The development of acute respiratory distress syndrome (ARDS) is known to be independently attributable to aspiration-induced lung injury. Mechanical ventilation as a high pressure/volume support to maintain sufficient oxygenation of a patient could initiate ventilator-induced lung injury (VILI) and thus contribute to lung damage. Although these phenomena are rare in the clinic, they could serve as the severe experimental model of alveolar-capillary membrane deterioration. Lung collapse, diffuse inflammation, alveolar epithelial and endothelial damage, leakage of fluid into the alveoli, and subsequent inactivation of pulmonary surfactant, leading to respiratory failure. Therefore, exogenous surfactant could be considered as a therapy to restore lung function in experimental ARDS. This study aimed to investigate the effect of modified porcine surfactant in animal model of severe ARDS (P/F ratio ≤13.3 kPa) induced by intratracheal instillation of hydrochloric acid (HCl, 3 ml/kg, pH 1.25) followed by VILI (VT 20 ml/kg). Adult rabbits were divided into three groups: untreated ARDS, model treated with a bolus of poractant alfa (Curosurf®, 2.5 ml/kg, 80 mg phospholipids/ml), and healthy ventilated animals (saline), which were oxygen-ventilated for an additional 4 h. The lung function parameters, histological appearance, degree of lung edema and levels of inflammatory and oxidative markers in plasma were evaluated. Whereas surfactant therapy with poractant alfa improved lung function, attenuated inflammation and lung edema, and partially regenerated significant changes in lung architecture compared to untreated controls. This study indicates a potential of exogenous surfactant preparation in the treatment of experimental ARDS.
The present article describes two unrelated cases of progressive myoclonic epilepsy (PME) of the Lafora's disease and Unverricht-Lundborg types who were treated with topiramate (TPM) as add-on therapy for their myoclonus. After the initiation of topiramate therapy both cases responded with marked decrease in myoclonic seizure frequency and improvement of quality of life. Topiramate appears to be a useful alternative agent in cases of PME and could be consider for adjunctive therapy. and CF. Demir, HH. Ozdemir, B. Müngen
Data Envelopment Analysis (DEA) is a beneficial mathematical programming method to measure relative efficiencies. In conventional DEA models, Decision Making Units (DMUs) are usually considered as black boxes. Also, the efficiency of DMUs is evaluated in the presence of the specified inputs and outputs. Nevertheless, in real-world applications, there are situations in which the performance of multi-stage processes like supply chains with forward and reverse flows must be measured such that some of the intervening factors, called proportional dual-role factors, are presented that one part of each proportional dual-role factor plays the input role and the other plays the output role. To address this issue, the current study proposes radial and non-radial DEA models for evaluating the overall and stage efficiencies of the closed-loop supply chains when there are proportional dual-role factors. To illustrate, a proportional dual-role factor is divided into portions of the input of the first stage and the output of the second stage such that the optimal overall and stage efficiency scores of closed-loop supply chain are obtained. A case study is used to illustrate the proposed approach. The experimental results obtained from real world data show the convincing performance of our proposed method.
A good knowledge of hydraulic characteristics, particularly the retention curves, of the applied materials is necessary when studying the efficiency of a capillary barrier or numerically simulating flow of water in its layers. The main difficulties of their measurements originate in the fact that the coarse-grained soils of capillary barriers come from the state of residual moisture to the state of saturation within relatively small interval of pressure head. Hence, it is necessary to carry out the measurements in very fine steps of pressure head. Four materials of two capillary barriers are investigated in this paper. The tension apparatus according to Havlíček and Myslivec was used to measure their retention curves. Both branches of retention curves were measured in order to obtain data suitable for taking hysteresis into account. The method is described and the results are presented. Subsequently, the hydraulic characteristics of the materials are determined and their impact to the capillary barriers efficiency is investigated. The barriers were compared from the viewpoint of their efficiency using the measured data and tipping trough measurements made elsewhere. Explanation of the found differences was suggested and the effect of hysteresis was discussed., Dagmar Trpkošová and Jiří Mls., and Obsahuje bibliografii
We study the efficiency of individual stations of the CRL seismic network in recording the seismic activity in the western Gulf of Corinth, Greece. The stations are located on both the northern and southern coast of the Gulf. The study is based on 5027 earthquakes recorded in 2001, separated into three groups, the southern, central and the northern one. The events were located using the HYPO71PC algorithm. It is shown that the stations significantly differ in their monitoring ability., Jaromír Janský, Jiří Zahradník and Vladimír Plicka., and Obsahuje bibliografii
Two exogenous NO donors were used to act as substitutes for impaired endogenous nitric oxide (NO) production due to inhibition of NO synthase in rats. Six weeks' lasting inhibition of NO synthase by NG-nitro-L-arginine methyl ester (L-NAME) induced stabilized hypertension. Simultaneously administered isosorbide-5-mononitrate did not prevent the development of hypertension. Molsidomine, administered concomitantly with L-NAME, significantly attenuated the BP increase. However, BP was still found to be moderately increased compared to the initial values. Remarkable alterations in the geometry of the aorta, carotid and coronary artery found in NO-deficient hypertension were prevented in rats administered L-NAME plus molsidomine at the same time. In spite of 6 weeks' lasting inhibition of NOS, the NOS activators acetylcholine and bradykinin induced BP decrease; the maximum hypotensive value did not differ from the values recorded in the controls or in animals treated with L-NAME plus molsidomine. Notably enough, the hypotension was similar to that found in rats administered L-NAME alone for six weeks. After NO synthase inhibition, Isosorbide-5-mononitrate does not substitute and molsidomine substitute only partially the impaired endogenous NO production., M. Gerová, F. Kristek., and Obsahuje bibliografii
Pitfall traps are widely used for sampling ground-dwelling arthropods. Their sampling efficiency is affected by several factors, e.g. material, size and modification of parts of the trap and sampling design. Pitfall trap sampling is also affected by the accumulation of plant litter in the traps, rain fall and by-catches of small vertebrates, which may cause a bias in the catch by obstructing traps or attracting certain insects. A roof that prevents rain and plant litter entering a trap, prevents dilution of the preservative and escape of arthropods. The main goal of present study was to compare the effect of four types of differently combined funnel and roof pitfall traps on the capture efficiency of epigeal arthropods. We found that a funnel and/or a roof had no effect on spider catches. Total abundance of large carabids and thus the total abundance of ground beetles was lower in funnel pitfall traps without a roof than in other types of traps. However, funnel pitfall traps with roofs collected significantly more carabid beetles, especially individuals of those species that are large or good fliers. We conclude that funnel pitfall traps with roofs have no negative effects on capture efficiency of ground beetles and spiders, therefore application of this sampling technique is strongly recommended.