Nucleoside diphosphate kinases (NDPK) are key enzymes involved in the intracellular nucleotide maintenance in all living organisms, especially in trypanosomatids which are unable to synthesise purines de novo. Four putative NDPK isoforms were identified in the Trypanosoma cruzi Chagas, 1909 genome but only two of them were characterised so far. In this work, we studied a novel isoform from T. cruzi called TcNDPK3. This enzyme presents an atypical N-terminal extension similar to the DM10 domains. In T. cruzi, DM10 sequences targeted other NDPK isoform (TcNDPK2) to the cytoskeleton, but TcNDPK3 was localised in glycosomes despite lacking a typical peroxisomal targeting signal. In addition, TcNDPK3 was found only in the bloodstream trypomastigotes where glycolytic enzymes are very abundant. However, TcNDPK3 mRNA was also detected at lower levels in amastigotes suggesting regulation at protein and mRNA level. Finally, 33 TcNDPK3 gene orthologs were identified in the available kinetoplastid genomes. The characterisation of new glycosomal enzymes provides novel targets for drug development to use in therapies of trypanosomatid associated diseases., María de los Milagros Cámara, León Bouvier, Chantal Reigada, Fabio A. Digirolamo, Melisa Sayé, Claudio A. Pereira., and Obsahuje bibliografii
The aim of this paper was to demonstrate that it is possible to control the chaos into the Sherman system by linear feedback of own signals. After introducing of the parameter ‘α‘ in the z-equation (α → α + α1 x(t) + α2 y(t) + α3 z(t), we study how the global dynamics can be altered in a desired direction (αn are considered as free parameters). We make a detailed bifurcation investigation of the modified Sherman systems by varying the parameters αn. Finally, we calculate the maximal Lyapunov exponent, where the chaotic motion of modified Sherman system exists. and Obsahuje seznam literatury
In endoprosthesis surgery there are typically a high percentage of implant defects, these can lead to failure of the whole prosthesis. One type of total hip replacement function loss is acetabular cup loosening from the pelvic bone. This article examines manufacture perturbations as one of the possible reasons for this kind of failure. Both dimension and geometry manufacturing perturbations of ceramic head and polyethylen cup were analyzed. We find that perturbations in the variables analysed here affect considered values of contact pressure and frictional moment. Furthermore, contact pressure and frictonal moment are quantities affecting replacement success and durability. From obtained results we can recommend to fit head and cup with a clearance of between 0 mm andd 0.05 mm. We do not recommend using interference type of fit. Roundness perturbation of ceramic head should not exceed 0.025 mm. and Obsahuje seznam literatury
In current textbooks the use of Chebyshev nodes with Newton interpolation is advocated as the most efficient numerical interpolation method in terms of approximation accuracy and computational effort. However, we show numerically that the approximation quality obtained by Newton interpolation with Fast Leja (FL) points is competitive to the use of Chebyshev nodes, even for extremely high degree interpolation. This is an experimental account of the analytic result that the limit distribution of FL points and Chebyshev nodes is the same when letting the number of points go to infinity. Since the FL construction is easy to perform and allows to add interpolation nodes on the fly in contrast to the use of Chebyshev nodes, our study suggests that Newton interpolation with FL points is currently the most efficient numerical technique for polynomial interpolation. Moreover, we give numerical evidence that any reasonable function can be approximated up to machine accuracy by Newton interpolation with FL points if desired, which shows the potential of this method.
A new functional ANOVA test, with a graphical interpretation of the result, is presented. The test is an extension of the global envelope test introduced by Myllymäki et al. (2017, Global envelope tests for spatial processes, J. R. Statist. Soc. B 79, 381-404, doi: 10.1111/rssb.12172). The graphical interpretation is realized by a global envelope which is drawn jointly for all samples of functions. If a mean function computed from the empirical data is out of the given envelope, the null hypothesis is rejected with the predetermined significance level α. The advantages of the proposed one-way functional ANOVA are that it identifies the domains of the functions which are responsible for the potential rejection. We introduce two versions of this test: the first gives a graphical interpretation of the test results in the original space of the functions and the second immediately offers a post-hoc test by identifying the significant pair-wise differences between groups. The proposed tests rely on discretization of the functions, therefore the tests are also applicable in the multidimensional ANOVA problem. In the empirical part of the article, we demonstrate the use of the method by analyzing fiscal decentralization in European countries.