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27702. Trypanorhynch cestodes (Eutetrarhynchidae) from batoids along the coast of Argentina, including the description of new species in Dollfusiella Campbell et Beveridge, 1994 and Mecistobothrium Heinz et Dailey, 1974
- Creator:
- Menoret, Adriana and Ivanov, Verónica A
- Format:
- electronic, počítač, and online zdroj
- Type:
- model:article and TEXT
- Subject:
- parazitologie, parasitology, Dollfusiella acuta sp. n., Mecistobothrium oblongum sp. n., Myliobatis, Atlantoraja, Sympterygia, 2, and 59
- Language:
- English
- Description:
- a1_During a recent parasitological survey of elasmobranchs along the coast of Argentina, two new species of eutetrarhynchid cestodes of the genera Dollfusiella Campbell et Beveridge, 1994 and Mecistobothrium Heinz et Dailey, 1974 were collected from batoids. Dollfusiella acuta sp. n. was found in four arhynchobatid skates, i.e. Sympterygia acuta Garman (type host), Sympterygia bonapartii Müller et Henle, Atlantoraja castelnaui (Miranda Ribeiro) and Atlantoraja platana (Günther), and Mecistobothrium oblongum sp. n. in the eagle ray Myliobatis goodei Garman. Dollfusiella acuta sp. n. has a tentacular armature consisting of basal rows of uncinate hooks, a distinct basal swelling with uncinate, falcate and bill hooks, and a heteroacanthous metabasal armature with heteromorphous hooks (bothrial uncinate hooks and antibothrial falcate hooks), hooks 1(1') not separated, testes in two columns and an internal seminal vesicle. The tentacular armature of M. oblongum sp. n. is characterised by basal rows of uncinate hooks, a basal swelling with uncinate and falcate hooks, a typical heteroacanthous metabasal armature with heteromorphous hooks (uncinate and falcate to spiniform), and hooks 1(1') separated and of a constant size along the tentacle. It also possesses an elongate scolex, numerous testes arranged in 5-6 irregular columns, and an internal seminal vesicle. The discovery of M. oblongum in M. goodei represents the first record of species of Mecistobothrium in the southwestern Atlantic Ocean. An amended description of Dollfusiella cortezensis (Friggens et Duszynski, 2005) is also provided to clarify details of the scolex and tentacular armature. Members of Dollfusiella in the southwestern Atlantic are specific to a single host species or to a particular host family, while M. oblongum was found in a single host species., a2_Although globally some plerocerci of eutetrarhynchids have been found in teleosts, extensive examination of teleosts off the coast of Argentina suggests that the transmission pathways of these species are exclusively based on invertebrates as intermediate or paratenic hosts., Adriana Menoret, Verónica A. Ivanov., and Obsahuje bibliografii
- Rights:
- http://creativecommons.org/publicdomain/mark/1.0/ and policy:public
27703. Trypanorhynch cestodes from Indonesian coastal waters (East Indian Ocean)
- Creator:
- Palm, Harry W.
- Format:
- Type:
- model:internalpart and TEXT
- Subject:
- Trypanorhyncha, taxonomy, Dasyrhynchus, Otohothrium, Nybelinia, Sphyriocephalus, Tentacularia, ultrastructure, sensory receptor, and Indonesia
- Language:
- English
- Description:
- Results of a study on trypanorhynch ccstodes of fishes from Indonesian coastal waters are presented. A new species, Dasyrhynchus thomasi sp. n., is described, and five species are recorded which all represent new locality records: Tentacularia coryphaenae Bose, 1797; Nyhelinia africana Dollfus, 1960; Nybelinia scoliodoni (Vijayalakshmi, Vijayalakshmi et Gangadharam, 1996); Sphyriocephalus dollfusi Bussieras et Aldrin, 1968; and Otobothrium penetrans Linton, 1907, Their known ranges of distribution are extended to the East-Indian Ocean. Scanning and transmission electron microscopy was used to clarify details of the tentacular armature and surface morphology of T. coryphaenae, D. thomasi and O. penetrans. In T. coryphaenae, hook-like microtriches along the bothridial tegument are embedded in the distal cytoplasm, sometimes showing a split base. The solid tentacular hooks are embedded into a fibrillar, highly ordered tentacular wall. D. thomasi is distinguished by its characteristically shaped bothridia and a triple chainette with winged hooks on the external surface of the tentacle. Tufts of microtrichcs with ciliated sensory receptors arc regularly arranged on the bothridial surface of O. penetrans. They show similarities to sensory receptors reported from other trypanorhynch cestodes. Otobothrium pephrikos Dollfus, 1969 is considered a junior synonym for O. penetrans, and the variability of the scolex within trypanorhynch cestodes is emphasised.
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public
27704. Trypsin-like activity of membrane-bound midgut proteases from Anticarsia gemmatalis (Lepidoptera: Noctuidae)
- Creator:
- Xavier, Luciana Pereira, Oliveira, Maria Goreti Almeida, Guedes, Raul Narciso Carvalho , Santos, Agenor Valadares , and de Simones, Salvatore Giovanni
- Type:
- article, model:article, and TEXT
- Subject:
- Trypsin, proteases, velvetbean caterpillar, Lepidoptera, Noctuidae, and Anticarsia
- Language:
- English
- Description:
- Membrane-bound proteases from preparations of the midgut of 5th instar velvetbean caterpillars, Anticarsia gemmatalis (Hübner) were obtained by resuspension of the pellet obtained after 100,000 g centrifugation. As expected of trypsin-like proteases, they hydrolyzed casein and the synthetic substrates N-α-benzoyl-L-Arg-p-nitroanilidine (L-BApNA) and N-α-p-tosyl-L-Arg methyl ester (L-TAME). Higher activities were observed at 50°C, and at pH 8.5 and 8.0 for both synthetic substrates L-BApNA and L-TAME. The membrane-bound proteases were inhibited by EDTA, phenylmethan sulphonyl fluoride (PMSF), tosyl-L-lysine chloromethyl ketone (TLCK), benzamidine and aprotinin. TLCK and benzamidine were particularly active inhibitors. The KM-values obtained were 0.23 mM for L-BApNA and 92.5 µM for L-TAME. These results provide evidence for the presence of membrane-bound trypsin-like proteases in the midgut of the velvetbean caterpillar, a key soybean pest in warm climates. The interaction between A. gemmatalis digestive proteases and soybean protease inhibitors has potentially important consequences for soybean breeding programs.
- Rights:
- http://creativecommons.org/publicdomain/mark/1.0/ and policy:public
27705. TSG-6 induces apoptosis of human hypertrophic scar fibroblasts via activation of theFas/FasL signalling pathway
- Creator:
- Li, X.-Y., Li, T., Li, X.-J., Wang, J.-N., and Chen, Z.
- Format:
- bez média and svazek
- Type:
- model:article and TEXT
- Subject:
- TSG-6, hypertrophic scar fibroblast, apoptosis, and Fas/FasL
- Language:
- English
- Description:
- Tumour necrosis factor-stimulated gene 6 (TSG6) is a protective inflammatory reaction gene which is upregulated by inflammatory processes. Recent studies suggest that TSG-6 exhibits anti-scar-ring effects. However, the mechanism of TSG-6 action in the scar formation remains poorly understood. We investigated whether TSG-6 affects growth of the human hypertrophic scar fibroblasts (HSFs) via Fas/FasL signalling pathway. Cultured HSFs were transfected with a vector carrying the TSG6gene (pLVX-Puro-TSG-6) or with a vector not containing the TSG6 gene (pLVX-Puro). Untransfected HSFs served as a control group to both transfected HSFs. The expressions level of TSG-6 was up-regulated in the pLVX-Puro-TSG-6 group at the protein and mRNA level. MTT and flow cytometry were used to assess the effect of TSG-6 on the growth and apoptotic status of HSFs. Finally, qRT-PCR and western blot were used to measure the expression levels of Fas, FasL, FADD, caspase-3 and caspase-8 in each group. The apoptosis rate was significantly enhanced and the growth rate reduced in the HSFs transfected with the TSG6 gene vector. The expression levels of Fas, FasL, FADD, caspase-3 and caspase-8 were significantly raised in the TSG-6 overexpressing HSFs. It is concluded that increased expression of TSG-6 may induce apoptosis of human hypertrophic scar fibroblasts via activation of the Fas/FasL signalling pathway. and Corresponding author: Xiao-Jing Li
- Rights:
- http://creativecommons.org/publicdomain/mark/1.0/ and policy:public
27706. Tube-MPC for a class of uncertain continuous nonlinear systems with application to surge problem
- Creator:
- Ziabari, Masoud Taleb, Jahed-Motlagh, Mohammad Reza, Salahshoor, Karim, Ramezani, Amin, and Moarefianpour, Ali
- Format:
- bez média and svazek
- Type:
- model:article and TEXT
- Subject:
- robust control, adaptive control, H∞ method, tube-MPC, and surge
- Language:
- English
- Description:
- This paper presents a new robust adaptive model predictive control for a special class of continuous-time non-linear systems with uncertainty. These systems have bounded disturbances with unknown upper bound, as well as constraints on input states. An adaptive control is used in the new controller to estimate the system uncertainty. Also, to avoid the system disturbances, a H∞ method is employed to find the appropriate gain in Tube-MPC. Finally, a surge avoidance problem in centrifugal compressors is solved to show the efficiency and effectiveness of the proposed algorithm.
- Rights:
- http://creativecommons.org/publicdomain/mark/1.0/ and policy:public
27707. Tubenose goby - a discreet invader from the past goes higher
- Creator:
- Slovák Švolíková, Kristína, Števove, Barbora, Križek, Peter, Mosná, Pavlína, Fedorčák, Jakub, and Kováč, Vladimír
- Format:
- počítač and online zdroj
- Type:
- model:article and TEXT
- Subject:
- Proterorhinus semilunaris, Danube, invasion, distribution dynamics, and higher altitudes
- Language:
- English
- Description:
- The tubenose goby has been reported to be the first non-native postglacial gobiid immigrant from the Lower Danube refuges. It is thus a pioneer species that was the forerunner of the extensive invasion of Ponto-Caspian gobies that ascended the River Danube and spread across Europe a century before other goby species. It appears that recently the tubenose goby invasion has accelerated. In this paper historical data on the distribution of the tubenose goby, together with data from extensive monitoring of fish communities in Slovakia are examined to evaluate both the temporal and spatial aspects of tubenose goby distribution dynamics. Until the 1990s, the species was recorded only in the River Danube and small water bodies in the Danubian Lowland (Slovakia). Since then the tubenose goby has spread upstream into tributaries of the River Danube. It was also recorded in several streams in eastern Slovakia after 2014, and the spatial data demonstrate that the tubenose goby has been colonising new water bodies, progressing to the north and ascending rivers, reaching higher altitudes than previously reported. These findings suggest that the tubenose goby deserves attention, even after two centuries following the onset of its invasion, and its further expansion across Europe should be carefully monitored.
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public
27708. Tubifex tubifex Müll. - photosensitive organism
- Creator:
- Vytlačilová, Jitka
- Type:
- model:article and TEXT
- Language:
- English
- Rights:
- http://creativecommons.org/publicdomain/mark/1.0/ and policy:public
27709. Tumor necrosis factor α in various tissues of insulin-resistant obese koletsky rats: relations to insulin receptor characteristics
- Creator:
- Hřebíček, A., Rypka, M., Chmela, Z., Veselý, J., Magdaléna Kantorová, and Věroslav Golda
- Format:
- print, bez média, and svazek
- Type:
- article, články, model:article, and TEXT
- Subject:
- Fyziologie člověka a srovnávací fyziologie, fyziologie, physiology, tumor necrosis factor α, insulin resistance, insulin receptor protein tyrosine kinase activity, 14, and 612
- Language:
- English
- Description:
- Tumor necrosis factor a (TNFa) was found to be significantly increased in skeletal muscles and retroperitoneal fat of obese insulin-resistant Koletsky rats as compared to control Wistar rats. This increase was accompanied by a depression of insulin receptor protein tyrosine kinase (PTK) activity. Neither the insulin-binding capacity nor insulin receptor affinity were related to this TNFa increase in these tissues. In the liver, no significant changes of TNFa content and only a lowering of insulin-binding capacity were found. It is concluded that an increased TNFa content in muscles and fat (but not in the liver) contributes to insulin resistance by lowering insulin receptor protein tyrosine kinase activity, while other insulin receptor characteristics (insulin-binding capacity and affinity of insulin receptors to the hormone) do not seem to be influenced by this factor., A. Hřebíček, M. Rypka, Z. Chmela, J. Veselý, M. Kantorová, V. Golda., and Obsahuje bibliografii
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public
27710. Tuning struggle strategy in genetic algorithms for scheduling in computational grids
- Creator:
- Xhafa, Fatos, Duran , Bernat , Abraham , Ajith, and Dahal, Keshav
- Format:
- bez média and svazek
- Type:
- model:article and TEXT
- Subject:
- Genetic algorithms, scheduling, grid computing, struggle strategy, similarity measure, and tuning
- Language:
- English
- Description:
- Job Scheduling in Computational Grids is gaining importance due to the need for efficient large-scale Grid-enabled applications. Among different optimization techniques designed for the problem, Genetic Algorithm (GA) is a popular class of solution methods. As GAs are high level algorithms, specific algorithms can be designed by choosing the genetic operators as well as the evolutionary strategies such as Steady State GAs and Struggle GAs. In this paper we focus on Struggle GAs and their tuning for scheduling of independent jobs in computational grids. Our results showed that a careful hash implementation for computing the similarity of solutions was able to alleviate the computational burden of Struggle GA and perform better than standard similarity measures. This is particularly interesting for the scheduling problem in Grid systems, which due to changeability over time, has demanding time restrictions on the computation of planning the jobs to resources.
- Rights:
- http://creativecommons.org/publicdomain/mark/1.0/ and policy:public