The karyotype, C-banding, and nucleolar organizer regions (NORs) of six specimens of Hemiechinus auritus from Turkey were examined. The diploid number of chromosomes was 2n = 48, the fundamental number of chromosome arms FN = 96, and the number of autosomal arms FNa = 92. Most of the chromosomes possessed centromeric constitutive heterochromatin, except of the pairs nos. 4, 6, 7, 8, 9, 12, and 17. The X and Y chromosomes appeared to be euchromatic, and possible geographic variation in their morphology was indicated in comparison with previously published data. The NORs were located in the terminal regions of the long arms of four metacentric or submetacentric chromosomes. The localization of the NORs was not associated with C-positive autosomal regions as in hedgehogs of the genus Erinaceus.
Non-small cell lung cancer (NSCLC) results in high mortality and has gained increasing attention. C-Phycocyanin (C-PC) has been identified as a potential therapeutic inhibitor for NSCLC, but its underlying mechanism remains obscure. The gene expression of the long noncoding RNA neighbour of BRCAI RNA 2 (NBR2) in NSCLC cells was evaluated by quantitative reverse transcription-PCR. The cell capacity for proliferation and migration was examined by EdU and wound-healing assays. Furthermore, the viability and apoptosis of cells was measured with CCK-8 and annexin V/PI, respectively. Next, the protein level of activation of adenosine monophosphate- activated protein kinase and the rapamycin kinase (mTOR) signalling pathway-associated molecules was evaluated by western blotting. H292 cells were pre-treated with C-PC or transfected with plasmids encoding NBR2 or the shNBR2 plasmid, to over-express or knock down NBR2 expression, respectively. NBR2 expression was robustly down-regulated in NSCLC cell lines compared with a normal cell line (BEAS-2B). NBR2 over-expression inhibited migration and promoted apoptosis of H292 cells. Treatment of H292 cells with C-PC enhanced NBR2 levels in a dose- and time-dependent manner. Downregulation of NBR2 in H292 cells inhibited the activity of C-PC on cell proliferation, viability and clone formation. Further mechanistic investigation showed that the down-regulation of NBR2 abolished the modulatory effects of C-PC on the AMPK/mTOR signalling pathway. In conclusion, C-PC inhibits H292 cell growth by enhancing the NBR2/AMPK signalling pathway.
Of the total 570 species, 194 species in 116 genera and 52 families were found with C3 photosynthesis, 24 species in 17 genera and 6 families with C4 photosynthesis, and 2 species in 1 genera and 1 family with CAM photosynthesis. 90 % of the total species can be found in Changbai Mountain flora, more a half (69 %) in North China flora, and about 1/3 in Mongolian flora and Xinan flora, respectively. The occurrence of C4 species was not as common as that in adjacent grasslands and deserts, but relatively more than in the adjacent forests. Of the total 24 C4 species, 63 % C4 species (15 of 24) was found in Gramineae. Nine life form types can be found, reflecting the moist climate in the region, especially the occurrence of epiphyte and liana forms. Relatively more geophyte life form plants suggested the winter in the region was much colder than in grasslands. These indicated that both ecological studies and land management decisions must take into account plant photosynthetic pathway and life form patterns, for both of them are closely related to climatic changes and land use.
C4 photosynthetic pathway and morphological functional types were determined for 104 species in 45 genera and 10 families from the deserts of China. 67 C4 species (64.4 %) were found in Dicotyledoneae (e.g. Chenopodiaceae, Polygonaceae, and Amaranthaceae), the other 37 species were in Monocotyledoneae (e.g. Gramineae, Cyperaceae, and Commelinaceae). 36.5 % of the Chenopodiaceae species (predominantly members of the genera Anabasis, Atriplex, Kochia, Salsola, and Suaeda) identified in the desert regions were found with C4 photosynthesis, which was about 48 % of the total C4 species. Many C4 species (58.7 %) were annuals (e.g. Amaranthus, Atriplex, Digitaria, Eragrostis, Kochia, and Salsola) and experienced long-term droughts, high temperature, and high irradiance. Relatively more shrub C4 species (28 species of 104) were found in Chenopodiaceae (e.g. Anabasis, Camphorosma, Haloxylon, and Salsola) and Polygonaceae (e.g. Calligonum) in the desert regions. Most of shrub C4 species with small leaf area were no more than 1 m in height and distributed in sandy soils. Composition of relatively more annual species, shrubs, and Chenopodiaceae C4 species was the primary characteristic for the C4 species occurrence in deserts, and this was remarkably related with the arid environmental conditions.
Floristic composition, life forms for C4 species, and the pattern of altitude distribution were studied on Tibetan Plateau. 79 species, in 7 families and 46 genera, were identified with C4 photosynthesis. 95 % of these C4 species belong to Gramineae (51 species), Cyperaceae (14 species), and Chenopodiaceae (10 species), indicating that C4 plants mainly occur in very few families (7 of 204) on the Tibetan Plateau. High altitude distribution for all the Chenopodiaceae C4 species (> 3 000 m above sea level) suggests that plants of this kind have large tolerance to cold, dryness, and strong ultraviolet radiation. Most Gramineae and Cyperaceae C4 species occurrences are consistent with extensive distribution of steppes and meadows in the vast flat of the central Plateau (1 000-3 000 m a.s.l.). Relatively high amount of hemicryptophyte form plants (44 %) in the region indicates that the vegetation, especially grassland, meadows, and steppe, are in good condition. There is a strong relationship between numbers of C4 species and altitude in the Tibetan Plateau. Occurrence of C4 species is significantly less in both high and low altitude plateaux in Tibet. Altitude distribution pattern for C4 species in the region is not only consistent with the altitude and climate, but also with the vegetation types in altitude gradient.