EEG activities with open eyes in a quiet state (OA), during the pseudo-Raven's test (PRA), in hypnagogic state (HYP) and in the course of REM sleep (REM) are characteristic by nearly flat curves. We observed the states with eyes closed (OC), with hyperventilation (HV), during mental activity of calculation (CAL) and in NONREM 1 sleep (NR 1). 24 tested persons (probands) were investigated. We have found 8 typical states of EEG signals, which all have relation to attention and mental activity. Consequently, the EEG analysis can help in the differentiation between the above eight states. Using similar analyses, it is possible to discriminate all stages of NONREM and REM sleep without polysomnography.
Decrease of attention and an possible micro-sleep of an artificial system operator is very dangerous and its early detection can prevent great losses. This article deals with a classification of States of vigilance based on the analysis of an electroencefalographic activity of the brain. Preprocessing of data is done by the Discrete Fourier Transform. For the recognition radial basis functions (RBF), a k-nearest neighbor and a method based on the Bayesian theory is used. Its coefficients are found using the maximum likelihood estimation. An experiment with recognition of 6 States of vigilance created according to reaction time is performed.
The entropy region is a fundamental object of study in mathematics, statistics, and information theory. On the one hand, it involves pure group theory, governing inequalities satisfied by subgroup indices, whereas on the other hand, computing network coding capacities amounts to a convex optimization over this region. In the case of four random variables, the points in the region that satisfy the Ingleton inequality (corresponding to abelian groups and to linear network codes) form a well-understood polyhedron, and so attention has turned to Ingleton-violating points in the region. How far these points extend is measured by their Ingleton score, where points with positive score are Ingleton-violating. The Four-Atom Conjecture stated that the Ingleton score cannot exceed 0.089373, but this was disproved by Matúš and Csirmaz. In this paper we employ two methods to investigate Ingleton-violating points and thereby produce the currently largest known Ingleton scores. First, we obtain many Ingleton-violating examples from non-abelian groups. Factorizability appears in many of those and is used to propose a systematic way to produce more. Second, we rephrase the problem of maximizing Ingleton score as an optimization question and introduce a new Ingleton score function, which is a limit of Ingleton scores with maximum unchanged. We use group theory to exploit symmetry in these new Ingleton score functions and the relations between them. Our approach yields some large Ingleton scores and, using this methodology, we find that there are entropic points with score 0.09250007770, currently the largest known score., Nigel Boston and Ting-Ting Nan., and Obsahuje bibliografické odkazy
Development of combustion engines can be characterized by continuous increase in the number of computational simulations being applied in all areas. There is a clear trend to use chain or belt drives for the design of timing drives. Computational simulation of these drives has not been developing too long due to high demands on the computational technology. The paper focuses on simulation of dynamics of the timing chain drive with the use of a multi-body system. A mass-produced four cylinder in-line engine with two camshafts and two valves per cylinder has been used as a computational model. and Obsahuje seznam literatury
The Leishmania metalloproteinase GP63 has been reported to play important roles mainly in resistance of promastigotes to complement-mediated lysis and in interaction with macrophage receptors. On the other hand, its function in insect vectors is still unclear. We compared the structure and dosage of gp63 genes and the activity of GP63 in Leishmania major Yakimoff et Schokhor strains and lines differing in virulence for mice and ability to develop in sand flies. The results demonstrate considerable variability in amount and proteolytical activity of GP63 among L. major strains although genomic changes in the gp63 locus were not found. Attenuated LV561/AV line showed low amount and low enzymatic activity of GP63. Serial passages of attenuated parasites through either Phlebotomus duboscqi Neveu-Lemaire or through mice led to a recovery of GP63 proteolytical activity to the level present in virulent LV561/V line. Overexpression of GP63 was found in two L. major strains (L119, Neal) with defective lipophosphoglycan (LPG); both these strains were capable to cause mice infection but unable to survive and multiply in sand flies. Differences were found also in karyotypes and in amount of minichromosomes amplified in some lines of the LV561 strain. The results suggest that parasite virulence is not simply correlated with the activity of GP63; however, this enzyme plays a significant role in association with other surface molecules, especially LPG. Overexpression of GP63 can compensate LPG defect in the vertebrate host but in sand flies both molecules fulfil quite different functions and the defect in LPG is lethal for the parasite. On the other hand, linear minichromosomes of about 200 kb found in some lines of the LV561 strain are associated with development in vitro and in the vector but they are not essential for the infection of the vertebrate host.
Mechanical behavior of biological structures under dynamic loading generally depends on elastic as well as viscous properties of biological materials. The significance of “viscous” parameters in real situations remains to be elucidated. Behavior of rheological models consisting of a combination of inertial body and two Voigt’s bodies were described mathematically with respect to inverse problem solution, and behavior in impulse and harmonic loadings was analyzed. Samples of walls of porcine and human aorta thoracica in transverse direction and samples of human bone (caput femoris, substantia compacta) were measured. Deformation responses of human skin in vivo were also measured. Values of elastic moduli of porcine aorta walls were in the interval from 102 kPa to 103 kPa, values of viscous coefficients were in the interval from 102 Pa.s to 103 Pa.s. The value of shear stress moduli of human caput femoris, substantia compacta range from 52.7 to 161.1 MPa, and viscous coefficients were in the interval from 27.3 to 98.9 kPa.s. The role of viscous coefficients is significant for relatively high loading frequencies - in our materials above 8 Hz in aorta walls and 5 Hz for bones. In bones, the viscosity reduced maximum deformation corresponding to short rectangular stress., M. Kuchařová, S. Ďoubal, P. Klemera, P. Rejchrt, M. Navrátil., and Obsahuje bibliografii
Visfatin is a multi-functional molecule that can act intracellularly and extracellularly as an adipokine, cytokine and enzyme. One of the main questions concerning visfatin is the mechanism of its secretion; whether, how and from which cells visfatin is released. The objective of this in vitro study was to observe the active secretion of visfatin from 3T3-L1 preadipocytes and adipocytes, HepG2 hepatocytes, U-937, THP-1 and HL-60 monocytes and macrophages. The amount of visfatin in media and cell lysate was always related to the intracellular enzyme, glyceraldehyde-3- phosphate dehydrogenase (GAPDH), to exclude the passive release of visfatin. Visfatin was not found in media of 3T3-L1 preadipocytes. In media of 3T3-L1 adipocytes and HepG2 hepatocytes, the ratio of visfatin to the amount of GAPDH was identical to cell lysates. Hence, it is likely that these cells do not actively secrete visfatin in a significant manner. However, we found that significant producers of visfatin are differentiated macrophages and that the amount of secreted visfatin depends on used cell line and it is affected by the mode of differentiation. Results show that 3T3-L1 adipocytes and HepG2 hepatocytes released visfatin only passively during the cell death. U-937 macrophages secrete visfatin in the greatest level from all of the tested cell lines., P. Svoboda, E. Křížová, K. Čeňková, K. Vápenková, J. Zídková, V. Zídek, V. Škop., and Obsahuje bibliografii
Never before in history data has been generated at such high volumes as it is today. It is estimated that every year about 1 Exabyte (= 1 Million Terabyte) of data are generated, of which a large portion is available in digital form. Exploring and analyzing the vast volumes of data becomes increasingly difficult. This paper describes system Vitamin-S that aims to help when analyzing very large data sets.
Visual cognitive responses (P300) to moving stimuli were tested in 36 subjects with the aim to find the normal range of P300 parameters. Concomitantly, the circadian intra-individual variability of the P300 was studied in a subgroup of 6 subjects. Visual stimuli consisted of either coherent (frequent stimulus) or non-coherent motion (random stimulus). The oddball paradigm was applied for recording cognitive responses. P300 to rare stimuli had an average latency of 447.3±46.6 ms and amplitude of 12.9±6.0 mV. The average reaction time was in the range from 322 to 611 ms and there was no correlation between the reaction time and P300 latency. We did not find any significant circadian changes of the P300 parameters in the 6 subjects tested four times during the same day. Cognitive (event-related) responses (P300) displayed distinctly greater inter-individual variability (S.D. of 50 ms) when compared with pattern-reversal and motion-onset VEPs (S.D. of 6.0 ms and 14 ms, respectively). For this reason, the clinical use of P300 elicited by this kind of visual stimuli seems to be rather restricted and the evaluation of its intra-individual changes is preferable., Z. Kubová, J. Kremláček, J. Szanyi, J. Chlubnová, M. Kuba., and Obsahuje bibliografii