Hypoxia stimulates ventilation, but when it is sustained, a decline in the ventilatory response is seen. The mechanism responsible for this decline lies within the CNS, but still remains unknown. In this study, we attempted to elucidate the possible role of hypoxia-induced depression of respiratory neurons by comparing the ventilatory response to hypoxia in intact rats and those with denervated carotid bodies. A whole-body plethysmograph was used to measure tidal volume, frequency of breathing and minute ventilation (VE) in awake and anesthetized intact rats and rats after carotid body denervation during exposure to hypoxia (FIO2 0.1). Fifteen-minute hypoxia induced an initial increase of VE in intact rats (to 248 % of control ventilation in awake and to 227 % in anesthetized rats) followed by a consistent decline (to 207 % and 196 % of control VE, respectively). Rats with denervated carotid bodies responded with a smaller increase in VE (to 134 % in awake and 114 % in anesthetized animals), but without a secondary decline (145 % and 129 % of control VE in the 15th min of hypoxia). These results suggest that afferentation from the carotid bodies and/or the substantial increase in ventilation are crucial for the biphasicity of the ventilatory response to sustained hypoxia and that a central hypoxic depression cannot fully explain the secondary decline in VE., H. Maxová, M. Vízek., and Obsahuje bibliografii
Ventricular assist devices (VAD ) have recently established themselves as an irreplaceable th erapeutic modality of terminal heart failure. Because of the worldwide shortage of donors, ventricular assist devices play a key role in modern heart failure therapy. Some clinical data have revealed the possibility of cardiac recovery during VAD applic ation. On the other hand, both clinical and experiment al studies indicate the risk of the cardiac atrophy development, especially after prolonged mechanical unloading. Little is known about the specific mechanisms governing the unloading-induced cardiac atrophy and about the exact ultrastructural changes in cardiomyocytes, and even less is known about the ways in which possible therapeutical interventions may affect heart at rophy. One aim of this review was to present important aspects of the development of VAD- related cardiac atrophy in humans and we also review the most significant observations linking clinical data and those derived from studies using experimental mo dels. The focus of this article was to review current methods applied to alleviate cardiac atrophy which follows mechanical unloading of the heart. Out of many pharmacological agents studied, only the selective beta2 agonist clenbuterol has been proved to have a significantly beneficial effect on unloading-induced atrophy. Mechanical means of atrophy alleviation also seem to be effective and promising., M. Pokorný ... [et al.]., and Obsahuje bibliografii a bibliografické odkazy
Aims of the study were to compare the development of electrocardiographic responses of the ischemia-induced heterogeneities of activation and repolarization in the ventricular myocardium of normal and diabetic animals. Body surface ECGs and unipolar electrograms in 64 epicardial leads were recorded before and during 20 min after the ligation of the left anterior descending artery in diabetic (alloxan model, 4 weeks, n=8) and control (n=8) rabbits. Activation times (ATs), end of repolarization times (RTs) and repolarization durations (activation-recovery intervals, ARIs) were determined in ischemic and periischemic zones. In contrast to the controls, the diabetic rabbits demonstrated the significant prolongation of ATs and shortening of ARIs (P<0.05) during ischemia in the affected region resulting in the development and progressive increase of the ARI and RT gradients across the ischemic zone boundary. The alterations of global and local dispersions of the RTs in diabetics correlated with the Tpeak-Tend interval changes in the limb leads ECGs. In the ischemic conditions, the diabetic animals differed from the controls by the activation delay, significant repolarization duration shortening, and the increase of local repolarization dispersion; the latter could be assessed by the Tpeak-Tend interval measurements in the body surface ECGs., K. A. Sedova, M. A. Vaykshnorayte, A. O. Ovechkin, P. Kneppo, O. G. Bernikova, V. A. Vityazev, J. E. Azarov., and Obsahuje bibliografii
This work deals with the changes of rhetorical education and emotional orders in the second half of the 18th century. The aim of the research is to assess the relations among language education, funtions of medias, anthropological models and expression of emotions on the Threshold of enlightenment. The background of the research shapes the transformation of rhetorical tradition. The research of the broad field of pedagogical, rhetorical and moral discurs is focused on the collegium of Karl Heinrich Seibt., Václav Smyčka., and Obsahuje bibliografické odkazy
Článek se zabývá vývojem veřejného zdravotnictví jako oboru od jeho počátků koncem 18.století až po současnost. Jsou zmíněny některé důležité trendy a faktory, které teorii i praxi oboru ovlivnily, včetně zmínky o některých významných, našich i zahraničních protagonistech, kteří se o rozvoj veřejného zdravotnictví zasloužili. Je stručně zmíněn celoevropský kontext, s důrazem na skutečnosti, které současnou podobu veřejného zdravotnictví u nás nejvíce poznamenaly., The article is dealing with a development of the public health from its beginning at the end of the 18th century to the present. Some of the most important trends and factors influencing theory and practice of public health are briefly outlined. Some of the international and local protagonists which contributed the most are mentioned. Relevant European context of the public health development is also shortly discussed., Helena Hnilicová, and Lit.: 21
We verify functional a posteriori error estimate for obstacle problem proposed by Repin. Simplification into 1D allows for the construction of a nonlinear benchmark for which an exact solution of the obstacle problem can be derived. Quality of a numerical approximation obtained by the finite element method is compared with the exact solution and the error of approximation is bounded from above by a majorant error estimate. The sharpness of the majorant error estimate is discussed.
We verify functional a posteriori error estimates proposed by S. Repin for a class of obstacle problems in two space dimensions. New benchmarks with known analytical solution are constructed based on one dimensional benchmark introduced by P. Harasim and J. Valdman. Numerical approximation of the solution of the obstacle problem is obtained by the finite element method using bilinear elements on a rectangular mesh. Error of the approximation is measured by a functional majorant. The majorant value contains three unknown fields: a gradient field discretized by Raviart-Thomas elements, Lagrange multipliers field discretized by piecewise constant functions and a scalar parameter β. The minimization of the majorant value is realized by an alternate minimization algorithm, whose convergence is discussed. Numerical results validate two estimates, the energy estimate bounding the error of approximation in the energy norm by the difference of energies of discrete and exact solutions and the majorant estimate bounding the difference of energies of discrete and exact solutions by the value of the functional majorant.
The article presents results of the verification calculation of the method for the calculation of natural frequencies and mode shapes of a linear undamped rotationally periodic systems considering the possibility of the elimination of degrees of freedom. As the test example a thin circular plate was chosen. The method can be applied e.g. for the calculation of the natural vibration characteristics of the steam turbine bladed disks. and Obsahuje seznam literatury