It is important to determine and clarify the variability of mammary carcinogenesis induction in animal experimental studies particularly in connection with chemoprevention projects. The circannual seasonal rhythms of hormone levels or various parameters within the immune system may involve factors participating in mammary gland carcinogenesis. In our study, 19 experiments were conducted and all of them lasted for about 25 weeks after chemical carcinogen administration (DMBA or NMU) under standard laboratory conditions. Females of two rat strains - a medium susceptible Sprague-Dawley strain and a very low susceptible Wistar:Han were used. We observed not only the effect of seasonal changes but also the effect of age after single or repeated carcinogen administration. The seasonal dependence of mammary carcinogenesis with higher tumor incidence during long days in comparison with winter short days has been demonstrated in Sprague-Dawley rats. In experiments on the Wistar:Han strain, certain features of seasonal character were recorded, although the very low susceptibility of this strain to mammary carcinogenesis might have influenced the results. A limited period of carcinogen administration in early puberty around postnatal days 43-46 (higher susceptibility), when compared to the period after postnatal day 50, is the factor significantly increasing incidence and frequency of mammary carcinogenesis in the Sprague-Dawley strain. Our results indicate the need to consider the effect of season and age of animals at the time of carcinogen administration on rat mammary carcinogenesis induction. However, the application of the results obtained in one strain of experimental animals may only lead to misleading conclusions., P. Kubatka, E. Ahlersová, I. Ahlers, B. Bojková, K. Kalická, E. Adámeková, M. Marková, M. Chamilová, M. Čermáková., and Obsahuje bibliografii
Visual cognitive responses (P300) to moving stimuli were tested in 36 subjects with the aim to find the normal range of P300 parameters. Concomitantly, the circadian intra-individual variability of the P300 was studied in a subgroup of 6 subjects. Visual stimuli consisted of either coherent (frequent stimulus) or non-coherent motion (random stimulus). The oddball paradigm was applied for recording cognitive responses. P300 to rare stimuli had an average latency of 447.3±46.6 ms and amplitude of 12.9±6.0 mV. The average reaction time was in the range from 322 to 611 ms and there was no correlation between the reaction time and P300 latency. We did not find any significant circadian changes of the P300 parameters in the 6 subjects tested four times during the same day. Cognitive (event-related) responses (P300) displayed distinctly greater inter-individual variability (S.D. of 50 ms) when compared with pattern-reversal and motion-onset VEPs (S.D. of 6.0 ms and 14 ms, respectively). For this reason, the clinical use of P300 elicited by this kind of visual stimuli seems to be rather restricted and the evaluation of its intra-individual changes is preferable., Z. Kubová, J. Kremláček, J. Szanyi, J. Chlubnová, M. Kuba., and Obsahuje bibliografii
The relationship between vitamin D receptor (VDR) intragenic polymorphisms FokI, BsmI, ApaI and TaqI and bone mineral density (BMD) or biochemical markers of bone remodeling were investigated in 114 Czech postmenopausal women, on the average 62.5±8.9 years of age. Restriction fragment length polymorphisms in the VDR gene were assessed by PCR amplification and digestion with restriction enzymes FokI, BsmI, ApaI, and TaqI recognizing polymorphic sites in the VDR locus. Bone mineral density was measured at the lumbar spine and at the hip by dual-energy X-ray absorptiometry (DEXA, g/cm2). After adjusting for age and the body mass index (BMI), subjects with the ff genotype had 9.4 % lower BMD at the hip than those with the Ff genotype (p=0.0459, Tukey´s test). FF individuals had an intermediate BMD at the hip. A similar pattern of lower lumbar spine BMD was also found in ff individuals, but it did not reach statistical significance. There was no relationship between BsmI, ApaI and TaqI VDR polymorphisms and BMD at any skeletal site. Subjects with Aa (ApaI) genotypes had higher levels of propeptide of type I collagen (PICP) than homozygous AA (p=0.0459, Tukey´s test). In FokI, BsmI and TaqI restriction sites the biochemical markers of bone remodeling did not differ by genotype. In addition, no significant difference was observed in VDR genotypic distribution between osteoporotic women and non-osteoporotic controls in the study group. To conclude, the FokI genotype of the vitamin D receptor gene is related to bone mass at the hip in Czech postmenopausal women, whereas the importance of remaining VDR genotypes was not evident., K. Zajíčková, I. Žofková, R. Bahbouh, A. Křepelová., and Obsahuje bibliografii
Studie shrnuje, porovnává a kriticky zhodnocuje koncepty, modely a teorie zabývající se vtahováním dětí do rodičovských konfliktů. Zabývá se modely, které vzešly z praxe systemických terapeutů, jako např. triangulace, perverzní koalice nebo aliance. Dále se věnuje konceptům, které vznikly v rámci studia rozvodových konfliktů jako je syndrom zavrženého rodiče a odcizený rodič. Cílem této studie je představit komplexní, jasný a ucelený popis, který by charakterizoval principy vtahování dětí do konfliktů jak v úplných, tak rozvedených rodinách., The review summarizes, compares, and critically evaluates theoretical constructs, which deal with the inclusion of children into the interparental conflict. The authors review constructs based on the work of systemic therapist, such as triangulation, alliance, perverse coalition, and alliance, and psychosomatization. Either, they review theories based on studies of divorced families, such as parental alienation syndrome, and parental alienation. The goal of this study is to introduce enough complex, clear, and comprehensive description, which embrace the principles of inclusion of children into interparental conflicts, both in non-divorced and divorced families., Olga Trampotová, Lenka Lacinová., and Obsahuje seznam literatury
The article outlines the book culture of the Rudolphine period on the examples of several works by Tycho Brahe (Instruments of the Renewed Astronomy), Johannes Kepler (Somnium: The Dream, or Posthumous Work on Lunar Astronomy; Conversation with the Starry Messenger) and Galileo Galilei (The Starry Messenger). It is based on both research outcomes that have already been published and those that are being prepared for printing. and Alena Hadravová.
Studie Klause Dögeho obsahuje analytické poznámky ke kompozici hudebního skladatele Bohuslava Martinů, věnovanému památce vyhlazení Lidic., In his Memorial to Lidice (composed in the summer of 1943) Martinů pays tribute to the complete extinction of the Czech village Lidice and its inhabitants by the Nazis. In the composition he uses – as he has never done before in this way – many traditional idioms and quotation-like elements in order to express all the sadness, fright and pain of his subject., Klaus Döge., Rubrika: Studie, and Německé resumé na s. 18, anglický abstrakt 5.