Alveolar echinococcosis is a severe chronic helminthic disease that mimics slow-growing liver cancer. The immune evasion strategy of Echinococcus multilocularis Leuckart, 1863 remains poorly understood. The aim of this study was to investigate in vitro the impact of E. multilocularis vesicular fluid (Em-VF) on peripheral blood mononuclear cells (PBMC) and on natural killer (NK) cells. PBMC and NK cells were exposed to Em-VF (1 µg/ml) during six days. The effect of Em-VF was assessed on CD69, viability and proliferation, and on and transforming growth factor β (TGF-β), interferon γ (IFN-γ), interleukin 17 (IL-17) and interleukin 10, using flow cytometry and ELISA, respectively. Exposure to Em-VF had no bearing on PBMC's viability, proliferation and expression of CD69. In contrast, higher levels of IL-17 at day three and of TGF-β at day six were observed in PBMC supernatant after exposure to Em-VF (p < 0.05, Wilcoxon signed-rank test). Exposure to Em-VF induced a significant decrease of CD69 expression of NK cells at day three and a significant decrease of proliferation of NK cells at day six (p < 0.05, Wilcoxon signed-rank test). In contrast, NK cells viability and levels of cytokines did not vary significantly over Em-VF stimulation. Exposure to Em-VF had a significant bearing on activation and proliferation of NK cells. NK cells may play an important role in the immune response of the host against E. multilocularis., Anne-Pauline Bellanger, Valentine Mougey, Jean-René Pallandre, Houssein Gbaguidi-Haore, Yann Godet, Laurence Millon., and Obsahuje bibliografii
K tisku upravil za přispění vynikajících odborníků Jan Eckert, odborný hospodářský učitel v Litomyšli a majitel "České Hospodyně" vycházející v Chrudimi
Rove beetles (Staphylinidae) are used to explore the forces that shaped the terrestrial fauna on Læsø, a young ca. 3000 year old Danish oceanic island located in the Kattegat strait between mainland Denmark and Sweden. We compile a detailed list of species of rove beetles for Læsø (328 species) and the surrounding Danish and Swedish regions (altogether 1075 species), which includes a standardized inventory of their body sizes, and the habitat and microhabitat preference of each species. The composition of the fauna on Læsø and adjacent mainland regions points to North-Eastern Jutland as the main source of the rove beetles on Læsø. Although large beetles are more active and likely to disperse than small beetles, there is no bias towards large species on the island indicating that the sea separating Læsø from the mainland has not been a barrier for rove beetle dispersal. The statistical analysis of the habitat and microhabitat preferences of the species of the entire system studied has shown that Læsø, compared to the mainland areas, is distinctly more dominated by ecological generalists, especially by species adapted to ephemeral, temporary humid microhabitats. Presumably, the mosaic of mostly dry open habitats available on Læsø filters for species, that are able to populate these suboptimal habitats via patches of humid but ephemeral microhabitats. A comprehensive eco-faunistic dataset for the Staphylinidae compiled for this study is the first modern account of the rove beetle fauna on the Danish island of Læsø., Aslak K. Hansen, Mathias J. Justesen, Sebastian Kepfer-Rojas, David B. Byriel, Jan Pedersen, Alexey Solodovnikov., and Obsahuje bibliografii
Edge-colourings of graphs have been studied for decades. We study edge-colourings with respect to hereditary graph properties. For a graph G, a hereditary graph property P and l\geqslant 1 we define X{'_{P,l}} to be the minimum number of colours needed to properly colour the edges of G, such that any subgraph of G induced by edges coloured by (at most) l colours is in P. We present a necessary and sufficient condition for the existence of X{'_{P,l}} . We focus on edge-colourings of graphs with respect to the hereditary properties Ok and Sk, where Ok contains all graphs whose components have order at most k+1, and Sk contains all graphs of maximum degree at most k. We determine the value of X{'_{{S_k},l}}(G) for any graph G,k \geqslant 1, l\geqslant 1 and we present a number of results on X{'_{{O_k},l}}(G) ., Samantha Dorfling, Tomáš Vetrík., and Obsahuje seznam literatury
In this paper, we investigate a measure of similarity of graphs similar to the Ramsey number. We present values and bounds for g(n, l), the biggest number k guaranteeing that there exist l graphs on n vertices, each two having edit distance at least k. By edit distance of two graphs G, F we mean the number of edges needed to be added to or deleted from graph G to obtain graph F. This new extremal number g(n, l) is closely linked to the edit distance of graphs. Using probabilistic methods we show that g(n, l) is close to \frac{1} {2}\left( {\begin{array}{*{20}c} n // 2 // \end{array} } \right) for small values of l > 2. We also present some exact values for small n and lower bounds for very large l close to the number of non-isomorphic graphs of n vertices., Tomasz Dzido, Krzysztof Krzywdziński., and Obsahuje seznam literatury