Study of bacterial transport and retention in soil is important for various environmental applications such as groundwater contamination and bioremediation of soil and water. The main objective of this research was to quantitatively assess bacterial transport and deposition under saturated conditions in calcareous soil. A series of leaching experiments was conducted on two undisturbed soil columns. Breakthrough curves of Pseudomonas fluorescens and Cl were measured. After the leaching experiment, spatial distribution of bacteria retention in the soil columns was determined. The HYDRUS-1D one- and two-site kinetic models were used to predict the transport and deposition of bacteria in soil. The results indicated that the two-site model fits the observed data better than one-site kinetic model. Bacteria interaction with the soil of kinetic site 1 revealed relatively fast attachment and slow detachment, whereas attachment to and detachment of bacteria from kinetic site 2 was fast. Fast attachment and slow detachment of site 1 can be attributed to soil calcium carbonate that has favorable attachment sites for bacteria. The detachment rate was less than 0.02 of the attachment rate, indicating irreversible attachment of bacteria. High reduction rate of bacteria was also attributed to soil calcium carbonate.
Bacteria isolated from the gut of different developmental stages of Phlebotomus duboscqi Neveu-Lemaire, 1906 belonged almost all to aerobic or facultatively anaerobic gram-negative rods. In females, the highest bacterial counts were observed two days after bloodfeeding; seven days after bloodfeeding the bacterial counts returned to pre-feeding levels. Most isolates were identified phenotypically as Ochrobactrum sp. The distinctiveness and homogeneity of the phenotypic and genotypic characteristics of Ochrobactrum isolates indicated that they belonged to a single strain (designated AK). This strain was acquired by larvae from food and passaged transtadially: it was isolated from the guts of fourth-instar larvae shortly before pupation, from pupae as well from newly emerged females. Most other bacteria found in females were acquired from the sugar solution fed to adults. To determine if the midgut lectin activity may serve as antibacterial agent females were membrane-fed on blood with addition of inhibitory carbohydrates. No significant differences in bacterial infections were found between experimental and control groups and we suppose that the lectin activity has no effect on gram-negative bacteria present in sandfly gut.
Knihovna Národního muzea Praha CZ 53 C 34, Královská kanonie premonstrátů na Strahově - Strahovská knihovna Praha CZ BX VI 30/19, Královská kanonie premonstrátů na Strahově - Strahovská knihovna Praha CZ BU II 132/37 def., Královská kanonie premonstrátů na Strahově - Strahovská knihovna Praha CZ BU II 133/21, Národní knihovna ČR Praha CZ 65 E 3387 def., Národní knihovna ČR Praha CZ 46 C 181 adl. 12, Klášter dominikánů - knihovna Praha CZ E III 137, Klášter Rytířského řádu křižovníků s červenou hvězdou - knihovna Praha CZ XV G 4 adl. 8, Knihovna Akademie věd ČR Praha CZ TF 347 adl. 23 def., Vědecká knihovna v Olomouci Olomouc CZ 602.419, Vědecká knihovna v Olomouci Olomouc CZ 602.271 adl. 4, and BCBT07614
We studied the diet of the badger through scat analysis and used seven previously described methods to assess their comparability. Methods compared included those based on frequencies of occurrence of different food items and volumetric methods. Our results showed that, depending on the basic methodological procedure, we could classify methods in two groups: frequencies of appearance and volumetric methods. The depiction of the diet obtained is quite similar within these groups but differs between them, as each group depicts different aspects of the trophic ecology of the badger. In conclusion, we advise the use of more than one method when assessing the diets of badgers or other carnivores. The best option is the use of a frequency-based method combined with one or two volumetric methods.
Bagrichthys majusculus, a new species of bagrid catfish from Indochina, is very similar to B. macracanthus and B. vaillantii, and has been previously identified as the former species. It differs from congeners in having a unique combination of the following characters: relatively large and broad mouth, well-developed oral dentition with homodont teeth, 10–13 gill rakers, moderately-long dorsal spine with 15–27 serrations, 9 pectoral-fin rays, inner and outer mandibular barbels with straight margins, pectoral-spine length 15.8–20.7 % SL (standard length), dorsal-spine length 24.4–32.5 % SL, length of adipose-fin base 46.0–50.7 % SL, adipose maximum height 9.9–10.5 % SL, depth of caudal peduncle 7.1–7.5 % SL, and head depth 14.0–16.1 % SL.