2’(3’)-0-[N- [2- [3- [5-fluoresceinyl] thioureido] ethyl] carbamoyl] adenosine 5’-triphosphate (FEDA-ATP), a spectroscopic tool used for studying skeletal muscle myosin ATPase subfragment 1, was applied to Na+/K+- ATPase (EC 3.6.1.37). In contrast to the myosin subfragment, we found that FEDA-ATP is not a substrate for Na + /K + -ATPase. On the other hand, FEDA-ATP showed an affinity for both the low (E2, K<j = 2001MM) and the high (Ei, Kd = 22,«M) affinity ATP-binding sites. When the microscopic affinities of FEDA-ATP were used for calculating the macroscopic affinity in the overall reaction according to Kj = (KdEl*KdE2)1/2, the experimentally measured inhibition constant of 66,wM was obtained. To evoke irreversible binding inhibitors, FEDA-ATP was transferred in its chromium (III) and cobalt(III) complex analogs, which are suitable tools for labelling the ATP binding sites of Na + /K+ -ATPase in a specific way.
Odlupováním vrstevnatých materiálů (např. grafitu) lze připravit 2D krystaly s tloušťkou jednoho nebo několika málo atomů. Nejznámějším takovým 2D materiálem je grafen (monovrstva grafitu), nicméně v současnosti existují a intenzivně se zkoumají desítky podobných struktur. Příspěvek podává základní přehled o 2D materiálech, jejich vlastnostech, metodách přípravy a možných aplikacích., Layered materials (such as graphite) can be exfoliated to produce 2D crystals with a thickness of one of just a few atoms. The best known 2D material is graphene (monolayer of graphite), yet there are tens of similar structures, which are being studied intensively at the moment. This article presents a brief summary of 2D materials, their properties, methods of preparation and potential application., Zdeňka Hájková, Martin Ledinský, Matěj Hývl, Aliaksei Vetushka, Antonín Fejfar, Jaroslava Řáhová, Otakar Frank., and Obsahuje bibliografické odkazy