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302. Adenosine A1, A2a, A2b, and A3 receptors in hematopoiesis. 1. Expression of receptor mRNA in four mouse hematopoietic precursor cells

Creator:
Denisa Štreitová, Luděk Šefc, Filipp Geirgievich Savvulidi, Pospíšil, M., Jiřina Holá, and Michal Hofer
Format:
print
Type:
article, články, model:article, and TEXT
Subject:
Fyziologie člověka a srovnávací fyziologie, adenosinové receptory, krvetvorba, adenosine receptors, hematopoiesis, mRNA expression, 14, and 612
Language:
English
Description:
Four mouse bone marrow or thymus cell populations, namely granulopoietic/monocytopoietic, erythropoietic, B-lymphopoietic, and T-lymphopoietic precursor cells have been assayed by RTPCR technique for the presence and relative amounts of adenosine A1, A2a, A2b, and A3 receptor mRNA. It has been found that (i) all four populations studied express all four adenosine receptor subtypes, (ii) the A1 receptor is the least expressed in all populations studied, (iii) the A3 receptor is markedly expressed in the populations of granulopoietic/monocytopoietic and erythropoietic cells, (iv) the A2a receptor is markedly expressed in the populations of B-lymphopoietic and T-lymphopoietic cells, and v) the A2b receptor does not predominate in any of the precursor cells studied. Our data offer a new possibility for the assessment of the readiness of these cells to respond, by receptor-mediated mechanisms, to adenosine or its analogs present in the tissues as a result of endogenous processes and/or following their administration., D. Štreitová ... [et al.]., and Obsahuje bibliografii a bibliografické odkazy
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public

303. Adenosine A1, A2a, A2b, and A3 receptors in hematopoiesis. 2. Expression of receptor mRNA in resting and lipopolysaccharide-activated mouse RAW 264.7 macrophages

Creator:
Denisa Štreitová, Michal Hofer, Jiřina Holá, Antonín Vacek, and Pospíšil, M.
Format:
print
Type:
article, články, model:article, and TEXT
Subject:
Fyziologie člověka a srovnávací fyziologie, adenosinové receptory, adenosine receptors, macrophage, mRNA expression, 14, and 612
Language:
English
Description:
Expression of mRNA for adenosine receptor subtypes A1, A2a, A2b, and A3 in normal and lipopolysaccharide (LPS)-activated murine RAW 264.7 macrophages has been investigated using the method of quantitative real-time polymerase chain reaction. The results have shown a very low, unquantifiable expression of adenosine A1 receptor mRNA in both normal and LPS-activated macrophages. The other three adenosine receptor mRNAs have been found to be expressed at various but always quantifiable levels. Activation of the macrophages by LPS induced upregulation of the expression of adenosine receptor A2a and A2b mRNA, whereas the expression of adenosine receptor A3 mRNA was downregulated. Unstimulated macrophages exhibited a high expression of the A2b adenosine receptor mRNA. The findings are discussed from the point of view of the antiinflammatory and hematopoiesis-stimulating roles of the adenosine receptor signaling., D. Štreitová ... [et al.]., and Obsahuje bibliografii a bibliografické odkazy
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public

304. Adhesion, growth and differentiation of osteoblasts on surface-modified materials developed for bone implants

Creator:
Vandrovcová, M. and Lucie Bačáková
Format:
print
Type:
article, články, model:article, and TEXT
Subject:
Fyziologie člověka a srovnávací fyziologie, drsnost povrchu, kostní tkáň, tkáňové inženýrství, surface roughness, bone tissue, tissue engineering, surface roughness and topography, surface wettability, surface coating, surface patterning, osteogenic cells, bone tissue engineering, 14, and 612
Language:
English
Description:
This review briefly outlines the history and possibilities of bone reconstruction using various types of artificial materials, which allow interaction with cells only on the surface of the implant or enable ingrowth of cells inside the material. Information is also provided on the most important properties of bone cells taking part in bone tissue development, and on diseases and regeneration. The most common cell types used for testing cell-material interaction in vitro are listed, and the most commonly used approaches to this testing are also mentioned. A considerable part of this review is dedicated to the physical and chemical properties of the materi al surface, which are decisive for the cell-material interaction, and also to modifications to the surface of the material aimed at integrating it better with the surrounding bone tissue. Special attention is paid to the effects of nanoscale and microscale surface roughness on cell behaviour, to material surface patterning, which allows regionally-selective adhesion and growth of cells, an d also to the surface chemistry. In addition, coating the materials with bioactive layers is examined, particularly those create d by deposition of fullerenes, hybrid metal-fullerene composites, carbon nanotubes, nanocrystalline diamond films, diamond-like carbon, and nanocomposite hydrocarbon plasma polymer films enriched with metals., M. Vandrovcová, L. Bačáková., and Obsahuje bibliografii a bibliografické odkazy
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public

305. Adheze cévních a kostních buněk na umělé materiály vyvíjené pro tkáňové inženýrství

Creator:
Bačáková, Lucie, Nosková, Lenka, Filová, Elena, Švorčík, Václav, Koutná, Eva, Starý, Vladimír, Horník, Jakub, and Glogar, Petr
Format:
print, bez média, and svazek
Type:
model:article and TEXT
Subject:
biomedicína, tkáňové inženýrství, polyethylen, biomedicine, tissue engineering, polyethylene, 6, and 53
Language:
Czech
Description:
Adheze buněk na umělé materiály konstruované pro potenciální biomedicínské využití je regulována povrchovými fyzikálně-chemickými vlastnostmi těchto materiálů. Ozáření polyethylenu ionty O+ a C+ v této studii vedlo k formování chemických funkčních skupin s obsahem kyslíku na jeho povrchu, konjugovaných dvojných vazeb mezi atomy uhlíku, zvýšení povrchové smáčivosti a zvýšení adsorpce kolagenu IV tj. proteinu zprostředkujícího adhezi buněk. Cévní hladké svalové buňky potkana adherovaly k modifikovanému materiálu ve zvýšeném počtu, větší plochou a syntetizovaly více kontraktilních proteinů α-aktinu a SM-myosinů. Podobně i snížení povrchové drsnosti uhlíkových kompozitních materiálů, které jsou perspektivní pro konstrukci kostních náhrad, vedlo ke zvýšení jejich smáčivosti, adsorpce kolagenu IV a následné adheze, růstu a diferenciace lidských kostních buněk., Lucie Bačáková, Lenka Nosková, Elena Filová, Václav Švorčík, Eva Koutná, Vladimír Starý, Jakub Horník, Petr Glogar., and Obsahuje seznam literatury
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public

308. Adiponectin and resistin gene polymorphisms in patients with anorexia nervosa and obesity and its influence on metabolic phenotype

Creator:
Jarmila Křížová, Markéta Dolinková, Zdeňka Lacinová, Štěpán Sulek, Radka Doležalová, Jitka Housová, Jana Krajíčková, Denisa Haluzíková, Lenka Bošanská, Hana Papežová, and Martin Haluzík
Format:
print, bez média, and svazek
Type:
article, články, model:article, and TEXT
Subject:
Patologie. Klinická medicína, fyziologie člověka, polymorfismus, obezita, mentální anorexie, human physiology, polymorphism, obesity, anorexia nervosa, adiponectin, resistin, 14, and 616
Language:
English
Description:
Genes for adiponectin and resistin are candidate genes of insulin resistance and type 2 diabetes mellitus. The aim of our study was to determine the frequency of single nucleotide polymorphisms (SNP) 45T>G and 276G>T of the adiponectin gene and 62G>A and -180C>G of the resistin gene in patients with obesity (OB), anorexia nervosa (AN) and in control healthy normal-weight women (NW) and to study the influence of particular genotypes on serum concentrations of these hormones and on insulin sensitivity. Serum adiponectin, resistin, tumor necrosis factor alpha (TNF-alpha), insulin, cholesterol, glycated hemoglobin (HbA1c) and blood glucose levels were measured in 77 patients with OB, 28 with AN and 38 NW. DNA analysis was carried out by polymerase chain reaction with restriction analysis of PCR product. The presence of SNP ADP+276 G>T allele was accompanied by higher cholesterol levels in AN patients, higher adiponectin concentrations in OB patients and lower HbA1c levels in NW. SNP of the resistin gene 62G>A was associated with lower HbA1c in NW and higher cholesterol concentrations in OB group. The carriers of the minor G allele in the position -180 of the resistin gene within AN group had significantly higher BMI relative to non-carriers. We conclude that polymorphisms in adiponectin and resistin genes can contribute to metabolic phenotype of patients with obesity and anorexia nervosa., J. Křížová, M. Dolinková, Z. Lacinová, Š. Sulek, R. Doležalová, J. Housová, J. Krajíčková, D. Haluzíková, L. Bošanská, H. Papežová, M. Haluzík., and Obsahuje bibliografii a bibliografické odkazy
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public

309. Adiponectin as a potential marker of prostate cancer progression: studies in organ-confined and locally advanced prostate cancer

Creator:
Daniel Housa, Zdenka Vernerová, Jiří Heráček, Bohumír Procházka, Petr Čechák, Jitka Kuncová, and Martin Haluzík
Format:
print, bez média, and svazek
Type:
article, články, model:article, and TEXT
Subject:
Biochemie. Molekulární biologie. Biofyzika, lékařská biochemie, rakovina prostaty, imunohistochemie, medical biochemistry, prostate cancer, immunohistochemistry, adiponektin, adiponectin, 2, and 577
Language:
English
Description:
Serum levels of adiponectin were measured in patients with benign prostatic hyperplasia and prostate cancer of pT2 and pT3 stage. Adiponectin ELISA assay, immunohistochemistry, and selected metabolic and biochemical parameters measurement was performed in 25 patients with benign prostatic hyperplasia and 43 with prostate cancer (17 patients with organ-confined and 26 patients with locally advanced disease). Serum adiponectin levels did not differ between prostate benign hyperplasia and cancer clinical stage T2, but was significantly higher in pT3 relative to pT2 group (14.51± 4.92 vs. 21.41±8. 12, P = 0.003). Tissue immunohistochemistry showed enhanced staining in neoplastic prostate glands and intraepithelial neoplasia relative to benign prostatic hyperplasia without distinction between disease grade and stage. Serum adiponectin levels are higher in locally advanced relative to organ-confined prostate cancer and may thus serve as an auxiliary marker providing further improvement for discrimination between pT2 and pT3 stages., D. Housa, Z. Vernerová, J. Heráček, B. Procházka, P. Čechák, J. Kuncová, M. Haluzík., and Obsahuje bibliografii a bibliografické odkazy
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public

310. Adiponectin inhibits hyperlipidemia-induced platelet aggregation via attenuating oxidative/nitrative stress

Creator:
Wang, W.-Q., Zhang, H.-F., Gao, G.-X., Bai, Q.-X., Li, R., and Wang, X.-M.
Format:
print
Type:
article, články, model:article, and TEXT
Subject:
Fyziologie člověka a srovnávací fyziologie, hyperlipidémie, oxidační stres, hyperlipidemia, oxidative stress, adiponectin, platelet, aggregation, 14, and 612
Language:
English
Description:
Adiponectin acts as an endogenous antithrombotic factor. However, the mechanisms underlying the inhibition of platelet aggregation by adiponectin still remain elusive. The present study was designed to test whether adiponectin inhibits platelet aggregation by attenuation of oxidative/nitrative stress. Adult rats were fed a regular or high-fat diet for 14 weeks. The platelet was immediately separated and stimulated with recombinant full-length adiponectin (rAPN) or not. The platelet aggregation, nitric oxide (NO) and superoxide production, endothelial nitric oxide synthase (eNOS)/inducible NOS (iNOS) expression, and antioxidant capacity were determined. Treatment with rAPN inhibited hyperlipidemia- induced platelet aggregation (P<0.05). Interestingly, total NO, a crucial molecule depressing platelet aggregation and thrombus formation , was significantly reduced, rather than increased in rAPN-treated platelets. Treatment with rAPN markedly decreased superoxide production (-62 %, P<0.05) and enhanced antioxidant capacity (+38 %, P<0.05) in hyperlipidemic platelets. Hyperlipidemia-induced reduced eNOS phosphorylation and increased iNOS expression were significantly reversed following rAPN treatment (P<0.05, P<0.01, respectively). Taken together, these data suggest that adiponectin is an adipokine that suppresses platelet aggregation by enhancing eNOS activation and attenuating oxidative/nitrative stress including blocking iNOS expression and superoxide production., W.-Q. Wang ... [et al.]., and Obsahuje bibliografii a bibliografické odkazy
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public

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