Hypoxic pulmonary vasoconstric tion (HPV) is an important homeostatic mechanism in which increases of [Ca2+] i are primary events. In this study, primary cultured, human pulmonary artery smooth muscle cells (hPASMC) were used to examine the role of TRPC channels in mediating [Ca2+] i elevations during hypoxia. Hypoxia (PO2 about 20 mm Hg) evoked a transient [Ca2+] i elevation that was reduced by removal of extracellular calcium. Nifedipine and verapamil, blockers of vo ltage-gated calcium channels (VGCCs), attenuated th e hypoxia-induced [Ca2+] i elevation by about 30 %, suggesting the presence of alternate Ca2+ entry pathways. Expression of TRPC1 an d TRPC6 in hPASMC were found by RT-PCR and confirmed by Western blot analysis. Antagonists for TRPC, 2APB and SKF96365, significantly reduced hypoxia-induced [Ca2+] i elevation by almost 60 %. Both TRPC6 and TRPC1 were knocked down by siRNA, the loss of TRPC6 decreased hypoxic response down to 21 % of control, whereas the knockdown of TRPC1 reduced the hypoxia respon se to 85 %, suggesting that TRPC6 might play a central role in mediating hypoxia response in hPASMC. However, blockade of PLC pathway caused only small inhibition of the hypoxia response. In contrast, AICAR, the agonist of AMP-activated kinase (AMPK), induced a gradual [Ca2+] i elevation, whereas compound C, an antagonist of AMPK, almost abolished the hypoxia response. Ho wever, co-immunoprecipitation revealed that AMPK α was not colocalized with TRPC6. Our data supports a role for TRPC6 in mediation of the [Ca2+] i elevation in response to hypoxia in hPASMC and suggests that this response may be linked to cellular energy status via an activation of AMPK., C. Tang, W. K To, F. Meng, Y. Wang, Y. Gu., and Obsahuje bibliografii
The aim of this contribution is to study the role of the coefficient r in the qualitative theory of the equation (r(t)Φ(y ∆))∆+p(t)Φ(y σ ) = 0, where Φ(u) = |u| α−1 sgn u with α > 1. We discuss sign and smoothness conditions posed on r, (non)availability of some transformations, and mainly we show how the behavior of r, along with the behavior of the graininess of the time scale, affect some comparison results and (non)oscillation criteria. At the same time we provide a survey of recent results acquired by sophisticated modifications of the Riccati type technique, which are supplemented by some new observations.
This paper describes the reliability analysis of a concrete containment for VVER 440 under a high internal overpressure. The probabilistic safety assessment (PSA) level 3 aims at an assessment of the probability of the concrete structure failure under the excessive overpressure. The non-linear analysis of the concrete structure was considered. The uncertainties of the loads level (long-time temperature and dead loads), the material model (concrete cracking and crushing, behavior of the reinforcement and liner), degradation effects and other influences following from the inaccuracy of the calculated model and numerical methods were taken into account in the response surface method (RSM). The results of the reliability analysis of the NPP structues are presented. and Obsahuje seznam literatury
This paper deals with a bounded control design for a class of nonlinear systems where the mathematical model may be not explicitly given. This class of uncertain nonlinear systems governed by a system of ODE with quasi-Lipschitz right-hand side and containing external perturbations as well. The Attractive Ellipsoid Method (AEM) application permits to describe the class of nonlinear feedbacks (containing a nonlinear projection operator, a linear state estimator and a feedback matrix-gain) guaranteeing a boundedness of all possible trajectories around the origin. To fulfill this property some modification of AEM are introduced: basically, some sort of sample-time corrections of the feedback parameters are required. The optimization of feedback within this class of controllers is associated with the selection of the feedback parameters which provide the trajectory converges within an ellipsoid of a "minimal size". The effectiveness of the suggested approach is illustrated by its application to a flexible arm system).
A weak form of the Henstock Lemma for the PoU-integrable functions is given. This allows to prove the existence of a scalar Volterra derivative for the PoU-integral. Also the PoU-integrable functions are characterized by means of Pettis integrability and a condition involving finite pseudopartitions.
This work provides a detailed morphological description by optic and scanning electron microscopy of the nematode Spirura dentata (Mönnig, 1938), a parasite of Herpestes ichneumon in southern and centra] Spain, together with an analyses of the morphometric variations within this population. Our findings in adult specimens include some important characters not recorded in previous descriptions, and contradict these in a few details: a) the four denticles arising from the inner wall of the oral opening are double, not simple as reported in the original description, b) four simple denticles are present, each located between the corresponding double denticles and the central protuberance of the pseudolabium, c) lateral crests are present running along the first third of the body in the adult, starting a short distance behind the deirids, and d) the pair of small submedian post-cloacal papillae mentioned in the original description has not been observed. The fourth-stage larva is described for the first time. The relative growth rate (i.e., growth rate with respect to body length) of the reproductive zone was higher in females. In adult males, the growth of the left (longer) spicule was positive, whereas that of the right (shorter) spicule was negative.