Tématem článku je experimentální výzkum spektrálních vlastností turbulence ve slunečním větru v oblasti přechodu mezi magnetohydrodynamickým (MHD) a kinetickým režimem magnetizovaného plazmatu. Výzkum je založen na měření parametrů plazmatu s vysokým časovým rozlišením, kterého bylo dosaženo přístrojem BMSW (Bright Monitor of Solar Wind) navrženým a vyvinutým na Katedře fyziky povrchů a plazmatu MFF UK v posledních letech. Získané výsledky jsou aplikovatelné nejen na variace meziplanetárního nebo mezihvězdného plazmatu, ale i na plazma ve fúzních systémech., This paper deals with experimental investigations of the spectral properties of solar wind turbulence during transition from magnetohydrodynamic (MHD) to kinetic regimes of a magnetized plasma. The study is based on the measurements of solar wind parameters with very high time resolution, which was achieved using the BMSW instrument designed and developed at the Department of Surface and Plasma Sciences of Faculty of Mathematics and Physics, Charles University in Prague. The results of these investigations can be applied not only for astrophysical plasmas but also for plasmas in fusion systems., Jana Šafránková, Zdeněk Němeček, František Němec, Lubomír Přech., and Obsahuje bibliografii
Předkládáme stručnou informaci o využití polarizačních vlastností světla ve spektroskopické elipsometrii. Ukazujeme příklady aparatur a diskutujeme ilustrativní spektra. Vybíráme několik ukázek aplikací a současných trendů., We provide brief information on the use of light polarisation in spectroscopic ellipsometry. Examples of experimental setups are presented and illustrative spectra are discussed. We also select a few topics in applications and current trends., Josef Humlíček., and Obsahuje seznam literatury
Targeting polyamines of parasitic protozoa in chemotherapy has attracted attention because polyamines might reveal novel drug targets for antiparasite therapies (Müller et al. 2001). The biological function of the triamine spermidine in parasitic protozoa has not been studied in great detail although the results obtained mainly imply three different functions, i.e., cell proliferation, cell differentiation, and biosynthesis of macromolecules. Sequence information from the malaria genome project databases and inhibitor studies provide evidence that the current status of spermidine research has to be extended since enzymes of spermidine metabolism are present in the parasite (Kaiser et al. 2001). Isolation and characterisation of these enzymes, i.e., deoxyhypusine synthase (EC 1.1.1.249) (DHS) and homospermidine synthase (EC 2.5.1.44) (HSS) might lead to valuable new targets in drug therapy. Currently research on spermidine metabolism is based on the deposition of the deoxyhypusine synthase nucleic acid sequence in GenBank while the activity of homospermidine synthase was deduced from inhibitor studies. Spermidine biosynthesis is catalyzed by spermidine synthase (EC 2.5.1.16) which transfers an aminopropyl moiety from decarboxylated S-adenosylmethionine to putrescine. Spermidine is also an important precursor in the biosynthesis of the unusual amino acid hypusine (Wolff et al. 1995) and the uncommon triamine homospermidine in eukaryotes, in particular in pyrrolizidine alkaloid-producing plants (Ober and Hartmann 2000). Hypusine is formed by a two-step enzymatic mechanism starting with the transfer of an aminobutyl moiety from spermidine to the ε-amino group of one of the lysine residues in the precursor protein of eukaryotic initiation factor eIF5A by DHS (Lee and Park 2000). The second step of hypusinylation is completed by deoxyhypusine hydroxylase (EC 1.14.9929) (Abbruzzese et al. 1985). Homospermidine formation in eukaryotes parallels deoxyhypusine formation in the way that in an NAD+-dependent reaction an aminobutyl moiety is transferred from spermidine. In the case of homospermidine synthase, however the acceptor is putrescine. Thus the triamine homospermidine consists of two symmetric aminobutyl moieties while there is one aminobutyl and one aminopropyl moiety present in spermidine. Here, we review the metabolism of the triamine spermidine with particular focus on the biosynthesis of hypusine and homospermidine in parasitic protozoa, i.e., Plasmodium, Trypanosoma and Leishmania, compared to that in prokaryotes i.e., Escherichia coli, a phytopathogenic virus and pyrrolizidine alkaloid-producing plants (Asteraceae) and fungi.
Spermatological characters of the liver fluke Mediogonimus jourdanei Mas-Coma et Rocamora, 1978 were studied by means of transmission and scanning electron microscopy. Spermiogenesis begins with the formation of the differentiation zone containing two centrioles associated with striated rootlets and an intercentriolar body. These two centrioles originate two free flagella that undergo a 90° rotation before fusing with the median cytoplasmic process. Both nuclear and mitochondrial migrations toward the median cytoplasmic process occur before the proximodistal fusion of flagella. Finally, the constriction of the ring of arched membranes gives rise to the young spermatozoon. The mature sperm of M. jourdanei measures about 260 µm and presents two axonemes of different lengths with the typical pattern of the Trepaxonemata, two bundles of parallel cortical microtubules, one mitochondrion, a nucleus and granules of glycogen. An analysis of all the microphalloidean species studied to date emphasised some differences in certain characters found in Maritrema linguilla Jägerskiöld, 1908 and Ganeo tigrinum Mehra et Negi, 1928 in comparison to those in the remaining microphalloideans. The presence and variability of such ultrastructural characters according to family, superfamily or order have led several authors to propose their use in the analysis of trematode relationships and phylogeny. Therefore, apart from producing new data on the family Prosthogonimidae, the present study also compares the spermatological organization of M. jourdanei with other available ultrastructural studies focusing on the Microphalloidea.
Spermiogenesis and ultrastructure of mature spermatozoon of the caryophyllidean cestode Hunterella nodulosa, a parasite of suckers (Catostomidae), have been studied by transmission electron microscopy. This monozoic tapeworm is unique in its mode of attachment and represents the second North American species studied. The process of spermiogenesis of H. nodulosa follows the general pattern already described in other caryophyllideans. The most characteristic feature is the presence of a slight rotation of the flagellar bud, which seems to be a typical character of spermiogenesis in this cestode group. The mature spermatozoon of H. nodulosa is characterized by the presence of one axoneme of 9 + ''1'' type of the trepaxonematan flatworms surrounded by a semi-arc of cortical microtubules in its anterior extremity, parallel nucleus and cortical microtubules arranged in a parallel pattern, which corresponds to the Type III pattern of cestode spermatozoa according to Levron et al. (2010). Comparison of the present data with those available for other caryophyllideans did not reveal substantial differences, even though they belong to different families, infect different hosts (catostomid, cyprinid and siluriform fishes) and occur in distant zoogeographical regions. This indicates uniformity of the process of sperm formation and spermatozoon ultrastructure in one of the evolutionarily most ancient groups of tapeworms.
Spermiogenesis and the ultrastructural organisation of the spermatozoon of the trypanorhynch cestode Aporhynchus menezesi Noever, Caira, Kuchta et Desjardins, 2010 are described by means of transmission electron microscopy. Type I spermiogenesis of A. menezesi starts with the formation of a differentiation zone containing two centrioles separated by an intercentriolar body constituted by five electron-dense plates. Each centriole gives rise to a free flagellum, which grows at an angle of 90° in relation to a median cytoplasmic process. The nucleus and cortical microtubules elongate along the spermatid body. Later, both flagella rotate and fuse with the median cytoplasmic process. At the final stage of spermiogenesis, the young spermatozoon is detached from the residual cytoplasm by a narrowing of the ring of arched membranes. The mature spermatozoon is a long and filiform cell, tapered at both ends, lacking mitochondria. It is characterized by the presence of two axonemes of the 9+'1' trepaxonematan pattern, the absence of crested bodies, the presence of parallel cortical microtubules and nucleus. This pattern corresponds to the type I spermatozoon of the eucestodes. The anterior extremity of the spermatozoon is characterized by the presence of an arc-like row of up to seven parallel cortical microtubules that partially surrounds the first axoneme. These anterior cortical microtubules are thicker than the posterior microtubules and, consequently, the sperm cell of A. menezesi exhibits two types of cortical microtubules. Another interesting aspect is the presence of α-glycogen rosettes. This spermatological pattern is similar to that observed in the spathebothriidean and diphyllobothriidean cestodes.
Sphaeromyxa artedielli sp. n. is described from the gall bladder of the Atlantic hookear sculpin Artediellus atlanticus Jordan et Evermann (Cottidae; type host) from northern Norway. The parasite was also found to infect Triglops murrayi Günther (Cottidae). Spores are produced in disporic pansporoblasts in large flat plasmodia. Spores are straight and fusiform with truncated ends, and measure 16.5-18.7 µm × 4.9-6.2 µm. Valves are thick, striated and suture line is straight. Two equal ovoid polar capsules measure 4.2-6.8 µm × 2.9-4.4 µm and contain irregularly folded polar filaments. Distinctive features include spore shape and size, spore length/width relationship, striated valves, equal polar capsules and a short intercapsular distance. Sphaeromyxa bonaerensis Timi et Sardella, 1998, Sphaeromyxa cannolii Sears, Anderson et Greiner, 2011, and Sphaeromyxa sevastopoli Naidenova, 1970 produce straight spores with truncated ends that are of similar length as those of the new species. Sphaeromyxa cannolii differs in showing smooth spores with unequal polar capsules. The new species differs from S. bonaerensis and S. sevastopoli in significantly wider spores and polar capsules. Sphaeromyxa balbianii Thélohan, 1892, a species originally described with significantly smaller spores than S. artedielli sp. n., has previously been recorded from T. murrayi. We show that S. artedielli sp. n. differs from S. balbianii from the type host Gaidropsarus vulgaris (Cloquet) by its SSU rDNA sequence, and suggest that Atlantic records of Sphaeromyxa spp. from T. murrayi represent S. artedielli sp. n. The closest relative to S. artedielli sp. n. according to the SSU rDNA sequences, S. longa Dunkerly, 1921, differs clearly by spore size and shape. In the SSU rDNA-based phylogenetic analyses, S. artedielli sp. n. groups with other Sphaeromyxa spp. with straight spores and truncated ends in a clade that represents a sister-group to Sphaeromyxa spp. with arcuate spores and rounded ends. Our results indicate that an SSU rDNA pseudogene is present in S. balbianii.
The distribution of Sphaerospora dicentrarchi Sitjà-Bobadilla et Alvarez-Pellitero, 1992 and S. testicularis Sitjà-Bobadilla et Alvarez-Pellitero, 1990, myxozoan parasites of European seabass Dicentrarchus labrax (L.), was investigated in different farming systems in Italy. In total, 1406 fish were examined. High S. dicentrarchi prevalence was observed in all the farming systems involved in this survey (extensive farms: 51.5%; intensive farms: inland 59.6%, inshore floating cages 76.2%, offshore floating cages 41.6%) except for submersible cages (7.4%). S. testicularis was detected only in nine male fish from two intensive farms. The epidemiology and pathological effects of the parasites are discussed.
A new species of sphaerosporid myxosporean, Sphaerospora elwhaiensis sp. n., is described from kidney of non-anadromous sockeye salmon (kokanee) Oncorhynchus nerka (Walbaum) from Lake Sutherland in the northern Olympic Peninsula, Washington, USA. Infection with the parasite was detected in 45% of 177 kokanee examined over 5 years. While conforming to the morphological criteria by which members of the genus are defined, the parasite is distinguished from congeners in salmonids of western North America by a unique combination of valvular sculpting of the myxospore, the relatively large size of the myxospore and monosporous development within the pseudoplasmodium. In addition, nucleotide sequences of the parasite's small and large subunit ribosomal RNA gene are unique. Phylogenetic analyses of these sequences suggested that the parasite is most closely related to freshwater Myxidium spp. and Zschokkella spp. The molecular data have provided further evidence for a polyphyletic association previously recognized among members of the genus and emphasize the need for a taxonomic revision of Sphaerospora Thélohan, 1892 and related genera.