Smoking during pregnancy presents health risks for both the mother and her child. In this study we followed changes in the production of steroid hormones in pregnant smokers. We focused on changes in steroidogenesis in the blood of mothers in their 37th week of pregnancy and in mixed cord blood from their newborns. The study included 88 healthy women with physiological pregnancies (17 active smokers and 71 nonsmokers). We separately analyzed hormonal changes associated with smoking according to the sex of newborns. In women with male fetuses, we found higher levels of serum cortisone, dehydroepiandrosterone (DHEA), 7α-OH-DHEA, 17-OH pregnenolone, testosterone, and androstenedione in smokers at the 37th week compared to non-smokers. In women with female fetuses, we found lower serum levels of 7β-OH-DHEA and higher androstenedione in smokers at the 37th week. We found significantly higher levels of testosterone in newborn males of smokers and higher levels of 7α-OH-DHEA in female newborns of smokers. Smoking during pregnancy induces changes in the production of steroids in both the mother and her child. These changes are different for different fetal sexes, with more pronounced changes in mothers carrying male newborns as well as in the newborn males themselves., K. Adamcová, L. Kolátorová, T. Chlupáčová, M. Šimková, H. Jandíková, A. Pařízek, L. Stárka, M. Dušková., and Obsahuje bibliografii
Acute dilation brought about by the dietary flavonoid quercetin in coronary arterioles has been described earlier, but no information is available on its chronic effects. Male Wistar rats (body weight about 190 g) were divided to two groups: the quercetin-treated group (n=22) had quercetin supplementation of approximately 30 mg/kg/day, whereas the control group (n=20) had none. After eight weeks of treatment, intramural coronary arterioles with identical passive diameters (178±14 μm and 171±9 μm) were prepared and their biomechanics and pharmacological reactivities were tested using pressure arteriography ex vivo. The spontaneous tone of quercetin-treated arteries was higher (16.5±1.9 % vs. 12.9±0.9 %), which resulted in a reduced lumen size (144±9 μm vs. 167±12 μm), thicker vascular wall (22.6±1.8 μm vs. 17.4±1.6 μm) and decreased tangential wall stress (16.8±1.1 kPa vs. 20.5±1.6 kPa) in supplemented animals (in spontaneous tone at 50 mm Hg, p<0.01 in all these comparisons). Elevated basal NO release resulted in increased endothelial dilation in quercetin-treated animals, especially at higher intraluminal pressures (10.8±2.5 % vs. 5.7±1.3 % at 70 mm Hg, p<0.01). We found remodeling of the geometry of coronary arterioles to ensure higher dilatory reserve and nitrogen monoxide production, as well as lowered elastic stress of the vessel wall., A. Monori-Kiss, F. Kiss, J. M. Restifo, E. Monos, G. L. Nadasy., and Obsahuje bibliografii
Since October 2011, the Russian GLObal NAvigation Satellite System (GLONASS) has been revitalized and is now fully operational with 24 satellites in orbit. It is critical to assess the benefits and problems of using GLONASS observations (i.e. GLONASS-only or combined Global Positioning System (GPS) and GLONASS) for precise positioning and zenith total delay (ZTD) retrieval on a global scale using precise point positioning (PPP) technique. In this contribution, extensive evaluations are conducted with Global Navigation Satellite System (GNSS) data sets collected from 251 globally distributed stations of the International GNSS Service (IGS) network in July 2016. The stations are divided into 30 groups by antenna/radome types to investigate whether there are ante nna/radome-dependent biases in position and ZTD derived from GLONASS-only PPP. The positioning results do not show obvious antenna/radome-dependent biases except the stations with JAV_RINGANT_G3T/NONE. For these stations, the averaged biases in horizontal component, especially in the north component, can achieve as high as -9.0 mm. The standard de viation (STD) and root mean square (RMS) are used as indicators of positioning repeatability and accuracy, respectively. The averaged horizontal STD and RMS of GLONASS-only PPP are comparable to GPS-only PPP, while in vertical component, those for GLONASS-only P PP are larger. Furthermore, the STD and RMS of GPS+GLONASS combined PPP solutions are the smallest in horizontal and vertical components, indicating that adding GLONASS observations can achieve better positioning performance than GPS-only PPP. With the IGS final ZTD as reference, we find that ZTD biases and accuracy of GLONASS-only are latitude - and antenna/radome-independent. The ZTD accuracy of GLONASS-only PPP is slightly worse than that of GPS-only PPP. Compared with GPS-only PPP, the ZTD accuracy is only improved by 1.3% from 7.8 to 7.7 mm by adding GLONASS observations., Feng Zhou, Shengfeng Gu, Wen Chen and Danan Dong., and Obsahuje bibliografické odkazy
This study extends our previous work by examining the effects of alpha2 -adrenoceptors under cold stimulation involving the increase of myogenic vascular oscillations as increases of very-low-frequency and low-frequency of the blood pressure variab ility. Forty-eight adult male Sprague-Dawley rats were randomly divided into four groups: vehicle; yohimbine; hexamethonium+yohimbine; guanethidine+yohimbine. Systolic blood pressure, heart rate, power spectral analysis of spontaneous blood pressure and he art rate variability and spectral coherence at very-low-frequency (0.02 to 0.2 Hz), low-frequency (0.2 to 0.6 Hz), and high-frequency (0.6 to 3.0 Hz) regions were monitored using telemetry. Key findings are as follows: 1) Cooling-induced pressor response was attenuated by yohimbine and further attenuated by hexamethonium+yohimbine and guanethidine+yohimbine, 2) Cooling-induced tachycardia response of yohimbine was attenuated by hexame - thonium+yohimbine and guanethidine+yohimbine, 3) Different patterns of p ower spectrum reaction and coherence value compared hexamethonium+yohimbine and guanethi-dine+yohimbine to yohimbine alone under cold stimulation. The results suggest that sympathetic activation of the postsynaptic alpha2-adrenoceptors causes vasoconstriction and heightening myogenic vascular oscillations, in turn, may increase blood flow to prevent tissue damage under stressful cooling challenge., Y.-H. Lin, Y.-P. Liu, Y.-C. Lin, P.-L. Lee, C.-S. Tung., and Obsahuje bibliografii
We estimated the common seasonal signal (annual oscillation) included in the Global Positioning System (GPS) vertical position time series by using Multichannel Singular Spectrum Analysis (MSSA). We employed time series from 24 International GNSS Service (IGS) stations located in Europe which contributed to the newest ITRF 2014 (International Terrestrial Reference Frame). The MSSA method has an advantage over the traditional modelling of seasonal signals by the Least-Squares Estimation (LSE) and Singular Spectrum Analysis (SSA) approaches because it can extract time-varying and common seasonal oscillations for stations located in the considered area. Having estimated the annual curve with LSE, we may make a misfit of 3 mm when a peak-to-peak variations of seasonal si gnals are to be estimated due to the time-variability of seasonal signal. A variance of data modelled as annual signal with SSA and MSSA differs of 3 % at average what proves that the MSSA-curves contain only time-varying and common seasonal signal and leave the station-specific part, local phenomena and power-law noise intact. In contrast to MSSA, these effects are modelled by SSA. The differences in spectral indices of power-law noise between MSSA and LSE esti mated with Maximum Likelihood Estimation (MLE) are closer to zero than the ones between SSA and LSE, which means that MSSA curves do not contain site-specific noise as much as the SSA curves do., Marta Gruszczynska, Anna Klos, Severine Rosat and Janusz Bogusz., and Obsahuje bibliografické odkazy
a1_With hypoxic stress, hypoxia-inducible factor-1α (HIF-1α) and vascular endothelial growth factor (VEGF) are elevated and their responses are altered in skeletal muscles of plateau animals [China Qinghai-Tibetan plateau pikas (Ochotona curzoniae )] as compared with control animals [normal lowland Sprague-Dawley (SD) rats]. The results indicate that HIF-1α and VEGF are engaged in physiological functions under hypoxic environment. The purpose of the current study was to examine the protein levels of VEGF receptor subtypes (VEGFRs: VEGFR-1, VEGFR-2 and VEGFR-3) in the end organs, namely skeletal muscle, heart and lung in response to hypoxic stress. ELISA and Western blot analysis were employed to determine HIF-1α and the protein expression of VEGFRs in control animals and plateau pikas. We further blocked HIF-1α signal to determine if HIF-1α regulates alternations in VEGFRs in those tissues. We hypothesized that responsiveness of VEGFRs in the major end organs of plateau animals is differential with insult of hypoxic stress and is modulated by low oxygen sensitive HIF-1α. Our results show that hypoxic stress induced by exposure of lower O2 for 6 h significantly increased the levels of VEGFR-2 in skeletal muscle, heart and lung and the increases were amplified in plateau pikas. Our results also demonstrate that hypoxic stress enhanced VEGFR-3 in lungs of plateau animals. Nonetheless, no significant alternations in VEGFR-1 were observed in those tissues with hypoxic stress. Moreover, we observed decreases of VEGFR-2 in skeletal muscle, heart and lung; and decreases of VEGFR-3 in lung following HIF-1α inhibition. Overall, our findings suggest that in plateau animals 1) responsiveness of VEGFRs is different under hypoxic environment; 2) amplified VEGFR-2 response appears in skeletal muscle, heart and lung, and enhanced VEGFR-3 response is mainly observed in lung; 3) HIF-1α plays a regulatory role in the levels of VEGFRs. Our results provide the underlying cellular and molecular mechanisms responsible for hypoxic environment in plateau animals, having an impact on research of physiological and ecological adaptive responses to acute or chronic hypoxic stress in humans who living at high attitude and who live at a normal sea level but suffer from hypoxic disorders., H.-C. Xie, J.-G. Li, J.-P. He., and Obsahuje bibliografii
Čeští a němečtí odborníci v oboru historie, sociální geografie, ekonomie, politologie, teologie a orientalistiky promluvili ve veřejné panelové diskusi „Uprchlíci a my: Česká očekávání a německá zkušenost“, kterou za účasti německého velvyslance Arndta Freiherra Freytaga von Loringhovena uspořádala Učená společnost ČR 19. dubna 2016 v budově Akademie věd ČR. Následná rozsáhlá debata prokázala velký zájem veřejnosti o tuto tematiku. Zveřejňujeme úvodní slovo prof. Jaroslava Pánka z Historického ústavu AV ČR v původním znění, které na diskusi muselo být vzhledem k omezenému času značně zkráceno. and Jaroslav Pánek.
Left atrial (LA) volume (LAV) is used for the selection of patients with atrial fibrillation (AF) to rhythm control strategies. Calculation of LAV from the LA diameters and areas by two-dimensional (2D) echocardiography may result in significant error. Accuracy of atrial volume assessment has never been studied in patients with long-standing persistent AF (LSPAF) and significant atrial remodeling. This study investigated correlation and agreement between 2D echocardiographic (Simpson method) and electroanatomic (CARTO, Biosense Webster) left and right atrial (RA) volumes (LAVECHO vs. LAVCARTO and RAVECHO vs. RAVCARTO) in patients undergoing catheter ablation for LSPAF. The study enrolled 173 consecutive subjects (females: 21 %, age: 59±9 years). There was only modest correlation between LAVECHO (92±31 ml) and LAVCARTO (178±37 ml) (R=0.57), and RAVECHO (71±29 ml) and RAVCARTO (173±34 ml) (R=0.42), respectively. LAVECHO and RAVECHO underestimated LAVCARTO and RAVCARTO with the absolute bias (±1.96 standard deviation) of -85 (-148; -22) ml and -102 (-169; -35) ml, respectively, and with the relative bias of -48 (-75; -21) % and -59 (-88; -30) %, respectively (all P<0.000001 for their mutual difference). Significant confounders of this difference were not identified. In patients with LSPAF, 2D echocardiography significantly underestimated both LA and RA volumes as compared with electroanatomic reference. This disagreement was independent of clinical, echocardiographic and mapping characteristics., L. Škňouřil, Š. Havránek, V. Bulková, M. Dorda, T. Paleček, J. Šimek, Z. Fingrová, A. Linhart, J. Januška, D. Wichterle, M. Fiala., and Obsahuje bibliografii