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412. K13 propeller domain mutations and pfmdr1 amplification in isolates of Plasmodium falciparum collected from Thai-Myanmar border area in 2006-2010

Creator:
Phompradit, Papichaya, Chaijaroenkul, Wanna, Muhamad, Phunuch, and Na-Bangchang, Kesara
Format:
print, počítač, and online zdroj
Type:
model:article and TEXT
Subject:
malárie, molekulární markery, malaria, molecular markers, falciparum malaria, artemisinin resistance, PF3D7_1343700, sequencing, 2, and 59
Language:
English
Description:
The K13 propeller domain mutation and pfmdr1 amplification have been proposed as useful molecular markers for detection and monitoring of artemisinin resistant Plasmodium falciparum Welch, 1897. Genomic DNA isolates of P. falciparum was extracted from 235 dried blood spot or whole blood samples collected from patients with uncomplicated falciparum malaria residing in areas along the Thai-Myanmar border during 2006-2010. Nested polymerase chain reaction (PCR) and sequencing were performed to detect mutations in K13 propeller domain of P. falciparum at codon 427-709. Pfmdr1 gene copy number was determined by SYBR Green I real-time PCR. High prevalence of pfmdr1 multiple copies was observed (42.5% of isolates). The presence of K13 mutations was low (40/235, 17.2%). Seventeen mutations had previously been reported and six mutations were newly detected. The C580Y was found in two isolates (0.9%). The F446I, N458Y and P574L mutations were commonly detected. Seven isolates had both K13 mutation and pfmdr1 multiple copies. It needs to be confirmed whether parasites harbouring both K13 mutation and pfmdr1 multiple copies and/or the observed new mutations of K13 propeller domain are associated with clinical artemisinin resistance., Papichaya Phompradit, Wanna Chaijaroenkul, Phunuch Muhamad, Kesara Na-Bangchang., and Obsahuje bibliografii
Rights:
http://creativecommons.org/publicdomain/mark/1.0/ and policy:public

413. Karas stříbřitý a jeho příbuzní

Creator:
Lukáš Kalous
Type:
article, články, journal articles, model:article, and TEXT
Subject:
Zoologie, zoologie, ryby, obojživelníci, plazi, molekulární biologie, virologie, imunologie, fyziologie, genetika, fishes, amphibians, reptiles, molecular biology, virology, immunology, physiology, genetics, 2, and 59
Language:
Czech
Description:
Článek se zabývá taxonomií, reprodukční biologií, fylogenetikou, fylogeografií a introdukční historií ryb rodu karas (Carassius). Článek poskytuje souhrn poznatků, které jsou značně komplikované a spojené s jedinečným způsobem rozmnožování, definicí druhu a nepřesným chápáním biologie a systematiky těchto ryb v minulosti. Celá problematika je ještě daleko od svého kompletního vyřešení, ale současný pohled nám může pomoci v lepší péči o vodní prostředí., This article deals with the taxonomy, re­productive biology, phylogenetics, phylo­geography and introduction history of the fishes of the genus Carassius. It summarizes findings associated with their unique mode of reproduction, challenging species delimitation and insufficient understanding of the biology and systematics of these fishes in the past. Deeper insight into the biology of Prussian Carp (Carassius gibelio) will require more research, but the available re­sults can improve our understanding and management of the aquatic environment., Lukáš Kalous., and Obsahuje seznam literatury
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public

416. Karyotypes of central European spiders of the genera Arctosa, Tricca, and Xerolycosa (Araneae: Lycosidae)

Creator:
Dolejš, Petr, Kořínková, Tereza, Musilová, Jana, Opatová, Věra, Kubcová, Lenka, Buchar, Jan, and Král, Jiří
Type:
article, články, model:article, and TEXT
Subject:
Zoologie, entomologie, Česko, Rakousko, Czechia, Austria, Lycosidae, spiders, karyotype, sex chromosomes, meiosis, constitutive heterochromatin, nucleolar organizer region, 2, and 59
Language:
English
Description:
The aim of this study was to characterize karyotypes of central European spiders of the genera Arctosa, Tricca, and Xerolycosa (Lycosidae) with respect to the diploid chromosome number, chromosome morphology, and sex chromosomes. Karyotype data are reported for eleven species, six of them for the first time. For selected species the pattern in the distributions of the constitutive heterochromatin and the nucleolar organizer regions (NORs) was determined. The silver staining technique for detecting NORs of lycosid spiders was standardized. The male karyotype consisted of 2n = 28 (Arctosa and Tricca) or 2n = 22 (Xerolycosa) acrocentric chromosomes. The sex chromosome system was X1X20 in all species. The sex chromosomes of T. lutetiana and X. nemoralis showed unusual behaviour during late diplotene, namely temporary extension due to decondensation. C-banding technique revealed a small amount of constitutive heterochromatin at the centromeric region of the chromosomes. Two pairs of autosomes bore terminal NORs. Differences in karyotypes among Arctosa species indicate that the evolution of the karyotype in this genus involved autosome translocations and size changes in the sex chromosomes. Based on published results and those recorded in this study it is suggested that the ancestral male karyotype of the superfamily Lycosoidea consisted of 28 acrocentric chromosomes. and Petr DOLEJŠ, Tereza KOŘÍNKOVÁ, Jana MUSILOVÁ, Věra OPATOVÁ, Lenka KUBCOVÁ, Jan BUCHAR, Jiří KRÁL.
Rights:
http://creativecommons.org/publicdomain/mark/1.0/ and policy:public

419. Ke studiu půdní semenné banky

Creator:
Kůrová, Jana
Type:
article, články, journal articles, model:article, and TEXT
Subject:
Zoologie, zoologie, botanika, fyziologie rostlin, pěstitelství, životní prostředí, ekologie, botany, plant physiology, horticultural education, environment, 2, and 59
Language:
Czech
Description:
Půdní semenná banka má vliv na složení vegetace na daném stanovišti. Semena různých druhů rostlin jsou velmi variabilní jak svým tvarem, tak velikostí. Tato variabilita výrazně ovlivňuje vznik a vývoj složení půdní semenné banky a tím i životnost semene. Složení půdní semenné banky je často výrazně odlišné od složení vegetace na daném stanovišti, což je nejvíce patrné u lesních biotopů. Ke studiu půdní semenné banky se využívá zejména dvou rozdílných metod - metoda ruční separace semen a kultivační metoda., The soil seed bank influences the composition of vegetation on the site. Seeds of different plant species are highly variable both in shape and size. This variation strongly affects the formation and composition of soil seed bank, as well as seed persistence. Soil seed bank composition often considerably differs from that of the local vegetation, which is most evident in forest habitats. Two different methods - separation and cultivation - are used for research into the soil seed bank., Jana Kůrová., and Obsahuje seznam literatury
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public

420. Key factors affecting the predation risk on insects on leaves in temperate floodplain forest

Creator:
Drozdová, Michaela, Šipoš, Jan, and Pavel Drozd
Type:
article, články, model:article, and TEXT
Subject:
Zoologie, zoologie, entomologie, Diptera, Calliphoridae, Calliphora vicina, insect predators, living prey, temporal and spatial differences, clumped dispersal of attacks, 2, and 59
Language:
English
Description:
The predation on insects on leaves was measured by direct observation using live larvae of Calliphora vicina (Diptera: Calliphoridae) as bait placed on 15 common species of woody plants in a floodplain forest in the temperate region. The predation rate was measured in terms of the proportion of the larvae that were missing or had been attacked after 30 min of exposure on leaves. Despite the fact that the important predators differed during the course of a season, the most frequently recorded predators were birds and ants and less frequently recorded wasps and spiders. Analysis of the pattern in the distribution of the attacks confirmed that it is best described by a negative binomial distribution, which corresponds to a clumped dispersal of predation. Based on the results of the best-fitted generalized additive model, we could not reject an equal probability of attacks on the different species of woody plants. Further, predation at the forest edge was notably higher than in the forest interior. The model also predicted marked variations in the incidence of attacks during the course of a day and a season, with the attacks occurring mainly in three periods during the year and two during the day. In general, the sampling method used could become the standard measure of the risk of insects living on trees being attacked by predators in future studies., Michaela Drozdová, Jan Šipoš, Pavel Drozd., and Obsahuje seznam literatury
Rights:
http://creativecommons.org/publicdomain/mark/1.0/ and policy:public

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