a1_Oxidized low density lipoproteins (oxLDL) formed in vivo induce a humoral immune response. Oxidative modification of LDL renders it immunogenic and a heterogeneous population of specific anti-oxLDL antibodies is produced. These antibodies could represent a biological marker of oxidative stress and serve as markers of atherosclerosis. Autoantibodies against oxLDL (oLAb) have been detected in human subjects practically of every age. oLAb also appear in the blood of pregnant women. Some studies have shown that the levels of antibodies to oxLDL were elevated in women with established preeclampsia. The present study was aimed to estimate the oLAb IgG levels in the first and second trimester of pregnancy. Furthermore, we estimated the correlation between maternal serum (MS) levels of oLAb and alpha-1-fetoprotein (MS AFP), human chorionic gonadotrophin (MS HCG) and trophoblast-specific-beta-1-glycoprotein (MS SP1), because these proteins are determined as a part of prenatal biochemical screening for fetal congenital abnormalities. Our study deals with the oLAb changes in women with pregnancy-induced hypertension. We also investigated the correlation between oLAb IgG and anticardiolipin antibodies IgG (ACA) in the serum of pregnant women. We examined 40 pregnant women attending Institute for Mother and Child Care for their antenatal care as outpatients. Routine blood samplings between the 9-13th week of pregnancy and 16-18th week of pregnancy were performed as a part of biochemical prenatal screening for fetal congenital abnormalities (Group 1). Their mean age was 27±4.1 years. Furthermore, we examined 26 women in the second or third trimester with pregnancy-induced hypertension (Group 2). Group 2 was compared with 49 pregnant women in the second or third trimester who were normotensive (Group 3)., a2_We used commercial standardized ELISA kits for determination of oLAb IgG, ACA IgG, MS AFP and MS HCG, MS SP1 was analyzed by single radial immunodiffusion. We did not find any differences in the levels of oLAb IgG in the first and second trimester in the women of Group 1. The correlation between oLAb and ACA IgG was not statistically significant (Spearman coefficient r=0.22, p=0.1). The correlation between oLAb IgG with MS AFP, MS HCG and MS SP1 was not statistically significant. Weak negative correlation for AFP and HCG was suggested both in the first and in the second trimester. The levels of oLAb IgG in the group of women with pregnancy-induced hypertension were significantly lower than in the group of normotensive women (348±388 U/ml v.s. 579±400 mU/ml, p<0.01). We can conclude that the levels of oLAb do not differ in the first and second trimester of gravidity. However, we cannot exclude the possible influence of an inverse relationship between oLAb IgG titers and the synthesis of fetoplacental antigens. This finding is important especially in the context of the results of prenatal biochemical screening. Pregnancy-induced hypertension is associated with lower levels of oLAb. Weak cross-reactivity between oLAb and anticardiolipin antibodies may exist but there is a possibility that there are two different populations of antibodies reacting with various antigens., L. Fialová, L. Mikulíková, I. Malbohan, O. Benešová, S. Štípek, T. Zima, A. Zwinger., and Obsahuje bibliografii
Studie Evy Myslivcové se zabývá korespondencí mezi hudebním skladatelem Antonínem Dvořákem a jeho blízkým přítelem Aloisem Göblem., This article introduces newly found photocopies of two letters from Antonín Dvořák to Alois Göbl with heretofore unknown and unpublished contents (newly discovered facts about Dvořák’s life and about period reception of his masterpieces – e.g. the Symphony No. 9 in E Minor “From the New World”, op. 95, and the Cello Concerto in B Minor, op. 104) and places them within the context of Dvořák’s correspondence addressed to his friend Göbl in Sychrov that has already been published in several different editions., Eva Myslivcová., Rubrika: Studie, and Německé resumé na s. 299, anglický abstrakt na s. 289.
Early consequences of lithium-pilocarpine convulsive status epilepticus (SE) were studied six days after this status had been induced in rat pups at the age of either 12 or 25 days. Studies of spontaneous EEG activity demonstrated the presence of epileptic phenomena (isolated spikes) in both hippocampus and cortex (cortical spikes were more expressed in the older group). There were no marked behavioral correlates of spikes and transition into the ictal phase was exceptional. The motor performance on a rotorod and a horizontal bar was the same in experimental and control rats of both ages. Behavior in the open field was changed in a reverse manner in the two age groups: the locomotor activity of rats with induced seizures at the age of 12 days was significantly lower than that of their control siblings, whereas animals undergoing status at the age of 25 days were hyperactive. In addition, they also exhibited increased exploratory activity (rearing) and their habituation to the open field was deranged. Nissl-stained brain sections demonstrated extensive brain damage in the older group in contrast to the negative findings in younger animals. EEG, behavioral and morphological changes induced by status epilepticus in developing rats persisted for 6 days after the status. They markedly differed according to the age of animals., L. Suchomelová, H. Kubová, R. Haugvicová, R. Druga, P. Mareš., and Obsahuje bibliografii
Insulin resistance is present in patients with Type 2 diabetes mellitus as well as in obese patients without diabetes. The aim of our study was to compare insulin action in diabetic and control persons with or without obesity and to evaluate the influence of serum cholesterol, serum triglyceride and blood pressure on metabolic variables of insulin action. We examined 42 Type 2 diabetic patients and 41 control persons with body mass index (BMI) from 21.1 to 64.5 kg.m-2, and 33 to 71 years old. The isoglycemic hyperinsulinemic clamp technique was performed at an insulin infusion rate of 1 mU.kg-1.min-1 during 120 min. We evaluated the metabolic clearance rate of glucose (MCRG, ml.kg-1.min-1) as the most important indicator of insulin action by isoglycemic clamp. The Pearson's correlation and multiple regression models were used to compare studied factors with the insulin action. We found following predictors of insulin resistance expressed in the relationship with MCRG: BMI (r = -0.68, p<0.001), plasma glucose concentration (r = -0.66, p<0.001), cholesterol (r=-0.55, p<0.001), triglycerides (r = -0.54, p<0.001) and mean blood pressure (r = -0.38, p<0.01). From the multiple regression analysis we conclude that obesity may have even greater influence on the insulin action than diabetes mellitus itself., G. Šindelka, J. Škrha, M. Prázný, T. Haas., and Obsahuje bibliografii
Studie Heleny Matějčkové se zabývá obchodními kontakty mezi hudebním skladatelem Antonínem Dvořákem a londýnským nakladatelem Augener & Co., In the course of research focusing on the history of publication of the works of A. Dvořák, printed editions have been discovered for five works that were issued in 1885 and 1886 by the London publishing house Augener & Co. The literature and sources concerning the life and work of A. Dvořák, however, make no mention of this publisher or of its editions of Dvořák’s music. The study maps the history and activity of the publishing house, devoting the greatest attention to describing the new sources and to consideration of the possible circumstances of their publication – Augener’s editions of Dvořák’s compositions appear to have been prepared on the basis of already issued printings by the Prague publisher F. A. Urbánek – and of aspects of publishing practice at the time, especially with regard to copyright issues., Helena Matějčková., Rubrika: Studie, and Anglické resumé na s. 265, anglický abstrakt 247.
The reports of analgesic effects of benzodiazepines are inconsistent. There is evidence of a hyperalgesic effect induced by activation of supraspinal GABAA receptors and an antinociceptive effect induced by activation of receptors located in the spinal cord (dorsal horns). The aim of the study was to discover whether the systemic administration of a benzodiazepine agent alprazolam increases the systemic analgesic efficacy of non-opioid analgesic ibuprofen. Experimental studies combining these agents have not yet been published. We used three experimental methods - writhing test (with acetic acid), tail-flick test and plantar test to assess analgesic action. The drugs were administered orally. Augmentation of the analgesic effect of ibuprofen by alprazolam was proved for the writhing test at a dose of 30 mg/kg of ibuprofen and alprazolam 1 mg/kg. The reaction time of the combination was significantly prolonged in comparison with ibuprofen alone. The results of the tail-flick test and plantar test were negative. The effect of ibuprofen was not enhanced by alprazolam in tests of acute thermal pain. Our results have demonstrated that the analgesic action of ibuprofen is only weakly enhanced by alprazolam., T. Doležal, M. Kršiak., and Obsahuje bibliografii
a1_Predchádzajúce výskumy naznačujú, že autenticita (pravdivosť v prežívaní a správaní) súvisí s lepším fungovaním blízkych vzťahov. Štúdia je zameraná najmä na partnerskú, resp. vzťahovú autenticitu, teda tendenciu byť autentický voči partnerovi vo vzťahu. Výskum bol zameraný na zistenie korelačných súvislostí autenticity so vzťahovou autonómiou, so sebaúctou, so spokojnosťou a mierou investícií do vzťahu, s dimenziami lásky a s mierou vnímanej prepojenosti partnerov, a to s cieľom overiť, či autenticita partnerov môže predikovať kvalitu partnerského vzťahu. V rámci partnerských dvojíc bola zisťovaná aj miera zhody medzi autenticitou žien a autenticitou mužov, a tiež to, či autenticita jedného partnera súvisí s aspektmi vzťahu sledovanými u druhého partnera (tzv. efekt partnera). Údaje boli zozbierané prostredníctvom batérie dotazníkov (Škála vzťahovej autenticity, Rolový dotazník autenticity, Dotazník autonómie v partnerskom vzťahu, Škála trojuholníku lásky, dimenzie spokojnosť a investície zo Škály investičného modelu, Rosenbergova škála sebahodnotenia a Škála zahrnutosti druhého v sebe) od 189 respondentov vo veku od 18 do 33 rokov, z toho 51 partnerských párov. Výsledky poukázali na štatisticky významné korelácie medzi partnerskou autenticitou a všetkými sledovanými aspektmi partnerského vzťahu, ako aj na nenáhodnú podobnosť partnerov v miere autenticity. Tiež sa preukázal pozitívny efekt autenticity partnera na vzťahovú autonómiu a spokojnosť druhého partnera (u oboch pohlaví). Čo sa týka dimenzií lásky, u žien bol zistený pozitívny efekt autenticity na oddanosť mužov, kým u mužov pozitívny efekt autenticity na mieru intimity žien., a2_ Určitým limitom výskumu môže byť jeho sebavýpoveďový charakter, sociálna žiaducosť sledovaných charakteristík a špecifickosť výskumnej vzorky (prevažne vysokoškolskí študenti, relatívne vysoký podiel študentov psychológie)., b1_Previous research suggests that authenticity (the truth in the experiences and behaviors) is associated with better functioning of close relationships. The study is mainly focused on the partner or relational authenticity, i.e. the tendency to be authentic towards the partner in the relationship. The research was aimed at finding out the correlations of authenticity with relational autonomy, self-esteem, satisfaction and the level of investment in the partnership, dimensions of love, and the extent of perceived connectedness between partners, to verify whether the authenticity of partners may predict the quality of partnership. Then the level of agreement between the authenticity of women and authenticity of men within the couples was assessed, as well as whether the authenticity of one partner is related to aspects of relationship of the other partner (so-called partner´s effect). Data were collected through the battery of questionnaires (Partnership authenticity scale, Role authenticity questionnaire, Autonomy in partnership questionnaire, Triangular love scale, satisfaction and investment dimensions of the Investment model scale, Rosenberg self-esteem scale, and Inclusion of other to the self scale) from 189 respondents aged 18-33 years, including 51 couples. The results showed statistically significant correlations between relational authenticity and all monitored aspects of the partnership, as well as nonrandom similarity between partners in the level of their authenticity. It was also showed the positive effect of partner’s authenticity to relational autonomy and satisfaction (in both sexes). Regarding the dimensions of love, it was found the positive effect of women’s authenticity to the men’s commitment, and positive effect of men’s authenticity to level of women’s intimacy., b2_Certain limits of the research can be its self-report character, social desirability of observed characteristics, and specificity of the research sample (mostly university students, with relatively high proportion of psychology students)., Eva Nábělková, Natália Balážová., and Obsahuje seznam literatury
We investigated the gastric response to an ulcerogenic irritant and the change in gastric functions in an experimental rat model of obstructive jaundice, with or without biliary drainage. After biliary obstruction for 14 days, rats with ligated bile duct (BDL) were randomly divided into three groups: BDL group without biliary drainage, BDL followed by choledochoduodenostomy (CD) or a choledochovesical fistula (CVF). The gastric functions were evaluated 2 weeks after the surgery. Gastric damage, induced by orogastric administration of ethanol, was evaluated 30 min later using a lesion index and microscopic scoring was then performed on fixed stomachs. Basal gastric acid secretion was measured by the pyloric ligation method.The lesion index and maximum lesion depth did not differ in the BDL and sham groups, while they were significantly reduced in the CD group. Gastric acid output and secretory volume were reduced in the BDL group compared to the sham group, while these reductions were abolished in the CD group. Afferent denervation with capsaicin further reduced the ulcer index in the later group. Our data suggest that gastric mucosal susceptibility to injury is dependent on the normal flow of bile into the duodenal lumen, which appears to be a requirement for adaptive gastric cytoprotection., A. Cingi, R. Ahiskali, B. K. Oktar, M. A. Gülpinar, C. Yegen, B.Ç. Yegen., and Obsahuje bibliografii
The two hundred and twenty-fifth anniversary of the Don Giovanni premiere, in 1787, and the one hundred and seventy-fifth anniversary of the foundation, in 1837, of Pragues Mozart Denkmal, the first ever Mozart Memorial Collection, housed from then until now in Pragues Klementinum, offer the opportunity to take a look at Mozarts standing in the early 19th century in Bohemia through the eyes of press and writing on music of the time (August Wilhelm Ambros, Wenzel Johann Tomaschek, Joseph Proksch, Ed. Murelli), as well as period arrangements of his works and the ways of their interpretation., Michaela Freemanová., Obsahuje seznam literatury, and České resumé na s. 102.