The reports of analgesic effects of benzodiazepines are inconsistent. There is evidence of a hyperalgesic effect induced by activation of supraspinal GABAA receptors and an antinociceptive effect induced by activation of receptors located in the spinal cord (dorsal horns). The aim of the study was to discover whether the systemic administration of a benzodiazepine agent alprazolam increases the systemic analgesic efficacy of non-opioid analgesic ibuprofen. Experimental studies combining these agents have not yet been published. We used three experimental methods - writhing test (with acetic acid), tail-flick test and plantar test to assess analgesic action. The drugs were administered orally. Augmentation of the analgesic effect of ibuprofen by alprazolam was proved for the writhing test at a dose of 30 mg/kg of ibuprofen and alprazolam 1 mg/kg. The reaction time of the combination was significantly prolonged in comparison with ibuprofen alone. The results of the tail-flick test and plantar test were negative. The effect of ibuprofen was not enhanced by alprazolam in tests of acute thermal pain. Our results have demonstrated that the analgesic action of ibuprofen is only weakly enhanced by alprazolam., T. Doležal, M. Kršiak., and Obsahuje bibliografii
a1_Predchádzajúce výskumy naznačujú, že autenticita (pravdivosť v prežívaní a správaní) súvisí s lepším fungovaním blízkych vzťahov. Štúdia je zameraná najmä na partnerskú, resp. vzťahovú autenticitu, teda tendenciu byť autentický voči partnerovi vo vzťahu. Výskum bol zameraný na zistenie korelačných súvislostí autenticity so vzťahovou autonómiou, so sebaúctou, so spokojnosťou a mierou investícií do vzťahu, s dimenziami lásky a s mierou vnímanej prepojenosti partnerov, a to s cieľom overiť, či autenticita partnerov môže predikovať kvalitu partnerského vzťahu. V rámci partnerských dvojíc bola zisťovaná aj miera zhody medzi autenticitou žien a autenticitou mužov, a tiež to, či autenticita jedného partnera súvisí s aspektmi vzťahu sledovanými u druhého partnera (tzv. efekt partnera). Údaje boli zozbierané prostredníctvom batérie dotazníkov (Škála vzťahovej autenticity, Rolový dotazník autenticity, Dotazník autonómie v partnerskom vzťahu, Škála trojuholníku lásky, dimenzie spokojnosť a investície zo Škály investičného modelu, Rosenbergova škála sebahodnotenia a Škála zahrnutosti druhého v sebe) od 189 respondentov vo veku od 18 do 33 rokov, z toho 51 partnerských párov. Výsledky poukázali na štatisticky významné korelácie medzi partnerskou autenticitou a všetkými sledovanými aspektmi partnerského vzťahu, ako aj na nenáhodnú podobnosť partnerov v miere autenticity. Tiež sa preukázal pozitívny efekt autenticity partnera na vzťahovú autonómiu a spokojnosť druhého partnera (u oboch pohlaví). Čo sa týka dimenzií lásky, u žien bol zistený pozitívny efekt autenticity na oddanosť mužov, kým u mužov pozitívny efekt autenticity na mieru intimity žien., a2_ Určitým limitom výskumu môže byť jeho sebavýpoveďový charakter, sociálna žiaducosť sledovaných charakteristík a špecifickosť výskumnej vzorky (prevažne vysokoškolskí študenti, relatívne vysoký podiel študentov psychológie)., b1_Previous research suggests that authenticity (the truth in the experiences and behaviors) is associated with better functioning of close relationships. The study is mainly focused on the partner or relational authenticity, i.e. the tendency to be authentic towards the partner in the relationship. The research was aimed at finding out the correlations of authenticity with relational autonomy, self-esteem, satisfaction and the level of investment in the partnership, dimensions of love, and the extent of perceived connectedness between partners, to verify whether the authenticity of partners may predict the quality of partnership. Then the level of agreement between the authenticity of women and authenticity of men within the couples was assessed, as well as whether the authenticity of one partner is related to aspects of relationship of the other partner (so-called partner´s effect). Data were collected through the battery of questionnaires (Partnership authenticity scale, Role authenticity questionnaire, Autonomy in partnership questionnaire, Triangular love scale, satisfaction and investment dimensions of the Investment model scale, Rosenberg self-esteem scale, and Inclusion of other to the self scale) from 189 respondents aged 18-33 years, including 51 couples. The results showed statistically significant correlations between relational authenticity and all monitored aspects of the partnership, as well as nonrandom similarity between partners in the level of their authenticity. It was also showed the positive effect of partner’s authenticity to relational autonomy and satisfaction (in both sexes). Regarding the dimensions of love, it was found the positive effect of women’s authenticity to the men’s commitment, and positive effect of men’s authenticity to level of women’s intimacy., b2_Certain limits of the research can be its self-report character, social desirability of observed characteristics, and specificity of the research sample (mostly university students, with relatively high proportion of psychology students)., Eva Nábělková, Natália Balážová., and Obsahuje seznam literatury
We investigated the gastric response to an ulcerogenic irritant and the change in gastric functions in an experimental rat model of obstructive jaundice, with or without biliary drainage. After biliary obstruction for 14 days, rats with ligated bile duct (BDL) were randomly divided into three groups: BDL group without biliary drainage, BDL followed by choledochoduodenostomy (CD) or a choledochovesical fistula (CVF). The gastric functions were evaluated 2 weeks after the surgery. Gastric damage, induced by orogastric administration of ethanol, was evaluated 30 min later using a lesion index and microscopic scoring was then performed on fixed stomachs. Basal gastric acid secretion was measured by the pyloric ligation method.The lesion index and maximum lesion depth did not differ in the BDL and sham groups, while they were significantly reduced in the CD group. Gastric acid output and secretory volume were reduced in the BDL group compared to the sham group, while these reductions were abolished in the CD group. Afferent denervation with capsaicin further reduced the ulcer index in the later group. Our data suggest that gastric mucosal susceptibility to injury is dependent on the normal flow of bile into the duodenal lumen, which appears to be a requirement for adaptive gastric cytoprotection., A. Cingi, R. Ahiskali, B. K. Oktar, M. A. Gülpinar, C. Yegen, B.Ç. Yegen., and Obsahuje bibliografii
Studie Evy Veselovské se zabývá notovanými kodexy, které vznikly na území dnešního Slovenska od 14. do začátku 16. století a do jejichž způsobu notace se promítl vliv českého kulturního prostředí., Recent research of Slovakian medieval notated codices and manuscript fragments raised an important fact: the written culture of the late 14th and 15th centuries in Slovakia was strongly influenced by education from Czech lands. Particularly between 1370 and 1520, the direct impact of the scribal notation tradition from Czech lands to Slovak area can be detected in a number of Slovakian music sources. Codices and dozens of manuscript fragments documenting Bohemian notation in the Slovak geographical area have become the subject of research, along with the systematization, analysis and evaluation of all currently known and edited medieval notated sources from Slovakia. The main purpose of this research is to organise the information gained from these sources, and to determine the general structural features of Bohemian notation in Slovakia., Eva Veselovská., Rubrika: Studie, and Slovenské resumé na s. 376, anglický abstrakt na s. 337.
Angiotensin converting enzyme (ACE) inhibition has been reported to induce regression of hypertrophy in several models of hemodynamic pressure overload. The aim of the present study was to determine whether the ACE inhibitor captopril can reduce hypertrophy of the left ventricle induced by a chronic volume overload and modify collagen composition of the hypertrophied myocardium. Rabbits with four months lasting aortic insufficiency were divided into two groups: treated with captopril (20 mg/kg/day) for five weeks and treated with placebo. The respective control groups were represented by sham-operated animals. Aortic insufficiency induced a decrease of diastolic pressure, an increase of systolic and pulse pressure, hypertrophy of the left and right ventricle, and an increase of hydroxyproline content in the left ventricle without a change of hydroxyproline concentrations in either ventricle. Captopril treatment further enhanced pulse pressure by decreasing diastolic blood pressure. Hypertrophy of the left ventricle, hydroxyproline content and concentration in both ventricles were unaffected by captopril treatment. It is concluded that ACE inhibition did not reverse the left ventricular hypertrophy developed as a result of overload induced by aortic insufficiency. We suggest that mechanisms different from activation of the renin-angiotensin system may play a decisive role in the maintenance of hypertrophy in this particular model of volume hemodynamic overload., F. Šimko, V. Pelouch, J. Kyselovic., and Obsahuje bibliografii
Pyridoxal isonicotinoyl hydrazone (PIH) is a new tridentate Fe-chelating agent that should be very promising in many pathological states resulting from both an iron-overload and formation of free radicals. The aim of our study was to investigate the effect of PIH on the cardiovascular system focusing to the regulatory protein - cardiac troponin T (cTnT). The study was carried out in two groups of Chinchilla male rabbits: 1) PIH (50 mg/kg dissolved in 10 % Cremophor i.p., once a week, 10 administrations, n=8) and 2) Cremophor (2 ml/kg i.p. in the same schedule, n=7). Plasma concentrations of cTnT (as a marker of myocardial damage) were measured using a commercial kit (Roche). cTnT was within the physiological range (i.e. < 0.1 mg/l) during the whole experiment in the Cremophor group. In the PIH group, the cTnT levels were not significantly increased when compared with the control group or with the initial values (except with those before the 5th administration). Furthermore, we analyzed the cytosolic and myofibrillar fraction of cTnT in the left ventricular myocardium. Using SDS-PAGE and Western blot we resolved three isoforms. The profiling of TnT did not differ significantly between the PIH-treated group and the Cremophor-treated group. Our data concerning cTnT support the opinion that the possible cardiotoxicity of PIH is very low., M. Adamcová, J. Macháčková, V. Geršl, V. Pelouch, T. Šimůnek, I. Klimtová, R. Hrdina, P. Poňka., and Obsahuje bibliografii
a1_The question was addressed of how nitric oxide synthase (NO synthase) inhibition-induced hypertension in rat parents would affect the cardiovascular system in their offsprings. Two experimental groups were set up: Group I - offsprings of parents who had both been administered NO synthase inhibitor L-nitro-arginine methyl ester (L-NAME 40 mg/kg/day) for 5 weeks, the treatment of dams continued till week 12. Group II - offsprings fed by dams administered L-NAME after delivery only for a period of 4 weeks. Control age-matched offsprings formed the third group. Blood pressure and heart rate in parents and in 3-week-old offsprings were determined noninvasively. In the offsprings, body and heart weight were measured and the heart/body weight ratio (HW/BW) was calculated. The NO synthase activity, and also ornithine decarboxylase activity as a marker of polyamine production, were determined in the heart. The acetylcholine-induced relaxation of aortic rings was also followed. A marked blood pressure increase with a tendency to a decreased heart rate was found in the offsprings of Group I. A significant decrease in heart weight and body weight with a decreased HW/BW ratio indicated cardiac hypotrophy that contrasted with the decrease in NO synthase activity and increase in ornithine decarboxylase activity in the heart. Noteworthy was also the finding of completely preserved relaxation of the aorta to acetylcholine. Offsprings of Group II were similarly characterized by significantly higher blood pressure, a tendency to decreased heart rate, a decrease in heart weight, but not of the HW/BW ratio. The contrasting findings of heart weight decrease on the one hand and NO synthase activity decrease and ornithine decarboxylase increase on the other, were also found in this group. Full relaxation of the aorta to acetylcholine was preserved., a2_It can be concluded that remarkable alterations in the cardiovascular system were found in offsprings of hypertensive NO compromised parents., M. Gerová, I. Bernátová, J. Török, M. Juráni., and Obsahuje bibliografii
Changes in extracellular space (ECS) diffusion parameters, DC potentials and extracellular potassium concentration were studied during single and repeated cortical spreading depressions (SD) in 13-15 (P13-15), 21 (P21) and 90-day-old (adult) Wistar rats. The real-time iontophoretic method using tetramethylammonium (TMA+)-selective microelectrodes was employed to measure three ECS parameters in the somatosensory cortex: the ECS volume fraction α (α = ECS volume/total tissue volume), ECS tortuosity λ (increase in diffusion path length) and the nonspecific TMA+ uptake k’. SD was elicited by needle prick. SD was significantly longer at P13-15 than at P21 and in adults. During SD, α in all age groups decreased from 0.21-0.23 to 0.05-0.09; λ increased from 1.55-1.65 to 1.95-2.07. Ten minutes after SD, α (in adults) and λ (all age groups) increased compared to controls. This increase persisted even 1 hour after SD. When SD was repeated at 1 hour intervals, both α and λ showed a gradual cumulative increase with SD repetition. Our study also shows that cortical SD is, as early as P13, accompanied by severe ECS shrinkage and increased diffusion path length (tortuosity) with values similar to adults, followed by a long-lasting increase in ECS volume and tortuosity when compared to pre-SD values., T. Mazel, F. Richter, L. Vargová, E. Syková., and Obsahuje bibliografii
The role of the cortico-tectal pathways in the processing of auditory signals was investigated by recording the click-evoked responses and extracellular multiple unit activity in the inferior colliculus (IC) after functional ablation of the auditory cortex (AC) by local intracortical application of a sodium channel blocker, tetrodotoxin (TTX). Click-evoked IC responses (IC-ER) and multiple unit activity in response to tone bursts were recorded with implanted electrodes in the IC of rats lightly anaesthetized with xylazine. Neural activity was recorded before and after the application of TTX into the ipsilateral auditory cortex (AC) through three implanted cannulas in a total dose of 30 ng. The functional status of the AC was monitored by recording click-evoked middle latency responses from a ball electrode implanted on the AC. During inactivation of the AC, IC-ER amplitudes were either increased (48 % of the cases), decreased (32 % of the cases) or not evidently changed (20 % of the cases). Corresponding effects were observed in the firing rate of IC neurons. Functional ablation of the AC also resulted in a significant prolongation of the latencies of individual waves of the IC-ER. However, the discharge pattern of the multiple unit responses, response thresholds and tuning were not altered during AC inactivation. IC neural activity recovered within several hours, and maximally during 2 days. The results reveal principles of the interaction of cortico-tectal pathways with IC neuronal activity., F. C. Nwabueze-Ogbo, J. Popelář, J. Syka., and Obsahuje bibliografii
The aim of this study was to assess the molecular basis of renal Na,K-ATPase disturbances in response to NO-deficient hypertension induced in rats by NO-synthase inhibition with 40 mg/kg/day NG-nitro-L-arginine methyl ester (L-NAME) for four weeks. After 4-week administration of L-NAME, the systolic blood pressure (SBP) increased by 30 %. Three weeks after terminating the treatment, SBP recovered to control value. When activating the Na,K-ATPase with its substrate ATP, a 36 % increase in Km and 29 % decrease in Vmax values were observed in NO-deficient rats. During activation with Na+, the Vmax was decreased by 20 % and the KNa was increased by 111 %, indicating a profound decrease in the affinity of the Na+-binding site in NO-deficient rats. After spontaneous recovery from hypertension, the Vmax remained at the level as in hypertension for both types of enzyme activation. However, in the presence of lower concentrations of substrate which are of physiological relevance an improvement of the enzyme activity was observed as documented by return of Km for ATP to control value. The KNa value for Na+ was decreased by 27 % as compared to hypertension, but still exceeded the corresponding value in the control group by 55 % thus resulting in a partial restoration of Na+ affinity of Na,K-ATPase which was depressed as a consequence of NO-dependent hypertension., N. Vrbjar, V. Javorková, O. Pecháňová., and Obsahuje bibliografii