The Human Frontier Science Program (HFSP) promotes fundamental research in the life sciences with special emphasis on novel and interdisciplinary research, international and in particular intercontintental collaboration and support for young investigators. One of the mechanisms of research support is the Young Investigator Grants Program. Vladimír Sychrovský from Institute of Organic Chemistry and Biochemistry AS CR obtained that grant for his project Probing the mechanism of the cleavage reaction in catalytic RNAs and will cooperate with scientists from Japan and USA. and Libuše Marková.
The effects of combined administration of two drugs elevating extracellular adenosine, namely dipyridamole (DP) and adenosine monophosphate (AMP), and granulocyte colony-stimulating factor (G-CSF) on hemopoietic stem cells in vivo were investigated. The experiments were performed on mice using the endogenous spleen colony formation in gamma-irradiated animals as an endpoint. The results have shown that DP and AMP act additively with G-CSF to enhance spleen colony formation and thus the erythroid repopulation of the spleen. These findings indicate that the signaling pathways of G-CSF and drugs elevating extracellular adenosine can interact at the level of primitive hemopoietic stem cells. The enhancement of hemopoiesis-stimulating effects of G-CSF by DP and AMP, which are low-priced and clinically available drugs, could improve the cost-effectiveness of the therapy with G-CSF., M. Hofer, M. Pospíšil, J. Netíková, V. Znojil, J. Vácha., and Obsahuje bibliografii