) have focused either on a comparison of the universal machine and the bachelor machine in terms of the similarities of their structural features, or they have taken the bachelor machine as a metaphor for a man or a computer (artificial intelligence). Unlike them, this paper stresses the importance of the context (the imitation game of the Turing test) as a key to interpreting the universal Turing machine as a bachelor machine and, potentially, as a self-portrait., and Jana Horáková.
The anterior jejunum from common vole naturally infected with Giardia microti (Kofoid et Christiansen, 1915) was examined by ТЕМ and compared with the anterior jejunum from control (metronidazole-treated, Giardia-free) common voles (Pallas, 1778). Giardia microti infection resulted in significant diffuse shortening of microvilli and significant greater microvillous diameters. In addition, deformations of the microvilli were observed at the margin of the ventral disc. The microvilli attached to the lateral crest of the ventral disc were vesiculated with a disorganised filamentous core and contained whorled structures resembling “myelin-like figures”. The findings are discussed in context of the Giardia-epithelial cell interaction.
The ultrastructure and histochemistry of the tegument and penetration glands of adult Amphilina foliacea from the body cavity and the tissues of the internal organs of Acipenser ruthenus and A. stellatus were studied. New data on the localization in the tissue, development and in encapsulation of the adult A. foliacea mostly in the liver of A. ruthenus were obtained. The well developed penetration glands are necessary for penetration into the tissue and for migration of A. foliacea into the body cavity of the hosts. The tegument of the adult A. foliacea is a syncytium with cytons deeply embedded into the parenchyma. The secretory activity of the tegument of worms has a protective function against the immune system of the host. Our results give further information about the phylogeny of Amphilinidea and confirm the view of the close phylogenetic relationship of Amphilinidea and Cestoidea.
This paper presents the results of investigations on changes of the parameters of coal and surrounding rocks in the fault zones in the Upper Silesia Coal Basin. It has been shown that these zones, in relation to the undisturbed coal seams, reveal a n enrichment with some substances that pose a threat to the natural environment and that they also show a deterioration in the strength parameter values of rocks which threaten the safety of the operation. The greatest threat for the environment constituted sulphur and the ash. In the zone of the fault, it wa s observed that sulphur behaved in two ways. In the first case, the total sulphur and pyritic sulphur increased significantly by 192.3 and 823.5 %, respectively, and a small quantity of sulphate sulphur was found here, whereas in the other case, the total sulphur and pyritic sulphur decreased by 31.6 an d 35.3 %, respectively, and sulp hate sulphur increased considerably and reached up to 600 %. The mean content of ash increased by 171.8 %. The mechanical strenght of the rocks under uniaxial compressive strength in the fault zones decreased by 67 %, the microhardness of coal decreased to zero, and the intensity of cracks in coal grains increased by 359.1 %., Stanisław Roman Cmiel., and Obsahuje bibliografické odkazy