We studied a natural infection of the oligochaete Branchiura sowerbyi Beddard, 1892 with the Raabeia-type actinosporean stage of Myxobolus lentisuturalis Dyková, Fiala et Nie, 2002 which infected goldfish Carassius auratus auratus (L.) in Italy, using molecular analysis of the SSU rRNA gene. The existence of intraoligochaete development shows that this parasite follows the life-cycle pattern described by Wolf and Markiw (1984) for Myxobolus cerebralis. Histological examinations of the goldfish infected by M. lentisuturalis showed at low magnification the presence of two bilateral crescent-shaped masses in the dorsal epaxial muscle. These lesions were not circumscribed, presented irregular edges and infiltrated the underlying bundles of skeletal muscle and interstitial tissue. At higher magnification, disappearance of muscle fibres and substitution of the muscle tissue with Myxobolus spores and plasmodia were observed.
This article evaluates once more the historiographic and literary images of John of Bohemia and his son Charles IV in Italian texts from the 14th and early 15th centuries. What we find is a peculiar mixture of criticism and apotheosis, sometimes stated by the same authors, depending on the point in time they were writing, and of course the expectations of their potential readers. While John of Bohemia faced overwhelming expectations from Dante after the death of his father, he was branded a naïve yet greedy papal mercenary from the beginning of his Italian Expedition in the early 1330s. His son was more successful in avoiding negative stereotypes and harsh criticisms during his Italian expeditions in his youth, as well as in 1354/55 and 1368/69. In the end, however, even chroniclers that are traditionally considered to have had a positive view of the Luxemburg king and emperor harshly rejected his political actions in Italy. Most of the time, this is connected with the financial interests all foreign monarchs had when establishing temporary rulerships in Italian cities, and the monetary pressures this bore on their citizens; the worn-out cliché, both of contemporaries and historical researchers, that labelled foreign, Central European monarchs as barbaric intruders, could hardly be confirmed. Charles and his father are blamed for being unable to solve the structural problems of Italian and Imperial politics.
The genus Gobio in Italy was represented by the endemic species G. benacensis. The original distribution of this species was the Padano-Venetian district, but since a long time it was introduced in central Italy. Introductions of alien species to Italy during the last 10 years brought the sudden introduction of the Danubian G. gobio. Genetic and morphological analyses revealed the extensive presence of G. gobio, which rapidly colonised several rivers in Italy causing the progressive decline of G. benacensis, which now should be considered as an endangered species. Among examined populations those found in the Tagliamento River and transplanted in the Ombrone River represent genetic reservoirs of this species which will probably disappear in northern Italy.