The presence of adipokinetic activity in crude extracts of corpora cardiaca (CC) from the butterfly (Vanessa cardui L., Nymphalidae) was demonstrated by bioassay and Mas-AKH was revealed as the major adipokinetic hormone (AKH) by use of two different technologies of sequence elucidation: HPLC separation of the peptide followed by Edman degradation and Q-TOF mass spectrometry. In contrast to the time- and material-consuming conventional methods of peptide purification and sequencing, substantial structural data of the peptide were confirmed - post factum - from one pCC (pair of CC) by Q-TOF mass spectrometry. Only males of our laboratory colony showed a significant lipid increase in the haemolymph after injection of either crude CC extract (1 pCC equivalent) or 10 pmol of synthetic peptide., Gabriele Köllisch, Peter D. Verhaert, Matthias W. Lorenz, Roland Kellner, Gerd Gäde, Klaus H. Hoffmann, and Lit
In order to manage the risks posed to domestic crop production by quarantine pests such as Thrips palmi, their potential to establish in a new environment must be assessed. The thermal requirements for development of T. palmi were determined and compared with UK temperatures, to estimate its potential for development under UK conditions. Temperature and rate of development of T. palmi from egg to adult were linearly related between 15 and 30°C, allowing calculation of an overall threshold of 10.1°C, and a sum of effective temperatures of 194 degree-days. In the UK, development of T. palmi would be possible outdoors during the summer when a maximum of up to four or five generations could occur. Comparison of these data with those of the recently established and biologically similar pest, Frankliniella occidentalis, shows that establishment of T. palmi in the UK is unlikely to be limited by an inability to complete the life cycle during the favourable season., Jamie R. McDonald, Jeffrey S. Bale, Keith F.A. Walters, and Lit
The male of Tetanoptera leucodactyla Verbèke, a genus and species of Sciomyzidae known until now only from one female specimen from the Congo, is described, and the genus is more fully characterized. The external morphology is illustrated and the male genitalia are described and figured for the first time. The relationships of Tetanoptera to genera of the Sepedon group and certain other genera of Tetanocerini are discussed. Tetanoptera appears to be related to Verbekaria, and intermediate in an evolutionary lineage between the relatively primitive Tetanocera and the advanced Thecomyia., Lloyd V. Knutson, Jean-Claude Vala, and Lit
Four species new to science are described from Vanuatu, namely Holophygdon nishidae sp. n., Idiocysta vanuatuensis sp.n., Nesocypselas minicysta sp. n., and Tingis parvoroe sp. n. New records are provided for six species, five of them being new to Vanuatu: Cysteochila idonea Drake, Stephanitis subfasciata Horváth, Teleonemia scrupulosa Stål, Tingis chazeaui Guilbert, and Tingis irregularis (Montrouzier). A key to genera and species of Vanuatu is added., Eric Guilbert, and Lit