Bacillus Calmette-Guérin (BCG) is an attenuated Mycobacterium tuberculosis vaccine. We performed a series of co-infection experiments with BCG-Plasmodium chabaudi chabaudi Landau, 1965 AS using C57BL/6 mice to analyse whether BCG can affect the development of protective immunity to infection with Plasmodium spp. and the mechanism of this protection. We divided mice into four groups: BCG-inoculation 4 weeks prior to P. c. chabaudi AS infection (B-4w-Pc); simultaneous BCG-inoculation and P. c. chabaudi AS infection (Pc+B); BCG-inoculation 3 days post P. c. chabaudi AS (Pc-3-B) infection; and mono-P. c. chabaudi AS infection as control (Pc). The parasitemia level in the B-4w-Pc group was noticeably higher than control group at 6-19 days post infection (dpi). Compared with the control group, the proportion of CD4+CD69+ T cells was significantly reduced 5, 8 and 12 dpi, but the proportion of CD4+CD25+Foxp3+ Tregs was significantly increased in the B-4w-Pc group on 5 and 8 dpi. The B-4w-Pc group also demonstrated reduced levels of IFN-γ and TNF-α on 5 and 8 dpi and significantly elevated level of IL-10 on 12 dpi. There were significantly fewer mDCs (CD11c+CD11b+) and pDCs (CD11c+B220+) in the B-4w-Pc group than the control group at all the time points post infection and the expression of MHC II was noticeably reduced on day 8 pi. Our findings confirmed that BCG inoculation prior to Plasmodium infection resulted in excessive activation and proliferation of Tregs and upregulation of anti-inflammatory mediators, which inhibited establishment of a Th1-dominant immune response during the early stages of Plasmodium infection by inhibiting dendritive cells response. BCG inoculation prior to P. c. chabaudi AS infection may contribute to overgrowth of parasites as well as mortality in mice., Dong-Hua Cao, Ji-Chun Wang, Jun Liu, Yun-Ting Du, Li-Wang Cui, Ya-Ming Cao., and Obsahuje bibliografii
Each cell types or tissues contain certain “physiological” levels of R-2-hydroxyglutarate (2HG), as well as enzymes for its synthesis and degradation. 2HG accumulates in certain tumors, possessing heterozygous point mutations of isocitrate dehydrogenases IDH1 (cytosolic) or IDH2 (mitochondrial) and contributes to strengthening their malignancy by inhibiting 2-oxoglutaratedependent dioxygenases. By blocking histone de-methylation and 5-methyl-cytosine hydroxylation, 2HG maintains cancer cells de-differentiated and promotes their proliferation. However, physiological 2HG formation and formation by non-mutant IDH1/2 in cancer cells were neglected. Consequently, low levels of 2HG might play certain physiological roles. We aimed to elucidate this issue and found that compared to highest 2HG levels in hepatocellular carcinoma HepG2 cells and moderate levels in neuroblastoma SH-SY5Y cells, rat primary fibroblast contained low basal 2HG levels at early passages. These levels increased at late passage and likewise 2HG/2OG ratios dropped without growth factors and enormously increased at hypoxia, reaching levels compared to cancer HepG2 cells. Responses in SH-SY5Y cells were opposite. Moreover, external 2HG supplementation enhanced fibroblast growth. Hence, we conclude that low 2HG levels facilitate cell proliferation in primary fibroblasts, acting via hypoxia-induced factor regulations and epigenetic changes., A. Dvořák, J. Zelenka, K. Smolková, L. Vítek, P. Ježek., and Obsahuje bibliografii
Knihovna Národního muzea Praha CZ 53 C 34, Královská kanonie premonstrátů na Strahově - Strahovská knihovna Praha CZ BX VI 30/19, Královská kanonie premonstrátů na Strahově - Strahovská knihovna Praha CZ BU II 132/37 def., Královská kanonie premonstrátů na Strahově - Strahovská knihovna Praha CZ BU II 133/21, Národní knihovna ČR Praha CZ 65 E 3387 def., Národní knihovna ČR Praha CZ 46 C 181 adl. 12, Klášter dominikánů - knihovna Praha CZ E III 137, Klášter Rytířského řádu křižovníků s červenou hvězdou - knihovna Praha CZ XV G 4 adl. 8, Knihovna Akademie věd ČR Praha CZ TF 347 adl. 23 def., Vědecká knihovna v Olomouci Olomouc CZ 602.419, Vědecká knihovna v Olomouci Olomouc CZ 602.271 adl. 4, and BCBT07614
Let X be a complex L1-predual, non-separable in general. We investigate extendability of complex-valued bounded homogeneous Baire-α functions on the set ext B_{X*} of the extreme points of the dual unit ball B_{X*} to the whole unit ball B_{X*}. As a corollary we show that, given α \in [1, ω1), the intrinsic α-th Baire class of X can be identified with the space of bounded homogeneous Baire-α functions on the set ext B_{X*} when ext B_{X*} satisfies certain topological assumptions. The paper is intended to be a complex counterpart to the same authors’ paper: Baire classes of non-separable L1-preduals (2015). As such it generalizes former work of Lindenstrauss and Wulbert (1969), Jellett (1985), and ourselves (2014), (2015)., Pavel Ludvík, Jiří Spurný., and Obsahuje seznam literatury