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11. Structure elucidation of Mas-AKH as the major adipokinetic hormone in the butterfly Vanessa cardui (Lepidoptera: Nymphalidae)

Creator:
Gäde, Gerd, Hoffmann, Klaus H., Köllisch, Gabriele, Verhaert, Peter D., Lorenz, Matthias W., and Kellner, Roland
Format:
print, text, and regular print
Type:
article, bibliography, model:article, and TEXT
Subject:
zoologie, entomologie, hmyz, motýli, Nymphalidae, Vanessa cardui, corpora cardiaca, adipokinetické hormony, struktura, peptidické složení, hmotnostní spektrometrie, 595.2/.7, and 591.1
Language:
English
Description:
The presence of adipokinetic activity in crude extracts of corpora cardiaca (CC) from the butterfly (Vanessa cardui L., Nymphalidae) was demonstrated by bioassay and Mas-AKH was revealed as the major adipokinetic hormone (AKH) by use of two different technologies of sequence elucidation: HPLC separation of the peptide followed by Edman degradation and Q-TOF mass spectrometry. In contrast to the time- and material-consuming conventional methods of peptide purification and sequencing, substantial structural data of the peptide were confirmed - post factum - from one pCC (pair of CC) by Q-TOF mass spectrometry. Only males of our laboratory colony showed a significant lipid increase in the haemolymph after injection of either crude CC extract (1 pCC equivalent) or 10 pmol of synthetic peptide., Gabriele Köllisch, Peter D. Verhaert, Matthias W. Lorenz, Roland Kellner, Gerd Gäde, Klaus H. Hoffmann, and Lit
Rights:
http://creativecommons.org/publicdomain/mark/1.0/ and policy:public