Rubus silvae-bohemicae is described as a new regional apomictic species belonging to the subgen. Rubus sect. Rubus ser. Micantes Sudre. It is a nemophilous bramble occurring in south-western and southern Bohemia (Czech Republic) and adjacent parts of Bavaria (Germany). It differs from the somewhat similar species, R. indusiatus Focke, by having no stellate hairs on the undersides of the leaves and a lower number of shorter stalked glands and lower number of prickles on first-year stems. An illustration of the new species (including a photograph of the type specimen), a list of localities and a distribution map are presented.