The resting membrane potential (Vm) of isolated somatic longitudinal muscles of the earthworm Lumbricus terrestris was studied by glass microelectrodes. The inhibition of chloride permeability by low pH did not affect Vm of the muscle fibers in isolated somatic longitudinal muscles of the earthworm Lumbricus terrestris which was -48.7 mV (inside negative) at pH 7.3 and -49.1 at pH 5.6. On the other hand, bathing the muscles in Cl- and Na+-free solutions, or application of the chloride transporter inhibitor furosemide and Na+-K+-ATPase inhibitor ouabain depolarized the Vm by 3-5 mV. The effects of a Cl- -free solution and ouabain were not additive. This demonstrates relatively small contribution of equilibrium potential for Cl- to the resting membrane potential and electrogenic effect of Na+K+-ATPase which is dependent on the supply of Na+i ions by furosemide-sensitive and Cl-e- and Na+e-dependent electroneutral transport (most probably Na+K+Cl- cotransport)., E. M. Volkov, L. F. Nurullin, E. Nikolsky, J. Krůšek, F. Vyskočil., and Obsahuje bibliografii
Simultaneous measurements of chlorophyll (Chl) fluorescence and photosynthetic O2 evolution {P^ in leaf disks from Vicia faba (cv. Reina Blanca) leaves were doně during the first weeks of growth in order to evaluate the damage caused by methabenzthiazuron (MBT) under field conditions. The plants were treated at preemergence with two doses of MBT (0.25 .and 0,40 g m'^). Measurements were carried out at constant temperature and saturating CO2 concentration. During the first weeks after plant emergence both doses of herbicide were followed by a drop in and in some fluorescence parameters, such as Fy/Fn, and AF/Fn,' ratios, and in photochemical and non-photochemical quenching, qp and q^. At the same time, increases in minimal fluorescence (Fq and Fq') and in maximal (Fj„') and steady-state fluorescence (Fj) were aiso observed on application of saturating pulses. Fluorescence parameters gave a better indication of the damage caused to the photosynthetic systém than the measurements. The linear relationship found between OO2 and the effective quantum yield, AF/Fn,', suggests that the latter is a good measure of the quantum efficiency of photosynthesis in herbicide-treated plants. Moreover, plant tissues detoxified the herbicide and, one month after plant emergence, photosynthesis was fully recovered in herbicide-treated plants; biomass production recovered and even increased at the end of the growth period.
The effect of inorganic (IC) depletion on fluorescence quenching was studied under laboratory conditions using the chlorococcal alga Scenedesmus quadricauda strain Greifswald/15. The absence of IC caused a decrease in photochemical quenching (^p), fluorescence yield and the photosystem 2 photochemical yield (dF/F^), and an increase of non-photochemical quenching {q^). High extemal pH (about 11), which accompanies the IC-depletion, did not háve any remarkable effect on the algae. Fluorescence parameters were reversed by a resupply of CO2. The IC-defícient algae when exposed to high irradiance appeared to be less sensitive to the photoinhibition than the algae supplied with CO2. Increased thermal dissipation of the excitation energy (non-photochemical quenching) in the absence of IC is one of the probable protective mechanisms against photoinhibitory damage.